Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase
| العنوان: | Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase |
|---|---|
| المؤلفون: | Karen Dalle, Bruno Coutard, Barbara Selisko, Silvia Spinelli, Christian Cambillau, M P Egloff, Arnaud Gruez, Sacha Grisel, Johan Neyts, Valérie Campanacci, Ilham Jabafi, Bruno Canard, Armando M. De Palma |
| بيانات النشر: | International Union of Crystallography, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Myocarditis, viruses, Biophysics, Picornaviridae, RNA-dependent RNA polymerase, Biology, Coxsackievirus, medicine.disease_cause, Biochemistry, Sudden death, Virus, Microbiology, chemistry.chemical_compound, Structural Biology, RNA polymerase, Genetics, medicine, Poliovirus, virus diseases, Gene Expression Regulation, Bacterial, biochemical phenomena, metabolism, and nutrition, Condensed Matter Physics, biology.organism_classification, medicine.disease, RNA-Dependent RNA Polymerase, Virology, Enterovirus B, Human, chemistry, Crystallization Communications, Crystallization |
| الوصف: | The Picornaviridae virus family contains a large number of human pathogens such as poliovirus, hepatitis A virus and rhinoviruses. Amongst the viruses belonging to the genus Enterovirus, several serotypes of coxsackievirus coexist for which neither vaccine nor therapy is available. Coxsackievirus B3 is involved in the development of acute myocarditis and dilated cardiomyopathy and is thought to be an important cause of sudden death in young adults. Here, the first crystal of a coxsackievirus RNA-dependent RNA polymerase is reported. Standard crystallization methods yielded crystals that were poorly suited to X-ray diffraction studies, with one axis being completely disordered. Crystallization was improved by testing crystallization solutions from commercial screens as additives. This approach yielded crystals that diffracted to 2.1 A resolution and that were suitable for structure determination. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc8d1081f7a382841dceb83b6030f747 https://europepmc.org/articles/PMC2335076/ |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bc8d1081f7a382841dceb83b6030f747 |
| قاعدة البيانات: | OpenAIRE |
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