Impact of whole-body versus nose-only inhalation exposure systems on systemic, respiratory, and cardiovascular endpoints in a 2-month cigarette smoke exposure study in the ApoE
| العنوان: | Impact of whole-body versus nose-only inhalation exposure systems on systemic, respiratory, and cardiovascular endpoints in a 2-month cigarette smoke exposure study in the ApoE |
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| المؤلفون: | Walter K. Schlage, Sin Kei Wong, Justyna Szostak, Francesco Lucci, Ee Tsin Wong, Patrick Vanscheeuwijck, Yang Xiang, Julia Hoeng, Ulrike Kogel, Manuel C. Peitsch, Wei Teck Tan, Nikolai V. Ivanov, Patrice Leroy, Arkadiusz K. Kuczaj, Bjoern Titz, Emmanuel Guedj, Tiffany Low |
| المصدر: | Journal of Applied Toxicology |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Apolipoprotein E, Lung Diseases, Male, Apolipoprotein B, Physiology, Inflammation, 010501 environmental sciences, Toxicology, 01 natural sciences, Cigarette Smoking, Nicotine, 03 medical and health sciences, Mice, Smoke, medicine, Animals, Sidestream smoke, Respiratory system, Research Articles, 030304 developmental biology, 0105 earth and related environmental sciences, nose‐only inhalation, 0303 health sciences, Inhalation Exposure, Lung, biology, business.industry, cigarette smoke, Respiratory disease, whole‐body inhalation, medicine.disease, Absorption, Physiological, Disease Models, Animal, medicine.anatomical_structure, Apolipoproteins, Cardiovascular Diseases, biology.protein, respiratory tract effects, medicine.symptom, business, cardiovascular effects, medicine.drug, Research Article |
| الوصف: | Cigarette smoking is one major modifiable risk factor in the development and progression of chronic obstructive pulmonary disease and cardiovascular disease. To characterize and compare cigarette smoke (CS)‐induced disease endpoints after exposure in either whole‐body (WB) or nose‐only (NO) exposure systems, we exposed apolipoprotein E‐deficient mice to filtered air (Sham) or to the same total particulate matter (TPM) concentration of mainstream smoke from 3R4F reference cigarettes in NO or WB exposure chambers (EC) for 2 months. At matching TPM concentrations, we observed similar concentrations of carbon monoxide, acetaldehyde, and acrolein, but higher concentrations of nicotine and formaldehyde in NOEC than in WBEC. In both exposure systems, CS exposure led to the expected adaptive changes in nasal epithelia, altered lung function, lung inflammation, and pronounced changes in the nasal epithelial transcriptome and lung proteome. Exposure in the NOEC caused generally more severe histopathological changes in the nasal epithelia and a higher stress response as indicated by body weight decrease and lower blood lymphocyte counts compared with WB exposed mice. Erythropoiesis, and increases in total plasma triglyceride levels and atherosclerotic plaque area were observed only in CS‐exposed mice in the WBEC group but not in the NOEC group. Although the composition of CS in the breathing zone is not completely comparable in the two exposure systems, the CS‐induced respiratory disease endpoints were largely confirmed in both systems, with a higher magnitude of severity after NO exposure. CS‐accelerated atherosclerosis and other pro‐atherosclerotic factors were only significant in WBEC. To characterize and compare cigarette smoke‐induced disease endpoints after exposure in either whole‐body (WB) or nose‐only (NO) exposure chambers (EC), we exposed apolipoprotein E‐deficient mice to filtered air or mainstream smoke from 3R4F reference cigarettes (same target total particulate matter concentration) in NOEC or WBEC for 2 months. The main respiratory disease endpoints and exposure effects were confirmed in both systems, with a greater severity in the NOEC group. Exposure in the WBEC caused generally a more pronounced cardiovascular response. |
| تدمد: | 1099-1263 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8468aa9f3d60e0e95b1f19bc9eb7a800 https://pubmed.ncbi.nlm.nih.gov/33825214 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8468aa9f3d60e0e95b1f19bc9eb7a800 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10991263 |
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