Evaluation of two rapid molecular test systems to establish an algorithm for fast identification of bacterial pathogens from positive blood cultures
| العنوان: | Evaluation of two rapid molecular test systems to establish an algorithm for fast identification of bacterial pathogens from positive blood cultures |
|---|---|
| المؤلفون: | Philipp Oberhettinger, Jan Zieger, Ingo Autenrieth, Matthias Marschal, Silke Peter |
| المصدر: | Eur J Clin Microbiol Infect Dis European Journal of Clinical Microbiology & Infectious Diseases |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Bacteria, Antibiotic resistance, Diagnostic Tests, Routine, 030106 microbiology, Fungi, Correction, Drug Resistance, Microbial, General Medicine, Bloodstream infection, GenMark ePlex®, Sensitivity and Specificity, 03 medical and health sciences, 030104 developmental biology, Infectious Diseases, Rapid identification system, Molecular Diagnostic Techniques, Blood Culture, Sepsis, Humans, Original Article, Molecular identification, Biofire FilmArray®, Algorithms |
| الوصف: | Fast identification of pathogens directly from positive blood cultures is of highest importance to supply an adequate therapy of bloodstream infections (BSI). There are several platforms providing molecular-based identification, detection of antimicrobial resistance genes, or even a full antimicrobial susceptibility testing (AST). Two of such test systems allowing rapid diagnostics were assessed in this study: The Biofire FilmArray® and the Genmark ePlex®, both fully automated test system with a minimum of hands-on time. Overall 137 BSI episodes were included in our study and compared to conventional culture–based reference methods. The FilmArray® is using one catridge including a panel for the most common bacterial and fungal BSI pathogens as well as selected resistance markers. The ePlex® offers three different cartridges for detection of Gram-positives, Gram-negatives, and fungi resulting in a broader panel including also rare pathogens, putative contaminants, and more genetic resistance markers. The FilmArray® and ePlex® were evaluated for all 137 BSI episodes with FilmArray® detecting 119 and ePlex® detecting 128 of these. For targets on the respective panel of the system, the FilmArray® generated a sensitivity of 98.9% with 100% specificity on Gram-positive isolates. The ePlex® system generated a sensitivity of 94.7% and a specificity of 90.7% on Gram-positive isolates. In each case, the two systems performed with 100% sensitivity and specificity for the detection of Gram-negative specimens covered by each panel. In summary, both evaluated test systems showed a satisfying overall performance for fast pathogen identification and are beneficial tools for accelerating blood culture diagnostics of sepsis patients. Electronic supplementary material The online version of this article (10.1007/s10096-020-03828-5) contains supplementary material, which is available to authorized users. |
| تدمد: | 1435-4373 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::580abd8b1157874937e4769db93f529f https://pubmed.ncbi.nlm.nih.gov/32870443 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....580abd8b1157874937e4769db93f529f |
| قاعدة البيانات: | OpenAIRE |
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