Tissue integrity and RNA quality of laser microdissected phloem of potato
| العنوان: | Tissue integrity and RNA quality of laser microdissected phloem of potato |
|---|---|
| المؤلفون: | Yueyue Yu, Coralie C. Lashbrook, David J. Hannapel |
| المصدر: | Planta. 226(3) |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Plant Stems, Lasers, fungi, food and beverages, RNA, Plant Science, Biology, Phloem, Cell morphology, Molecular biology, Cell biology, Transcriptome, Gene Expression Regulation, Plant, RNA, Plant, Genetics, RNA, Messenger, Transcription factor, Microdissection, Vascular tissue, Laser capture microdissection, Solanum tuberosum |
| الوصف: | The phloem is an important conduit for the transport of signaling molecules including RNA. Phloem sap has served as a source of RNA to profile uncontaminated phloem transcriptomes but its collection is difficult in many species. Laser capture microdissection techniques offer a valuable alternative for isolating RNA from specific vascular cells. In potato (Solanum tuberosum L.), there are seven BEL1-like transcription factors expressed throughout the plant with diverse functions. The RNA of one of these, StBEL5, moves through the phloem from the leaf to stolon tips to regulate tuber formation. In this study, the presence of several BEL RNAs and one Knotted1-like RNA was determined in phloem cells collected by laser microdissection coupled to laser pressure catapulting (LMPC). Three fixatives were compared for their effect on cell morphology and RNA quality in transverse sections of stems of potato. For optimum tissue integrity and quality of RNA from potato stem sections, the best results were achieved using ethanol acetate as the fixative. In addition, the RT-PCR results demonstrated the presence of six out of seven of the StBEL RNAs and a potato Knox RNA in phloem cells. |
| تدمد: | 0032-0935 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc14b4832d191b380a16e98a3a1af9ea https://pubmed.ncbi.nlm.nih.gov/17387510 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....dc14b4832d191b380a16e98a3a1af9ea |
| قاعدة البيانات: | OpenAIRE |
| ResultId |
1 |
|---|---|
| Header |
edsair OpenAIRE edsair.doi.dedup.....dc14b4832d191b380a16e98a3a1af9ea 764 3 unknown 764.335754394531 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsair&AN=edsair.doi.dedup.....dc14b4832d191b380a16e98a3a1af9ea&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc14b4832d191b380a16e98a3a1af9ea# [Name] => EDS - OpenAIRE [Category] => fullText [Text] => View record in OpenAIRE [MouseOverText] => View record in OpenAIRE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Tissue integrity and RNA quality of laser microdissected phloem of potato
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Yueyue+Yu%22">Yueyue Yu</searchLink><br /><searchLink fieldCode="AR" term="%22Coralie+C%2E+Lashbrook%22">Coralie C. Lashbrook</searchLink><br /><searchLink fieldCode="AR" term="%22David+J%2E+Hannapel%22">David J. Hannapel</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => <i>Planta</i>. 226(3) ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2007 ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Plant+Stems%22">Plant Stems</searchLink><br /><searchLink fieldCode="DE" term="%22Lasers%22">Lasers</searchLink><br /><searchLink fieldCode="DE" term="%22fungi%22">fungi</searchLink><br /><searchLink fieldCode="DE" term="%22food+and+beverages%22">food and beverages</searchLink><br /><searchLink fieldCode="DE" term="%22RNA%22">RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+Science%22">Plant Science</searchLink><br /><searchLink fieldCode="DE" term="%22Biology%22">Biology</searchLink><br /><searchLink fieldCode="DE" term="%22Phloem%22">Phloem</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+morphology%22">Cell morphology</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+biology%22">Molecular biology</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+biology%22">Cell biology</searchLink><br /><searchLink fieldCode="DE" term="%22Transcriptome%22">Transcriptome</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+Expression+Regulation%2C+Plant%22">Gene Expression Regulation, Plant</searchLink><br /><searchLink fieldCode="DE" term="%22RNA%2C+Plant%22">RNA, Plant</searchLink><br /><searchLink fieldCode="DE" term="%22Genetics%22">Genetics</searchLink><br /><searchLink fieldCode="DE" term="%22RNA%2C+Messenger%22">RNA, Messenger</searchLink><br /><searchLink fieldCode="DE" term="%22Transcription+factor%22">Transcription factor</searchLink><br /><searchLink fieldCode="DE" term="%22Microdissection%22">Microdissection</searchLink><br /><searchLink fieldCode="DE" term="%22Vascular+tissue%22">Vascular tissue</searchLink><br /><searchLink fieldCode="DE" term="%22Laser+capture+microdissection%22">Laser capture microdissection</searchLink><br /><searchLink fieldCode="DE" term="%22Solanum+tuberosum%22">Solanum tuberosum</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => The phloem is an important conduit for the transport of signaling molecules including RNA. Phloem sap has served as a source of RNA to profile uncontaminated phloem transcriptomes but its collection is difficult in many species. Laser capture microdissection techniques offer a valuable alternative for isolating RNA from specific vascular cells. In potato (Solanum tuberosum L.), there are seven BEL1-like transcription factors expressed throughout the plant with diverse functions. The RNA of one of these, StBEL5, moves through the phloem from the leaf to stolon tips to regulate tuber formation. In this study, the presence of several BEL RNAs and one Knotted1-like RNA was determined in phloem cells collected by laser microdissection coupled to laser pressure catapulting (LMPC). Three fixatives were compared for their effect on cell morphology and RNA quality in transverse sections of stems of potato. For optimum tissue integrity and quality of RNA from potato stem sections, the best results were achieved using ethanol acetate as the fixative. In addition, the RT-PCR results demonstrated the presence of six out of seven of the StBEL RNAs and a potato Knox RNA in phloem cells. ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 0032-0935 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc14b4832d191b380a16e98a3a1af9ea" linkWindow="_blank">https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc14b4832d191b380a16e98a3a1af9ea</link><br /><link linkTarget="URL" linkTerm="https://pubmed.ncbi.nlm.nih.gov/17387510" linkWindow="_blank">https://pubmed.ncbi.nlm.nih.gov/17387510</link> ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => CLOSED ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsair.doi.dedup.....dc14b4832d191b380a16e98a3a1af9ea ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Languages] => Array
(
[0] => Array
(
[Text] => Undetermined
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => Plant Stems
[Type] => general
)
[1] => Array
(
[SubjectFull] => Lasers
[Type] => general
)
[2] => Array
(
[SubjectFull] => fungi
[Type] => general
)
[3] => Array
(
[SubjectFull] => food and beverages
[Type] => general
)
[4] => Array
(
[SubjectFull] => RNA
[Type] => general
)
[5] => Array
(
[SubjectFull] => Plant Science
[Type] => general
)
[6] => Array
(
[SubjectFull] => Biology
[Type] => general
)
[7] => Array
(
[SubjectFull] => Phloem
[Type] => general
)
[8] => Array
(
[SubjectFull] => Cell morphology
[Type] => general
)
[9] => Array
(
[SubjectFull] => Molecular biology
[Type] => general
)
[10] => Array
(
[SubjectFull] => Cell biology
[Type] => general
)
[11] => Array
(
[SubjectFull] => Transcriptome
[Type] => general
)
[12] => Array
(
[SubjectFull] => Gene Expression Regulation, Plant
[Type] => general
)
[13] => Array
(
[SubjectFull] => RNA, Plant
[Type] => general
)
[14] => Array
(
[SubjectFull] => Genetics
[Type] => general
)
[15] => Array
(
[SubjectFull] => RNA, Messenger
[Type] => general
)
[16] => Array
(
[SubjectFull] => Transcription factor
[Type] => general
)
[17] => Array
(
[SubjectFull] => Microdissection
[Type] => general
)
[18] => Array
(
[SubjectFull] => Vascular tissue
[Type] => general
)
[19] => Array
(
[SubjectFull] => Laser capture microdissection
[Type] => general
)
[20] => Array
(
[SubjectFull] => Solanum tuberosum
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Tissue integrity and RNA quality of laser microdissected phloem of potato
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Yueyue Yu
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Coralie C. Lashbrook
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => David J. Hannapel
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 12
[M] => 01
[Type] => published
[Y] => 2007
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 00320935
)
[1] => Array
(
[Type] => issn-locals
[Value] => edsair
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 226
)
[1] => Array
(
[Type] => issue
[Value] => 3
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Planta
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |