Somatostatin and its receptors: functional regulation in the development of mice Sertoli cells
| العنوان: | Somatostatin and its receptors: functional regulation in the development of mice Sertoli cells |
|---|---|
| المؤلفون: | Aixin Liang, Muhammad Shahzad, Hasan Riaz, Muhammad Kasib Khan, Li Han, Zhenlu Chong, Guohua Hua, Ping Dong, Liguo Yang, Sibtain Ahmad |
| المصدر: | The Journal of steroid biochemistry and molecular biology. 138 |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, endocrine system, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Blotting, Western, Apoptosis, Biology, Real-Time Polymerase Chain Reaction, Biochemistry, Mice, Endocrinology, Internal medicine, medicine, Somatostatin receptor 3, Somatostatin receptor 2, Animals, Somatostatin receptor 1, Receptors, Somatostatin, Receptor, Molecular Biology, Cells, Cultured, Sertoli Cells, Somatostatin receptor, Cell Cycle, Cell Biology, Cell cycle, Sertoli cell, Immunohistochemistry, medicine.anatomical_structure, Somatostatin, Molecular Medicine |
| الوصف: | Recently, Sertoli cells have been ascertained as the target for the regulatory peptide somatostatin (SST). Therefore, the present study investigated the expression of somatostatin receptors, their age-related alterations and homologous regulation by in vitro treatment with SRIF14 on mice Sertoli cells; furthermore, it dealt with SRIF14 action on growth progression, apoptotic activity and related gene expressions in these cells. We found that mice Sertoli cells expressed all SST1-5 receptors with differential intensities. Age-related real-time PCR of all somatostatin receptors identified abundance of SSTR2 and SSTR5 mRNA level during Sertoli cell developmental period. Furthermore, higher level of these two receptors was independent of SRIF14, as treatment with SRIF14 failed to induce both receptor expressions when compared with control. Somatostatin treatment elicited a dose-dependent decrease in forskolin stimulated cAMP production. Low (100pM and 10nM) and high dosage (1μM) groups of SRIF14 significantly promoted apoptosis, while all treatment groups led to dose dependent cessation (P |
| تدمد: | 1879-1220 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80a2866431460564dbd0a4e6a2d0224f https://pubmed.ncbi.nlm.nih.gov/23831358 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....80a2866431460564dbd0a4e6a2d0224f |
| قاعدة البيانات: | OpenAIRE |
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