Proteome-Wide Zika Virus CD4 T Cell Epitope and HLA Restriction Determination
| العنوان: | Proteome-Wide Zika Virus CD4 T Cell Epitope and HLA Restriction Determination |
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| المؤلفون: | Alessandro Sette, Kerry J. Laing, Christopher L. McClurkan, Michael Gale, Elise Snoey, Rosemary J. Boyton, Lichun Dong, Daniel M. Altmann, Justin A Roby, Cecilia S. Lindestam Arlehamn, Victoria L. Campbell, LeAnn Nguyen, David M. Koelle, Phillip J. Norris, Michael P. Busch, Mars Stone |
| المساهمون: | Medical Research Council (MRC), Medical Research Council, National Institutes of Health, Innovate UK |
| المصدر: | Immunohorizons ImmunoHorizons, vol 4, iss 8 |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, CD4-Positive T-Lymphocytes, Male, Proteome, T cell, Immunology, Epitopes, T-Lymphocyte, Context (language use), Cross Reactions, Epitope, Virus, Article, Zika virus, Vaccine Related, Epitopes, Young Adult, Clinical Research, Biodefense, Chlorocebus aethiops, medicine, Immunology and Allergy, 2.1 Biological and endogenous factors, Animals, Humans, Aetiology, Pathogen, Vero Cells, Aged, biology, Zika Virus Infection, Prevention, General Medicine, Zika Virus, Middle Aged, biology.organism_classification, Virology, Vector-Borne Diseases, medicine.anatomical_structure, Infectious Diseases, Emerging Infectious Diseases, Good Health and Well Being, T-Lymphocyte, Polyclonal antibodies, biology.protein, Immunization, Female, Infection, Biotechnology |
| الوصف: | Zika virus (ZIKV) is a mosquito-borne pathogen that caused an epidemic in 2015–2016. ZIKV-specific T cell responses are functional in animal infection models, and helper CD4 T cells promote avid Abs in the vaccine context. The small volumes of blood available from field research limit the determination of T cell epitopes for complex microbes such as ZIKV. The goal of this project was efficient determination of human ZIKV CD4 T cell epitopes at the whole proteome scale, including validation of reactivity to whole pathogen, using small blood samples from convalescent time points when T cell response magnitude may have waned. Polyclonal enrichment of candidate ZIKV-specific CD4 T cells used cell-associated virus, documenting that T cells in downstream peptide analyses also recognize whole virus after Ag processing. Sequential query of bulk ZIKV-reactive CD4 T cells with pooled/single ZIKV peptides and molecularly defined APC allowed precision epitope and HLA restriction assignments across the ZIKV proteome and enabled discovery of numerous novel ZIKV CD4 T cell epitopes. The research workflow is useful for the study of emerging infectious diseases with a very limited human blood sample availability. |
| وصف الملف: | application/pdf |
| تدمد: | 2573-7732 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f3bff020405b9a60c5b313a268f9723 https://pubmed.ncbi.nlm.nih.gov/32753403 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9f3bff020405b9a60c5b313a268f9723 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25737732 |
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