New strategy for mapping the human genome based on a novel procedure for construction of jumping libraries
| العنوان: | New strategy for mapping the human genome based on a novel procedure for construction of jumping libraries |
|---|---|
| المؤلفون: | Vladimir I. Kashuba, Eugene R. Zabarovsky, Ferenc Boldog, Eric Stanbridge, János Sümegi, George Klein, Rikard Erlandsson, Gösta Winberg, Lev L. Kisselev, Zoltan Marcsek, Rando L. Allikmets |
| المصدر: | Genomics. 11:1030-1039 |
| بيانات النشر: | Elsevier BV, 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | Genetics, Genome, Human, Genetic Vectors, Chromosome Mapping, Jumping library, Hybrid Cells, Biology, Genome, Electrophoresis, Gel, Pulsed-Field, Sequence-tagged site, Mice, Tumor Cells, Cultured, Primer walking, Animals, Humans, Genes, Tumor Suppressor, Human genome, Genomic library, Chromosomes, Human, Pair 3, Cloning, Molecular, Deoxyribonucleases, Type II Site-Specific, Selectable marker, Gene Library, Genomic organization |
| الوصف: | A novel procedure for construction of jumping libraries is described. The essential features of this procedure are as follows: (1) two diphasmid vectors (lambda SK17 and lambda SK22) are simultaneously used in the library construction to improve representativity, (2) a partial filling-in reaction is used to eliminate cloning of artifactual jumping clones and to obviate the need for a selectable marker. The procedure has been used to construct a representative human NotI jumping library (220,000 independent recombinant clones) from the lymphoblastoid cell line CBMI-Ral-STO, which features a low level of methylation of its resident EBV genomes. A human chromosome 3-specific NotI jumping library (500,000 independent recombinant clones) from the human chromosome 3 x mouse hybrid cell line MCH 903.1 has also been constructed. Of these recombinant clones 50-80% represent jumps to the neighboring cleavable NotI site. With our previously published method for construction of linking libraries this procedure makes a new genome mapping strategy feasible. This strategy includes the determination of tagging sequences adjacent to NotI sites in random linking and jumping clones. Special features of the lambda SK17 and lambda SK22 vectors facilitate such sequencing. The STS (sequence tagged site) information obtained can be assembled by computer into a map representing the linear order of the NotI sites for a chromosome or for the entire genome. The computerized mapping data can be used to retrieve clones near a region of interest. The corresponding clones can be obtained from the panel of original clones, or necessary probes can be made from genomic DNA by PCR. |
| تدمد: | 0888-7543 |
| DOI: | 10.1016/0888-7543(91)90029-e |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::774d928e8d35967ca31264e4611901a1 https://doi.org/10.1016/0888-7543(91)90029-e |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....774d928e8d35967ca31264e4611901a1 |
| قاعدة البيانات: | OpenAIRE |
| ResultId |
1 |
|---|---|
| Header |
edsair OpenAIRE edsair.doi.dedup.....774d928e8d35967ca31264e4611901a1 767 3 unknown 766.625915527344 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsair&AN=edsair.doi.dedup.....774d928e8d35967ca31264e4611901a1&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://explore.openaire.eu/search/publication?articleId=doi_dedup___::774d928e8d35967ca31264e4611901a1# [Name] => EDS - OpenAIRE [Category] => fullText [Text] => View record in OpenAIRE [MouseOverText] => View record in OpenAIRE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => New strategy for mapping the human genome based on a novel procedure for construction of jumping libraries
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Vladimir+I%2E+Kashuba%22">Vladimir I. Kashuba</searchLink><br /><searchLink fieldCode="AR" term="%22Eugene+R%2E+Zabarovsky%22">Eugene R. Zabarovsky</searchLink><br /><searchLink fieldCode="AR" term="%22Ferenc+Boldog%22">Ferenc Boldog</searchLink><br /><searchLink fieldCode="AR" term="%22Eric+Stanbridge%22">Eric Stanbridge</searchLink><br /><searchLink fieldCode="AR" term="%22János+Sümegi%22">János Sümegi</searchLink><br /><searchLink fieldCode="AR" term="%22George+Klein%22">George Klein</searchLink><br /><searchLink fieldCode="AR" term="%22Rikard+Erlandsson%22">Rikard Erlandsson</searchLink><br /><searchLink fieldCode="AR" term="%22Gösta+Winberg%22">Gösta Winberg</searchLink><br /><searchLink fieldCode="AR" term="%22Lev+L%2E+Kisselev%22">Lev L. Kisselev</searchLink><br /><searchLink fieldCode="AR" term="%22Zoltan+Marcsek%22">Zoltan Marcsek</searchLink><br /><searchLink fieldCode="AR" term="%22Rando+L%2E+Allikmets%22">Rando L. Allikmets</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => <i>Genomics</i>. 11:1030-1039 ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Elsevier BV, 1991. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 1991 ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Genetics%22">Genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Genome%2C+Human%22">Genome, Human</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+Vectors%22">Genetic Vectors</searchLink><br /><searchLink fieldCode="DE" term="%22Chromosome+Mapping%22">Chromosome Mapping</searchLink><br /><searchLink fieldCode="DE" term="%22Jumping+library%22">Jumping library</searchLink><br /><searchLink fieldCode="DE" term="%22Hybrid+Cells%22">Hybrid Cells</searchLink><br /><searchLink fieldCode="DE" term="%22Biology%22">Biology</searchLink><br /><searchLink fieldCode="DE" term="%22Genome%22">Genome</searchLink><br /><searchLink fieldCode="DE" term="%22Electrophoresis%2C+Gel%2C+Pulsed-Field%22">Electrophoresis, Gel, Pulsed-Field</searchLink><br /><searchLink fieldCode="DE" term="%22Sequence-tagged+site%22">Sequence-tagged site</searchLink><br /><searchLink fieldCode="DE" term="%22Mice%22">Mice</searchLink><br /><searchLink fieldCode="DE" term="%22Tumor+Cells%2C+Cultured%22">Tumor Cells, Cultured</searchLink><br /><searchLink fieldCode="DE" term="%22Primer+walking%22">Primer walking</searchLink><br /><searchLink fieldCode="DE" term="%22Animals%22">Animals</searchLink><br /><searchLink fieldCode="DE" term="%22Humans%22">Humans</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%2C+Tumor+Suppressor%22">Genes, Tumor Suppressor</searchLink><br /><searchLink fieldCode="DE" term="%22Human+genome%22">Human genome</searchLink><br /><searchLink fieldCode="DE" term="%22Genomic+library%22">Genomic library</searchLink><br /><searchLink fieldCode="DE" term="%22Chromosomes%2C+Human%2C+Pair+3%22">Chromosomes, Human, Pair 3</searchLink><br /><searchLink fieldCode="DE" term="%22Cloning%2C+Molecular%22">Cloning, Molecular</searchLink><br /><searchLink fieldCode="DE" term="%22Deoxyribonucleases%2C+Type+II+Site-Specific%22">Deoxyribonucleases, Type II Site-Specific</searchLink><br /><searchLink fieldCode="DE" term="%22Selectable+marker%22">Selectable marker</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+Library%22">Gene Library</searchLink><br /><searchLink fieldCode="DE" term="%22Genomic+organization%22">Genomic organization</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => A novel procedure for construction of jumping libraries is described. The essential features of this procedure are as follows: (1) two diphasmid vectors (lambda SK17 and lambda SK22) are simultaneously used in the library construction to improve representativity, (2) a partial filling-in reaction is used to eliminate cloning of artifactual jumping clones and to obviate the need for a selectable marker. The procedure has been used to construct a representative human NotI jumping library (220,000 independent recombinant clones) from the lymphoblastoid cell line CBMI-Ral-STO, which features a low level of methylation of its resident EBV genomes. A human chromosome 3-specific NotI jumping library (500,000 independent recombinant clones) from the human chromosome 3 x mouse hybrid cell line MCH 903.1 has also been constructed. Of these recombinant clones 50-80% represent jumps to the neighboring cleavable NotI site. With our previously published method for construction of linking libraries this procedure makes a new genome mapping strategy feasible. This strategy includes the determination of tagging sequences adjacent to NotI sites in random linking and jumping clones. Special features of the lambda SK17 and lambda SK22 vectors facilitate such sequencing. The STS (sequence tagged site) information obtained can be assembled by computer into a map representing the linear order of the NotI sites for a chromosome or for the entire genome. The computerized mapping data can be used to retrieve clones near a region of interest. The corresponding clones can be obtained from the panel of original clones, or necessary probes can be made from genomic DNA by PCR. ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 0888-7543 ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1016/0888-7543(91)90029-e ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://explore.openaire.eu/search/publication?articleId=doi_dedup___::774d928e8d35967ca31264e4611901a1" linkWindow="_blank">https://explore.openaire.eu/search/publication?articleId=doi_dedup___::774d928e8d35967ca31264e4611901a1</link><br /><link linkTarget="URL" linkTerm="https://doi.org/10.1016/0888-7543(91)90029-e" linkWindow="_blank">https://doi.org/10.1016/0888-7543(91)90029-e</link> ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => OPEN ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsair.doi.dedup.....774d928e8d35967ca31264e4611901a1 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1016/0888-7543(91)90029-e
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => Undetermined
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 10
[StartPage] => 1030
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => Genetics
[Type] => general
)
[1] => Array
(
[SubjectFull] => Genome, Human
[Type] => general
)
[2] => Array
(
[SubjectFull] => Genetic Vectors
[Type] => general
)
[3] => Array
(
[SubjectFull] => Chromosome Mapping
[Type] => general
)
[4] => Array
(
[SubjectFull] => Jumping library
[Type] => general
)
[5] => Array
(
[SubjectFull] => Hybrid Cells
[Type] => general
)
[6] => Array
(
[SubjectFull] => Biology
[Type] => general
)
[7] => Array
(
[SubjectFull] => Genome
[Type] => general
)
[8] => Array
(
[SubjectFull] => Electrophoresis, Gel, Pulsed-Field
[Type] => general
)
[9] => Array
(
[SubjectFull] => Sequence-tagged site
[Type] => general
)
[10] => Array
(
[SubjectFull] => Mice
[Type] => general
)
[11] => Array
(
[SubjectFull] => Tumor Cells, Cultured
[Type] => general
)
[12] => Array
(
[SubjectFull] => Primer walking
[Type] => general
)
[13] => Array
(
[SubjectFull] => Animals
[Type] => general
)
[14] => Array
(
[SubjectFull] => Humans
[Type] => general
)
[15] => Array
(
[SubjectFull] => Genes, Tumor Suppressor
[Type] => general
)
[16] => Array
(
[SubjectFull] => Human genome
[Type] => general
)
[17] => Array
(
[SubjectFull] => Genomic library
[Type] => general
)
[18] => Array
(
[SubjectFull] => Chromosomes, Human, Pair 3
[Type] => general
)
[19] => Array
(
[SubjectFull] => Cloning, Molecular
[Type] => general
)
[20] => Array
(
[SubjectFull] => Deoxyribonucleases, Type II Site-Specific
[Type] => general
)
[21] => Array
(
[SubjectFull] => Selectable marker
[Type] => general
)
[22] => Array
(
[SubjectFull] => Gene Library
[Type] => general
)
[23] => Array
(
[SubjectFull] => Genomic organization
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => New strategy for mapping the human genome based on a novel procedure for construction of jumping libraries
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Vladimir I. Kashuba
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Eugene R. Zabarovsky
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ferenc Boldog
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Eric Stanbridge
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => János Sümegi
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => George Klein
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Rikard Erlandsson
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Gösta Winberg
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Lev L. Kisselev
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Zoltan Marcsek
)
)
)
[10] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Rando L. Allikmets
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 12
[Type] => published
[Y] => 1991
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 08887543
)
[1] => Array
(
[Type] => issn-locals
[Value] => edsair
)
[2] => Array
(
[Type] => issn-locals
[Value] => edsairFT
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 11
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Genomics
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |