Polymorphism of rplF and tufB genes: expanding the MLST scheme for 'flavescence dorée' phytoplasma typing in Croatia
| العنوان: | Polymorphism of rplF and tufB genes: expanding the MLST scheme for 'flavescence dorée' phytoplasma typing in Croatia |
|---|---|
| المؤلفون: | Jelena Plavec, Ivan Knezovic, Martina Šeruga Musić, Xavier Foissac |
| المساهمون: | Department of Biology, Faculty of Science, University of Isfahan, Croatian Agency for Agriculture and Food, Partenaires INRAE, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 |
| المصدر: | Phytopathogenic Mollicutes 4. meeting of the International Phytoplasmologist Working Group 4. meeting of the International Phytoplasmologist Working Group, Sep 2019, Valencia, Spain. ⟨10.5958/2249-4677.2019.00033.1⟩ |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, Microbiology (medical), Genetics, phylogenetic analysis, grapevine yellows, rplF, Flavescence doree phytoplasma, Cell Biology, grapevine yellows, FD phytoplasma, MLST, rplF, tufB, phylogenetic analysis, Biology, 010603 evolutionary biology, 01 natural sciences, tufB, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, 010602 entomology, Infectious Diseases, FD phytoplasma, Multilocus sequence typing, Parasitology, Typing, Gene, Ecology, Evolution, Behavior and Systematics, ComputingMilieux_MISCELLANEOUS, MLST |
| الوصف: | In Croatia, major agents associated with grapevine yellows diseases (GY) are the “bois noir” (BN ; ‘Candidatus Phytoplasma solani’) and the “flavescence dorée” (FD) phytoplasmas, with the latter emerging and spreading extensively in the last decade. The aim of this study was to perform genotyping of selected FD phytoplasma strains by analysis of the housekeeping genes rplF and tufB in order to expand the currently used multilocus sequence typing (MLST) scheme. The analysis included samples of grapevine, wild plants from the vicinity of vineyards and insect-vectors from continental Croatia and Istria county. Fragments of rplF and tufB genes were amplified by PCR and sequenced. The alignment and phylogenetic analysis revealed the presence of 5 and 6 genotypes, respectively. The gene rplF showed to be more conserved than the tufB gene, but both were proved as good candidates molecular markers in FD phytoplasma genotyping due to their variability. Implementation of data on rplF and tufB genotypes in the currently used MLST scheme for FD phytoplasma differentiation would give new possibilities for tracking and tracing this quarantine pathogen. |
| اللغة: | English |
| DOI: | 10.5958/2249-4677.2019.00033.1⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e7d36a027ccfceb9682225266c957e9 https://www.bib.irb.hr/1026590 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8e7d36a027ccfceb9682225266c957e9 |
| قاعدة البيانات: | OpenAIRE |
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