Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus
| العنوان: | Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus |
|---|---|
| المؤلفون: | Xin Y. Yuan, Maya Andonova, Frank Plummer, Anton Andonov, Jody D. Berry, Darrell Johnstone, Michael J. Gubbins, Mike Drebot |
| المصدر: | Molecular Immunology |
| بيانات النشر: | Elsevier BV, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | CoV, coronavirus, VH, variable region heavy chain, Immunogenetics, medicine.disease_cause, Neutralization, F.C.A., Freund’s complete adjuvant, Mice, Antibody Specificity, Murine, Neutralizing, Peptide sequence, D, diversity, Phylogenetic analysis, biology, Antibodies, Monoclonal, Severe acute respiratory syndrome-related coronavirus, Antibody, Immunoglobulin Heavy Chains, Monoclonal antibody, medicine.drug_class, Viral protein, Molecular Sequence Data, Immunology, I.P., intraperitoneal, F.I.A., Freund’s incomplete adjuvant, Immunoglobulin light chain, Article, Evolution, Molecular, FR, framework, PBS, phosphate buffered saline, VL, variable region light chain, Antigen, Neutralization Tests, CDR, complementarity determining region, medicine, Animals, SARS, severe acute respiratory syndrome, Amino Acid Sequence, J, joining, Molecular Biology, Hybridomas, fungi, Ig, immunoglobulin, Virology, SARS-coronavirus, S.C., subcutaneous, biology.protein, Immunoglobulin Light Chains |
| الوصف: | The availability of monoclonal antibodies (mAbs) specific for the SARS-coronavirus (SARS-CoV) is important for the development of both diagnostic tools and treatment of infection. A molecular characterization of nine monoclonal antibodies raised in immune mice, using highly purified, inactivated SARS-CoV as the inoculating antigen, is presented in this report. These antibodies are specific for numerous viral protein targets, and six of them are able to effectively neutralize SARS-CoV in vitro, including one with a neutralizing titre of 0.075 nM. A phylogenetic analysis of the heavy and light chain sequences reveals that the mAbs share considerable homology. The majority of the heavy chains belong to a single Ig germline V-gene family, while considerably more sequence variation is evident in the light chain sequences. These analyses demonstrate that neutralization ability can be correlated with specific murine V(H)-gene alleles. For instance, one evident trend is high sequence conservation in the V(H) chains of the neutralizing mAbs, particularly in CDR-1 and CDR-2. The results suggest that optimization of murine mAbs for neutralization of SARS-CoV infection will likely be possible, and will aid in the development of diagnostic tools and passive treatments for SARS-CoV infection. |
| تدمد: | 0161-5890 |
| DOI: | 10.1016/j.molimm.2004.06.032 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d648f3a3bf6520108e7870e5d27a631d https://doi.org/10.1016/j.molimm.2004.06.032 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d648f3a3bf6520108e7870e5d27a631d |
| قاعدة البيانات: | OpenAIRE |
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