Effects of PTHrP on chondrocytes of sika deer antler
| العنوان: | Effects of PTHrP on chondrocytes of sika deer antler |
|---|---|
| المؤلفون: | Xue-Chao Tian, Dang-Dang Li, Shou-Tang Wang, Qu-Yuan Wang, Zhan-Peng Yue, Cui-Cui Duan, Bin Guo |
| المصدر: | Cell and Tissue Research. 354:451-460 |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | musculoskeletal diseases, medicine.medical_specialty, animal structures, Histology, Cellular differentiation, Type II collagen, Antlers, Core Binding Factor Alpha 1 Subunit, Biology, Chondrocyte, Pathology and Forensic Medicine, Chondrocytes, Cyclin D1, Internal medicine, medicine, Animals, Perichondrium, RNA, Messenger, Cells, Cultured, Cell Proliferation, Receptor, Parathyroid Hormone, Type 1, Deer, Cartilage, Parathyroid Hormone-Related Protein, Cell Differentiation, Cell Biology, musculoskeletal system, Antler, Cell biology, RUNX2, Endocrinology, medicine.anatomical_structure, Gene Expression Regulation, Proto-Oncogene Proteins c-bcl-2, hormones, hormone substitutes, and hormone antagonists |
| الوصف: | Parathyroid-hormone-related peptide (PTHrP) is an important regulator of chondrocyte differentiation in growth plates but little is known about its role in deer antler cartilage. The aim of the present study was to use the deer antler as a model to determine the possible role of PTHrP in regulating chondrocyte differentiation of deer antler. PTHrP and its receptor PTH1R mRNA were highly expressed in the perichondrium and cartilage of sika deer antler, as shown by in situ hybridization. Chondrocytes of deer antler were identified by toluidine blue staining of glycosaminoglycan and immunocytochemical staining of type II collagen (Col II). Treatment with PTHrP (1-34) reduced the expression of prehypertrophic chondrocyte marker Col IX and hypertrophic chondrocyte marker Col X. In order to confirm the mechanism of action of PTHrP, we initially examined the expression of cyclin D1, Bcl-2 and runt-related transcription factor 2 (Runx2) in sika deer antler by in situ hybridization and found that cyclin D1, Runx2 and Bcl-2 mRNA were also expressed in antler chondrocytes. Exogenous PTHrP induced the expression of cyclin D1 and Bcl-2 mRNA by various signalling pathways, whereas it inhibited Runx2 expression through PKA, p38MAPK, MEK and PI3K signalling pathways. Thus, PTHrP might promote the proliferation of antler chondrocytes and prevent their differentiation; it might furthermore influence the growth and development of sika deer antler. |
| تدمد: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-013-1670-2 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce81c5078803f4c1ab97d9e67db235c1 https://doi.org/10.1007/s00441-013-1670-2 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....ce81c5078803f4c1ab97d9e67db235c1 |
| قاعدة البيانات: | OpenAIRE |
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