Ceramide induces a multicomponent intracellular calcium increase triggering the acrosome secretion in human sperm
| العنوان: | Ceramide induces a multicomponent intracellular calcium increase triggering the acrosome secretion in human sperm |
|---|---|
| المؤلفون: | Silvia A. Belmonte, Gerardo A. De Blas, Cintia Celina Vaquer, Martin Alejandro Pavarotti, Laila Suhaiman |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, Ceramide, Cytoplasm, Vesicle-Associated Membrane Protein 2, chemistry.chemical_element, Calcium, Ceramides, CALCIUM, Exocytosis, purl.org/becyt/ford/1 [https], 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, EXOCYTOSIS, Humans, Calcium Signaling, purl.org/becyt/ford/1.6 [https], Molecular Biology, Ceramide synthase, Calcium signaling, Protein Tyrosine Phosphatase, Non-Receptor Type 1, 030219 obstetrics & reproductive medicine, CERAMIDE, Ryanodine receptor, Chemistry, Calcium channel, Acrosome Reaction, Secretory Vesicles, Ryanodine Receptor Calcium Release Channel, Cell Biology, Lipid signaling, Spermatozoa, Cell biology, 030104 developmental biology, LIPID SIGNALING, ACROSOME REACTION, Fertilization, Calcium Channels, Acrosome, SPERM |
| الوصف: | Exocytosis of spermatozoon's secretory vesicle, named acrosome reaction (AR), is a regulated event that plays a central role in fertilization. It is coupled to a complex calcium signaling. Ceramide is a multitasking lipid involved in exocytosis. Nevertheless, its effect on secretion is controversial and the underlying cellular and molecular mechanisms remain unknown. Human spermatozoa are useful to dissect the role of ceramide in secretion given that the gamete is not capable to undergo any trafficking mechanisms other than exocytosis. We report for the first time, the presence of sphingolipid metabolism enzymes such as neutral-sphingomyelinase and ceramide synthase in sperm. Ceramidases are also present and active. Both the addition of cell-permeable ceramide and the rise of the endogenous one, increase intracellular calcium acting as potent inducers of exocytosis. Ceramide triggers AR in capacitated spermatozoa and enhances the gamete response to progesterone. The lipid induces physiological ultrastructural changes in the acrosome and triggers an exocytosis-signaling cascade involving protein tyrosine phosphatase 1B and VAMP2. Real-time imaging showed an increment of calcium in the cytosol upon ceramide treatment either in the absence or in the presence of extracellular calcium. Pharmacological experiments demonstrate that at early stages the process involves ryanodine receptors, CatSper (calcium channel of sperm), and store-operated calcium channels. We set out the signaling sequence of events that connect ceramide to internal calcium mobilization and external calcium signals during secretion. These results allow the coordination of lipids and proteins in a pathway that accomplishes secretion. Our findings contribute to the understanding of ceramide's role in regulated exocytosis and fertilization. Fil: Vaquer, Cintia Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Suhaiman, Laila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentina Fil: Pavarotti, Martin Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: de Blas, Gerardo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Belmonte, Silvia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina |
| وصف الملف: | application/pdf |
| تدمد: | 1879-2596 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b97698291bd924f4cc6149f086a2f156 https://pubmed.ncbi.nlm.nih.gov/32194132 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b97698291bd924f4cc6149f086a2f156 |
| قاعدة البيانات: | OpenAIRE |
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