Efforts To Expand the Genetic Alphabet: Identification of a Replicable Unnatural DNA Self-Pair
| العنوان: | Efforts To Expand the Genetic Alphabet: Identification of a Replicable Unnatural DNA Self-Pair |
|---|---|
| المؤلفون: | Allison A. Henry, Bernhard H. Geierstanger, Anne Goldbech Olsen, Floyd E. Romesberg, Chengzhi Yu, Shigeo Matsuda |
| المصدر: | Journal of the American Chemical Society. 126:6923-6931 |
| بيانات النشر: | American Chemical Society (ACS), 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | DNA Replication, Circular dichroism, Magnetic Resonance Spectroscopy, Stereochemistry, Base pair, DNA-Directed DNA Polymerase, Biochemistry, Catalysis, Nucleobase, chemistry.chemical_compound, Colloid and Surface Chemistry, Polyphosphates, Nucleotide, Base Pairing, chemistry.chemical_classification, Base Sequence, Molecular Structure, Circular Dichroism, DNA replication, DNA, Templates, Genetic, General Chemistry, Genetic translation, Template, chemistry, Thermodynamics |
| الوصف: | Six unnatural nucleotides featuring fluorine-substituted phenyl nucleobase analogues have been synthesized, incorporated into DNA, and characterized in terms of the structure and replication properties of the self-pairs they form. Each unnatural self-pair is accommodated in B-form DNA without detectable structural perturbation, and all are thermally stable and selective to roughly the same degree. Furthermore, the efficiency of polymerase-mediated mispair synthesis is similar for each unnatural nucleotide in the template. In contrast, the efficiency of polymerase-mediated self-pair extension is highly dependent on the specific fluorine substitution pattern. The most promising unnatural base pair candidate of this series is the 3-fluorobenzene self-pair, which is replicated with reasonable efficiency and selectivity. |
| تدمد: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja049961u |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::780cdc0da3ffaf243eb83f6e5d5c930a https://doi.org/10.1021/ja049961u |
| رقم الانضمام: | edsair.doi.dedup.....780cdc0da3ffaf243eb83f6e5d5c930a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205126 00027863 |
|---|---|
| DOI: | 10.1021/ja049961u |