Use of Allogeneic Hypoxic Mesenchymal Stem Cells For Treating Disc Degeneration in Rabbits
| العنوان: | Use of Allogeneic Hypoxic Mesenchymal Stem Cells For Treating Disc Degeneration in Rabbits |
|---|---|
| المؤلفون: | Shih-Chieh Hung, Ming Chau Chang, Jung Pan Wang, En Rung Chiang, Hsiao Li Ma, Tain Hsiung Chen, Chien Lin Liu |
| المصدر: | Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 37(6) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Bone Morphogenetic Protein 7, 0206 medical engineering, 02 engineering and technology, Intervertebral Disc Degeneration, Mesenchymal Stem Cell Transplantation, Extracellular matrix, 03 medical and health sciences, 0302 clinical medicine, In vivo, medicine, Animals, Transplantation, Homologous, Orthopedics and Sports Medicine, Collagen Type II, 030203 arthritis & rheumatology, business.industry, Mesenchymal stem cell, Hypoxia (medical), Endoglin, Chondrogenesis, 020601 biomedical engineering, Immunohistochemistry, Cell Hypoxia, Bone morphogenetic protein 7, medicine.anatomical_structure, Bone marrow, Rabbits, medicine.symptom, business, Collagen Type X |
| الوصف: | Intervertebral discs (IVDs) are important biomechanical components of the spine. Once degenerated, mesenchymal stem cell (MSC)-based therapies may aid in the repair of these discs. Although hypoxic preconditioning enhances the chondrogenic potential of MSCs, it is unknown whether bone marrow MSCs expanded under hypoxic conditions (1% O2 , here referred to as hypoxic MSCs) are better than bone marrow MSCs expanded under normoxic conditions (air, here referred to as normoxic MSCs) with regards to disc regeneration capacity. The purpose of this study was to compare the therapeutic effects of hypoxic and normoxic MSCs in a rabbit needle puncture degenerated disc model after intra-disc injection. Six weeks after needle puncture, MSCs were injected into the IVD. A vehicle-treated group and an un-punctured sham-control group were included as controls. The tissues were analyzed by histological and immunohistochemical methods 6 and 12 weeks post-injection. At 6 and 12 weeks, less disc space narrowing was evident in the hypoxic MSC-treated group compared to the normoxic MSC-treated group. Significantly better histological scores were observed in the hypoxic MSC group. Discs treated with hypoxic MSCs also demonstrated significantly better extracellular matrix deposition in type II and XI collagen. Increased CD105 and BMP-7 expression were also observed upon injection of hypoxic MSCs. In conclusion, hypoxic MSC injection was more effective than normoxic MSC injection for reducing IVD degeneration progression in vivo. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1440-1450, 2019. |
| تدمد: | 1554-527X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab2aadc9edfa5c0d64c562e4399867ae https://pubmed.ncbi.nlm.nih.gov/31062869 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ab2aadc9edfa5c0d64c562e4399867ae |
| قاعدة البيانات: | OpenAIRE |
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