Spatial mapping of VOC exhalation by means of bronchoscopic sampling
| العنوان: | Spatial mapping of VOC exhalation by means of bronchoscopic sampling |
|---|---|
| المؤلفون: | Svend Kamysek, Radost Saß, Patricia Fuchs, Andrea Bier, Markus Trautner, Paul Stoll, Jochen K. Schubert, Wolfram Miekisch |
| المصدر: | Journal of breath research. 14(4) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Male, Partial Pressure, 01 natural sciences, pCO2, Specimen Handling, 03 medical and health sciences, 0302 clinical medicine, Bronchoscopy, Limit of Detection, medicine, Humans, Respiratory system, Lung, Volatile Organic Compounds, Chromatography, medicine.diagnostic_test, Chemistry, 010401 analytical chemistry, Exhalation, Carbon Dioxide, Middle Aged, 0104 chemical sciences, medicine.anatomical_structure, 030228 respiratory system, Breath gas analysis, Breath Tests, Breathing, Room air distribution, Female, Respiratory tract |
| الوصف: | Breath analysis holds promise for non-invasivein vivomonitoring of disease related processes. However, physiological parameters may considerably affect profiles of exhaled volatile organic substances (VOCs). Volatile substances can be released via alveoli, bronchial mucosa or from the upper airways. The aim of this study was the systematic investigation of the influence of different sampling sites in the respiratory tract on VOC concentration profiles by means of a novel experimental setup.After ethical approval, breath samples were collected from 25 patients undergoing bronchoscopy for endobronchial ultrasound or bronchoscopic lung volume reduction from different sites in the airways. All patients had total intravenous anaesthesia under pressure-controlled ventilation. If necessary, respiratory parameters were adjusted to keep PETCO2= 35–45 mm Hg. 30 ml gas were withdrawn at six sampling sites by means of gastight glass syringes: S1 = Room air, S2 = Inspiration, S3 = Endotracheal tube, S4 = Trachea, S5 = Right B6 segment, S6 = Left B6 segment (S4–S6 through the bronchoscope channel). 10 ml were used for VOC analysis, 20 ml for PCO2determination. Samples were preconcentrated by solid-phase micro-extraction (SPME) and analysed by gas chromatography–mass spectrometry (GC-MS). PCO2was determined in a conventional blood gas analyser.Statistically significant differences in substance concentrations for acetone, isoprene, 2-methyl-pentane and n-hexane could be observed between different sampling sites. Increasing substance concentrations were determined for acetone (15.3%), 2-methyl-pentane (11.4%) and n-hexane (19.3%) when passing from distal to proximal sampling sites. In contrast, isoprene concentrations decreased by 9.9% from proximal to more distal sampling sites. Blank bronchoscope measurements did not show any contaminations.Increased substance concentrations in the proximal respiratory tract may be explained through substance excretion from bronchial mucosa while decreased concentrations could result from absorption or reaction processes. Spatial mapping of VOC profiles can provide novel insights into substance specific exhalation kinetics and mechanisms. |
| تدمد: | 1752-7163 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ba65fccd88798adbe07ced6dbe96542 https://pubmed.ncbi.nlm.nih.gov/33021213 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....4ba65fccd88798adbe07ced6dbe96542 |
| قاعدة البيانات: | OpenAIRE |
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