A novel fluorescence and DNA combination for versatile, long‐term marking of mosquitoes
| العنوان: | A novel fluorescence and DNA combination for versatile, long‐term marking of mosquitoes |
|---|---|
| المؤلفون: | Sekou Goita, Moribo Coulibaly, Salif Kone, Olga Muratova, Adama Dao, Tovi Lehmann, Ousmane Yossi, Benjamin J. Krajacich, Bronner P. Gonçalves, Mamadou B. Coulibaly, Roy Faiman, Djibril Samaké, Daman Sylla, Zana L. Sanogo, Moussa Diallo, Ashley McCormack, Alpha S. Yaro, Patrick E. Duffy, Jennifer Hume, Leland Graber |
| المصدر: | Methods in Ecology and Evolution |
| بيانات النشر: | John Wiley and Sons Inc., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Disease Ecology, Anopheles gambiae, chemistry.chemical_compound, Practical Tools, Synthetic DNA, Marking and Tracking, Sequence variation, dispersal, Complex problems, Applied Ecology, Ecology, Evolution, Behavior and Systematics, Mark–release–recapture, fluorescent, biology, Mark release recapture, Life History Ecology, Ecological Modeling, High intensity, DNA, biology.organism_classification, Fluorescence, chemistry, Spatial Ecology, Movement Ecology, Population Ecology, Biological system, Entomology |
| الوصف: | Current mark–release–recapture methodologies are limited in their ability to address complex problems in vector biology, such as studying multiple groups overlapping in space and time. Additionally, limited mark retention, reduced post‐marking survival and the large effort in marking, collection and recapture all complicate effective insect tracking.We have developed and evaluated a marking method using a fluorescent dye (SmartWater®) combined with synthetic DNA tags to informatively and efficiently mark adult mosquitoes using an airbrush pump and nebulizer. Using a handheld UV flashlight, the fluorescent marking enabled quick and simple initial detection of recaptures in a field‐ready and non‐destructive approach that when combined with an extraction‐free PCR on individual mosquito legs provides potentially unlimited marking information.This marking, first tested in the laboratory with Anopheles gambiae s.l. mosquitoes, did not affect survival (median ages 24–28 days, p‐adj > 0.25), oviposition (median eggs/female of 28.8, 32.5, 33.3 for water, green, red dyes, respectively, p‐adj > 0.44) or Plasmodium competence (mean oocysts 5.56–10.6, p‐adj > 0.95). DNA and fluorescence had 100% retention up to 3 weeks (longest time point tested) with high intensity, indicating marks would persist longer.We describe a novel, simple, no/low‐impact and long‐lasting marking method that allows separation of multiple insect subpopulations by combining unlimited length and sequence variation in the synthetic DNA tags. This method can be readily deployed in the field for marking multiple groups of mosquitoes or other insects. |
| اللغة: | English |
| تدمد: | 2041-210X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81f03d541f5930b78c9ae525776b996d http://europepmc.org/articles/PMC8252004 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....81f03d541f5930b78c9ae525776b996d |
| قاعدة البيانات: | OpenAIRE |
كن أول من يترك تعليقا!