Changes in OCT4 expression play a crucial role in the lineage specification and proliferation of preimplantation porcine blastocysts
| العنوان: | Changes in OCT4 expression play a crucial role in the lineage specification and proliferation of preimplantation porcine blastocysts |
|---|---|
| المؤلفون: | Mingyun Lee, Jong‐Nam Oh, Gyung Cheol Choe, Seung‐Hun Kim, Kwang‐Hwan Choi, Dong‐Kyung Lee, Jinsol Jeong, Chang‐Kyu Lee |
| المصدر: | Cell proliferation. 55(11) |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Mammals, Blastocyst, Pregnancy, Swine, Animals, Gene Expression Regulation, Developmental, Female, Cell Differentiation, Cell Biology, General Medicine, Octamer Transcription Factor-3, Cell Proliferation |
| الوصف: | Curiosity about the role of OCT4, a core transcription factor that maintains inner cell mass (ICM) formation during preimplantation embryogenesis and the pluripotent state in embryonic development, has long been an issue. OCT4 has a species-specific expression pattern in mammalian preimplantation embryogenesis and is known to play an essential role in ICM formation. However, there is a need to study new roles for OCT4-related pluripotency networks and second-cell fate decisions.To determine the functions of OCT4 in lineage specification and embryo proliferation, loss- and gain-of-function studies were performed on porcine parthenotes using microinjection. Then, we performed immunocytochemistry and quantitative real-time polymerase chain reaction (PCR) to examine the association of OCT4 with other lineage markers and its effect on downstream genes.In OCT4-targeted late blastocysts, SOX2, NANOG, and SOX17 positive cells were decreased, and the total cell number of blastocysts was also decreased. According to real-time PCR analysis, NANOG, SOX17, and CDK4 were decreased in OCT4-targeted blastocysts, but trophoblast-related genes were increased. In OCT4-overexpressing blastocysts, SOX2 and NANOG positive cells increased, while SOX17 positive cells decreased, and while total cell number of blastocysts increased. As a result of real-time PCR analysis, the expression of SOX2, NANOG, and CDK4 was increased, but the expression of SOX17 was decreased.Taken together, our results demonstrated that OCT4 leads pluripotency in porcine blastocysts and also plays an important role in ICM formation, secondary cell fate decision, and cell proliferation. |
| تدمد: | 1365-2184 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::780533d998cd3ae6986a58483f111b95 https://pubmed.ncbi.nlm.nih.gov/35883229 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....780533d998cd3ae6986a58483f111b95 |
| قاعدة البيانات: | OpenAIRE |
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