SAT-297 Pituitary Hormonal Levels and Gonadal Histology in the Pubertal Period of the Ames Mice
| العنوان: | SAT-297 Pituitary Hormonal Levels and Gonadal Histology in the Pubertal Period of the Ames Mice |
|---|---|
| المؤلفون: | Luciani R. Carvalho, Juliana M Silva, Berenice B. Mendonca, Bruna A Viscardi |
| المصدر: | Journal of the Endocrine Society |
| بيانات النشر: | The Endocrine Society, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | endocrine system, Neuroendocrinology and Pituitary, Gonadal histology, business.industry, Endocrinology, Diabetes and Metabolism, Period (gene), Physiology, Medicine, Hypothalamic-Pituitary Development and Function, business, AcademicSubjects/MED00250, Hormone |
| الوصف: | Introduction: The pituitary gland controls several mechanisms as metabolism, growth, and reproduction, in response to hypothalamic stimuli. The adequate temporal/ spatial expression of transcription factors is mandatory for a normal pituitary development. PROP1 transcription factor is widely known as a key pituitary regulator. The Ames mice is a model of congenital hypopituitarism due to a pathogenic variant in the Prop1 gene, leading to growth retardation, infertility, and hypothyroidism. Aim: To characterize the peripheral levels of pituitary hormones and correlate with gonadal histology during the pubertal period of the Ames mice. Methods: Weight and naso-anal length were measured. Peripheral blood samples were collected from 5 wild type (WT) and 5 Prop1 mutants (Mut) animals with 30 (P30), 40, (P40), and 60 (P60) days after birth. Pituitary hormone levels were measured using the kit Milliplex Map® - Mouse Pituitary Bead Panel (Merck Millipore, Massachusetts, USA). Ovaries and testis from 3 WT and 3 Mut animals from each sex were collected and fixed in 4% paraformaldehyde and embedded in paraffin. Gonadal sections of 3 μm were obtained and the slices were stained with hematoxylin and eosin. Follicles were counted and classified according to the size and cellular composition as small follicle (17μm to 28μm), developing follicle (100μm), and antral follicle (>550μm). Testis were classified using the Johnsen score, ranging from 1 to 10 according to cellular composition and spermatogenesis state. Results: All mutant mice presented decreased weight and naso-anal length at the three analyzed periods. At P30, the female mutants presented GH, LH, and TSH levels similar to wild type and decreased FSH and PRL levels, as well as the males that only differed from GH reduced levels. At P40, females and males presented GH, LH, FSH, and TSH levels similar to wild type with reduced PRL levels. At P60, it was observed decreased GH, FSH, and PRL levels for both sexes and TSH levels with no difference from WT, LH secretion was decreased in mutant females and normal in males compared to WT. There was no significant difference in follicular number and classification between mutants and wild type in all analyzed periods, despite the observation of ovarian hypoplasia in the mutants. Johnsen score was in the average of 7.5 in mutant males and 8.8 in WT, with no significant difference between periods. Conclusion: Despite the absence of PROP1 and the hypopituitary phenotype, mutant females and males were able to secrete some pituitary hormones, mainly during puberty, a pituitary high demand period. Although the ovaries and testis from mutant mice are hypoplastic, cellular morphology and classification are similar to WT. |
| تدمد: | 2472-1972 |
| DOI: | 10.1210/jendso/bvaa046.1570 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90d273adb13e2fa12a49f6ed7db74a65 https://doi.org/10.1210/jendso/bvaa046.1570 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....90d273adb13e2fa12a49f6ed7db74a65 |
| قاعدة البيانات: | OpenAIRE |
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The adequate temporal/ spatial expression of transcription factors is mandatory for a normal pituitary development. PROP1 transcription factor is widely known as a key pituitary regulator. The Ames mice is a model of congenital hypopituitarism due to a pathogenic variant in the Prop1 gene, leading to growth retardation, infertility, and hypothyroidism. Aim: To characterize the peripheral levels of pituitary hormones and correlate with gonadal histology during the pubertal period of the Ames mice. Methods: Weight and naso-anal length were measured. Peripheral blood samples were collected from 5 wild type (WT) and 5 Prop1 mutants (Mut) animals with 30 (P30), 40, (P40), and 60 (P60) days after birth. Pituitary hormone levels were measured using the kit Milliplex Map® - Mouse Pituitary Bead Panel (Merck Millipore, Massachusetts, USA). Ovaries and testis from 3 WT and 3 Mut animals from each sex were collected and fixed in 4% paraformaldehyde and embedded in paraffin. Gonadal sections of 3 μm were obtained and the slices were stained with hematoxylin and eosin. Follicles were counted and classified according to the size and cellular composition as small follicle (17μm to 28μm), developing follicle (100μm), and antral follicle (>550μm). Testis were classified using the Johnsen score, ranging from 1 to 10 according to cellular composition and spermatogenesis state. Results: All mutant mice presented decreased weight and naso-anal length at the three analyzed periods. At P30, the female mutants presented GH, LH, and TSH levels similar to wild type and decreased FSH and PRL levels, as well as the males that only differed from GH reduced levels. At P40, females and males presented GH, LH, FSH, and TSH levels similar to wild type with reduced PRL levels. At P60, it was observed decreased GH, FSH, and PRL levels for both sexes and TSH levels with no difference from WT, LH secretion was decreased in mutant females and normal in males compared to WT. There was no significant difference in follicular number and classification between mutants and wild type in all analyzed periods, despite the observation of ovarian hypoplasia in the mutants. Johnsen score was in the average of 7.5 in mutant males and 8.8 in WT, with no significant difference between periods. Conclusion: Despite the absence of PROP1 and the hypopituitary phenotype, mutant females and males were able to secrete some pituitary hormones, mainly during puberty, a pituitary high demand period. 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