DNA binding of a proflavine derivative bearing a platinum hanging residue
| العنوان: | DNA binding of a proflavine derivative bearing a platinum hanging residue |
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| المؤلفون: | Tarita Biver, Igor Vasyl Nikolayenko, Fernando Secco, Alessia Boggioni, Antonio Bianchi, Silvia Biagini, Marcella Venturini |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Models, Molecular, spectroscopy, Organoplatinum Compounds, Base pair, Stereochemistry, chemistry.chemical_element, Protonation, binding mode, metal complexes, Biochemistry, drugs, Inorganic Chemistry, chemistry.chemical_compound, thermodynamics, protonation constants, complex formation, Binding site, DNA binding, Fluorescence spectroscopy, Proflavine, Equilibrium constant, Platinum, Binding Sites, spectral titrations, protonation constants, binding mode, reaction mechanism, Fluorescence spectroscopy, metal complexes, organometallics, complex formation, DNA binding, drugs, fluorescence, spectroscopy, equilibrium constants, reaction mechanisms, nucleic acids, affinity, binding mode, thermodynamics, kinetics, speciation, Chemistry, equilibrium constants, DNA, Binding constant, spectral titrations, Intercalating Agents, Crystallography, reaction mechanisms, nucleic acids, speciation, kinetics, Acridine, affinity, reaction mechanism, fluorescence, organometallics |
| الوصف: | New platinum(II) complex of 3,6-diamine-9-[6,6-bis(2-aminohethyl)-1,6-diaminohexyl]acridine, AzaPt, has been synthesised and characterised. Behaviour of AzaPt in solution (protonation and possible self-aggregation phenomena) has been investigated by spectral methods (absorbance and fluorescence) at I=0.1M and 25°C, and the equilibrium parameters of binding to calf thymus DNA have been established. Two different modes of DNA binding by the complex were detected, which depend on the polymer to dye molar ratio (P/D). At relatively low P/D values the mode was interpreted as binding by the polyamine residue external to the base pairs, while at high P/D values the binding corresponds to intercalation of the proflavine residue. Such interpretation is supported by the observed salt effect on binding and the temperature variation of the binding constants, which allowed estimating the ΔH and ΔS values contributions. Spectrophotometric analysis of the long time range binding revealed that AzaPt is involved in a slow reaction, interpreted as an attack by the platinum ion on the nucleobases. The time constant for such interaction was calculated and found to be the same order of magnitude as for processes responsible for the action of anti-tumour drugs that do covalently bind to polynucleotides. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56503197bf259557ccbfb33ae7168880 http://hdl.handle.net/11568/149758 |
| Rights: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....56503197bf259557ccbfb33ae7168880 |
| قاعدة البيانات: | OpenAIRE |
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