Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows
| العنوان: | Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows |
|---|---|
| المؤلفون: | João Henrique Moreira Viana, Adriana Cortez, André Penido Oliveira, Paula Maria Pires do Nascimento, Rômulo Cerqueira Leite, Marcos Bryan Heinemann |
| المصدر: | Pesquisa Agropecuária Brasileira, Vol 51, Iss 5, Pp 676-679 (2016) Pesquisa Agropecuária Brasileira, Volume: 51, Issue: 5, Pages: 676-679, Published: MAY 2016 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Pesquisa Agropecuária Brasileira v.51 n.5 2016 Pesquisa Agropecuária Brasileira Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| بيانات النشر: | FapUNIFESP (SciELO), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Agriculture (General), TRANSMISSÃO DE DOENÇAS ANIMAIS, fluido folicular, Bos indicus, Biology, Virus, S1-972, 03 medical and health sciences, DNA viral, 0302 clinical medicine, Infectious bovine rhinotracheitis virus, transmission risk, Serum neutralization test, Dna viral, vírus da rinotraqueíte infecciosa bovina, 030219 obstetrics & reproductive medicine, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Infectious bovine rhinotracheitis, 040201 dairy & animal science, Follicular fluid, Virology, follicular fluid, viral DNA, Bovine herpesvirus, Animal Science and Zoology, Agronomy and Crop Science, infectious bovine rhinotracheitis virus, risco de transmissão |
| الوصف: | The objective of this work was to investigate the presence of bovine herpesvirus type 1 (BoHV-1) in follicular fluid and in cumulus-oocyte complexes (COC) recovered from naturally infected cows but with no clinical signs of the disease. Cows that were seropositive (n=38) or seronegative (n=8, control) to infectious bovine rhinotracheitis were selected after a serum neutralization test in microplates. The presence of the virus was investigated by PCR in COC and in follicular fluid. Viral DNA was not found in any of the samples. The obtained results suggest that serologically positive cows with no clinical signs of the disease offer negligible risk of transmitting BoHV-1 by COC or follicular fluid. Resumo: O objetivo deste trabalho foi investigar a presença do herpesvírus bovino tipo 1 (BoHV-1) no fluido folicular e em complexos cumulus-oócito (COC) coletados de vacas naturalmente infectadas mas sem sinais clínicos da doença. Vacas soropositivas (n=38) ou soronegativas (n=8, controle) para o vírus da rinotraqueíte infecciosa bovina foram selecionadas por prova de soroneutralização em microplacas. A presença do vírus foi investigada por PCR em COC e fluido folicular. O DNA viral não foi encontrado em qualquer das amostras. Os resultados obtidos são indicativos de que vacas soropositivas, mas sem sinais clínicos da doença, oferecem risco negligível de transmissão do BoHV-1 por COC ou fluido folicular. |
| وصف الملف: | text/html |
| تدمد: | 0100-204X |
| DOI: | 10.1590/s0100-204x2016000500031 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7cd67da79d81bfe9bf6ee6d61288de8 https://doi.org/10.1590/s0100-204x2016000500031 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d7cd67da79d81bfe9bf6ee6d61288de8 |
| قاعدة البيانات: | OpenAIRE |
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Cows that were seropositive (n=38) or seronegative (n=8, control) to infectious bovine rhinotracheitis were selected after a serum neutralization test in microplates. The presence of the virus was investigated by PCR in COC and in follicular fluid. Viral DNA was not found in any of the samples. The obtained results suggest that serologically positive cows with no clinical signs of the disease offer negligible risk of transmitting BoHV-1 by COC or follicular fluid. Resumo: O objetivo deste trabalho foi investigar a presença do herpesvírus bovino tipo 1 (BoHV-1) no fluido folicular e em complexos cumulus-oócito (COC) coletados de vacas naturalmente infectadas mas sem sinais clínicos da doença. Vacas soropositivas (n=38) ou soronegativas (n=8, controle) para o vírus da rinotraqueíte infecciosa bovina foram selecionadas por prova de soroneutralização em microplacas. A presença do vírus foi investigada por PCR em COC e fluido folicular. 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