CD-340 Functionalized Doxorubicin-Loaded Nanoparticle Induces Apoptosis And Reduces Tumor Volume Along With Drug-Related Cardiotoxicity In Mice [Corrigendum]
| العنوان: | CD-340 Functionalized Doxorubicin-Loaded Nanoparticle Induces Apoptosis And Reduces Tumor Volume Along With Drug-Related Cardiotoxicity In Mice [Corrigendum] |
|---|---|
| المؤلفون: | Laboni Mondal, Biswajit Mukherjee, Kaushik Das, Sanchari Bhattacharya, Debasmita Dutta, Shreyasi Chakraborty, Murari Mohan Pal, Raghuvir H Gaonkar, Mita Chatterjee Debnath |
| المصدر: | International Journal of Nanomedicine |
| بيانات النشر: | Dove Press, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | tumor, Organic Chemistry, technology, industry, and agriculture, Biophysics, Pharmaceutical Science, Bioengineering, General Medicine, ligand, Biomaterials, breast cancer, International Journal of Nanomedicine, Drug Discovery, nanoparticles, Corrigendum, targeting, Original Research |
| الوصف: | Background and objective Targeted drug delivery of nanoparticles decorated with site-specific recognition ligands is of considerable interest to minimize cytotoxicity of chemotherapeutics in the normal cells. The study was designed to develop CD-340 antibody-conjugated polylactic-co-glycolic acid (PLGA) nanoparticles loaded with a highly water-soluble potent anticancer drug, doxorubicin (DOX), to specifically deliver entrapped DOX to breast cancer cells. Methods The study showed how to incorporate water-soluble drug in a hydrophobic PLGA (85:15) based matrix which otherwise shows poor drug loading due to leaching effect. The optimized formulation was covalently conjugated to anti-human epidermal growth factor receptor-2 (HER2) antibody (CD-340). Surface conjugation of the ligand was assessed by flow cytometry, confocal microscopy, and gel electrophoresis. Selectivity and cytotoxicity of the experimental nanoparticles were tested on human breast cancer cells SKBR-3, MCF-7, and MDA-MB-231. Both CD-340-conjugated and unconjugated nanoparticles were undergone in vitro and in vivo characterization. Result Higher level of incorporation of DOX (8.5% W/W), which otherwise shows poor drug loading due to leaching effect of the highly water-soluble drug, was seen in this method. In HER2-overexpressing tumor xenograft model, radiolabeled antibody-conjugated nanoparticles showed preferentially more of the formulation accumulation in the tumor area when compared to the treatments with the unconjugated one or with the other control groups of mice. The ligand conjugated nanoparticles showed considerable potential in reduction of tumor growth and cardiac toxicity of DOX in mice, a prominent side-effect of the drug. Conclusion In conclusion, CD-340-conjugated PLGA nanoparticles containing DOX preferentially delivered encapsulated drug to the breast cancer cells and in breast tumor and reduced the breast tumor cells by apoptosis. Site-specific delivery of the formulation to neoplastic cells did not affect normal cells and showed a drastic reduction of DOX-related cardiotoxicity. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1178-2013 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7360bdcf9a434857b7b44a83af439c44 https://www.dovepress.com/corrigendum-cd-340-functionalized-doxorubicin-loaded-nanoparticle-indu-peer-reviewed-article-IJN |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7360bdcf9a434857b7b44a83af439c44 |
| قاعدة البيانات: | OpenAIRE |
| ResultId |
1 |
|---|---|
| Header |
edsair OpenAIRE edsair.doi.dedup.....7360bdcf9a434857b7b44a83af439c44 831 3 unknown 830.824645996094 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsair&AN=edsair.doi.dedup.....7360bdcf9a434857b7b44a83af439c44&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7360bdcf9a434857b7b44a83af439c44# [Name] => EDS - OpenAIRE [Category] => fullText [Text] => View record in OpenAIRE [MouseOverText] => View record in OpenAIRE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => CD-340 Functionalized Doxorubicin-Loaded Nanoparticle Induces Apoptosis And Reduces Tumor Volume Along With Drug-Related Cardiotoxicity In Mice [Corrigendum]
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Laboni+Mondal%22">Laboni Mondal</searchLink><br /><searchLink fieldCode="AR" term="%22Biswajit+Mukherjee%22">Biswajit Mukherjee</searchLink><br /><searchLink fieldCode="AR" term="%22Kaushik+Das%22">Kaushik Das</searchLink><br /><searchLink fieldCode="AR" term="%22Sanchari+Bhattacharya%22">Sanchari Bhattacharya</searchLink><br /><searchLink fieldCode="AR" term="%22Debasmita+Dutta%22">Debasmita Dutta</searchLink><br /><searchLink fieldCode="AR" term="%22Shreyasi+Chakraborty%22">Shreyasi Chakraborty</searchLink><br /><searchLink fieldCode="AR" term="%22Murari+Mohan+Pal%22">Murari Mohan Pal</searchLink><br /><searchLink fieldCode="AR" term="%22Raghuvir+H+Gaonkar%22">Raghuvir H Gaonkar</searchLink><br /><searchLink fieldCode="AR" term="%22Mita+Chatterjee+Debnath%22">Mita Chatterjee Debnath</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => International Journal of Nanomedicine ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Dove Press, 2019. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2019 ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22tumor%22">tumor</searchLink><br /><searchLink fieldCode="DE" term="%22Organic+Chemistry%22">Organic Chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22technology%2C+industry%2C+and+agriculture%22">technology, industry, and agriculture</searchLink><br /><searchLink fieldCode="DE" term="%22Biophysics%22">Biophysics</searchLink><br /><searchLink fieldCode="DE" term="%22Pharmaceutical+Science%22">Pharmaceutical Science</searchLink><br /><searchLink fieldCode="DE" term="%22Bioengineering%22">Bioengineering</searchLink><br /><searchLink fieldCode="DE" term="%22General+Medicine%22">General Medicine</searchLink><br /><searchLink fieldCode="DE" term="%22ligand%22">ligand</searchLink><br /><searchLink fieldCode="DE" term="%22Biomaterials%22">Biomaterials</searchLink><br /><searchLink fieldCode="DE" term="%22breast+cancer%22">breast cancer</searchLink><br /><searchLink fieldCode="DE" term="%22International+Journal+of+Nanomedicine%22">International Journal of Nanomedicine</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+Discovery%22">Drug Discovery</searchLink><br /><searchLink fieldCode="DE" term="%22nanoparticles%22">nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Corrigendum%22">Corrigendum</searchLink><br /><searchLink fieldCode="DE" term="%22targeting%22">targeting</searchLink><br /><searchLink fieldCode="DE" term="%22Original+Research%22">Original Research</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Background and objective Targeted drug delivery of nanoparticles decorated with site-specific recognition ligands is of considerable interest to minimize cytotoxicity of chemotherapeutics in the normal cells. The study was designed to develop CD-340 antibody-conjugated polylactic-co-glycolic acid (PLGA) nanoparticles loaded with a highly water-soluble potent anticancer drug, doxorubicin (DOX), to specifically deliver entrapped DOX to breast cancer cells. Methods The study showed how to incorporate water-soluble drug in a hydrophobic PLGA (85:15) based matrix which otherwise shows poor drug loading due to leaching effect. The optimized formulation was covalently conjugated to anti-human epidermal growth factor receptor-2 (HER2) antibody (CD-340). Surface conjugation of the ligand was assessed by flow cytometry, confocal microscopy, and gel electrophoresis. Selectivity and cytotoxicity of the experimental nanoparticles were tested on human breast cancer cells SKBR-3, MCF-7, and MDA-MB-231. Both CD-340-conjugated and unconjugated nanoparticles were undergone in vitro and in vivo characterization. Result Higher level of incorporation of DOX (8.5% W/W), which otherwise shows poor drug loading due to leaching effect of the highly water-soluble drug, was seen in this method. In HER2-overexpressing tumor xenograft model, radiolabeled antibody-conjugated nanoparticles showed preferentially more of the formulation accumulation in the tumor area when compared to the treatments with the unconjugated one or with the other control groups of mice. The ligand conjugated nanoparticles showed considerable potential in reduction of tumor growth and cardiac toxicity of DOX in mice, a prominent side-effect of the drug. Conclusion In conclusion, CD-340-conjugated PLGA nanoparticles containing DOX preferentially delivered encapsulated drug to the breast cancer cells and in breast tumor and reduced the breast tumor cells by apoptosis. Site-specific delivery of the formulation to neoplastic cells did not affect normal cells and showed a drastic reduction of DOX-related cardiotoxicity. ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => text/html ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 1178-2013 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7360bdcf9a434857b7b44a83af439c44" linkWindow="_blank">https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7360bdcf9a434857b7b44a83af439c44</link><br /><link linkTarget="URL" linkTerm="https://www.dovepress.com/corrigendum-cd-340-functionalized-doxorubicin-loaded-nanoparticle-indu-peer-reviewed-article-IJN" linkWindow="_blank">https://www.dovepress.com/corrigendum-cd-340-functionalized-doxorubicin-loaded-nanoparticle-indu-peer-reviewed-article-IJN</link> ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => OPEN ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsair.doi.dedup.....7360bdcf9a434857b7b44a83af439c44 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => tumor
[Type] => general
)
[1] => Array
(
[SubjectFull] => Organic Chemistry
[Type] => general
)
[2] => Array
(
[SubjectFull] => technology, industry, and agriculture
[Type] => general
)
[3] => Array
(
[SubjectFull] => Biophysics
[Type] => general
)
[4] => Array
(
[SubjectFull] => Pharmaceutical Science
[Type] => general
)
[5] => Array
(
[SubjectFull] => Bioengineering
[Type] => general
)
[6] => Array
(
[SubjectFull] => General Medicine
[Type] => general
)
[7] => Array
(
[SubjectFull] => ligand
[Type] => general
)
[8] => Array
(
[SubjectFull] => Biomaterials
[Type] => general
)
[9] => Array
(
[SubjectFull] => breast cancer
[Type] => general
)
[10] => Array
(
[SubjectFull] => International Journal of Nanomedicine
[Type] => general
)
[11] => Array
(
[SubjectFull] => Drug Discovery
[Type] => general
)
[12] => Array
(
[SubjectFull] => nanoparticles
[Type] => general
)
[13] => Array
(
[SubjectFull] => Corrigendum
[Type] => general
)
[14] => Array
(
[SubjectFull] => targeting
[Type] => general
)
[15] => Array
(
[SubjectFull] => Original Research
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => CD-340 Functionalized Doxorubicin-Loaded Nanoparticle Induces Apoptosis And Reduces Tumor Volume Along With Drug-Related Cardiotoxicity In Mice [Corrigendum]
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Laboni Mondal
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Biswajit Mukherjee
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Kaushik Das
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Sanchari Bhattacharya
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Debasmita Dutta
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Shreyasi Chakraborty
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Murari Mohan Pal
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Raghuvir H Gaonkar
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Mita Chatterjee Debnath
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 29
[M] => 11
[Type] => published
[Y] => 2019
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 11782013
)
[1] => Array
(
[Type] => issn-locals
[Value] => edsair
)
[2] => Array
(
[Type] => issn-locals
[Value] => edsairFT
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => International Journal of Nanomedicine
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |