Cellular and Molecular Probing of Intact Human Organs
| العنوان: | Cellular and Molecular Probing of Intact Human Organs |
|---|---|
| المؤلفون: | Martin Warmer, Hongcheng Mai, Mihail Ivilinov Todorov, Oliver Schoppe, Jan Lipfert, Ruiyao Cai, Hanno Steinke, Benno Gesierich, Elisabeth Kemter, Eckhard Wolf, Milagros N. Wong, Ali Ertürk, Zhouyi Rong, Ingo Bechmann, Oliver T. Bruns, Marco Duering, Johannes C. Paetzold, Tobias B. Huber, Shan Zhao, Rami Ai Maskari, Victor G. Puelles, Bjoern H. Menze, Karen Stanic |
| المصدر: | Cell Cell 180, 796-812 (2020) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, Swine, Transgene, Thyroid Gland, Biology, Eye, Kidney, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Mice, 0302 clinical medicine, Deep Learning, Imaging, Three-Dimensional, Limit of Detection, medicine, Animals, Humans, Pancreas, 030304 developmental biology, Aged, 80 and over, 0303 health sciences, Staining and Labeling, Optical Imaging, Brain, Human kidney, Human brain, Middle Aged, Cell biology, medicine.anatomical_structure, Cellular resolution, Female, Human thyroid, 030217 neurology & neurosurgery, Clearance |
| الوصف: | Optical tissue transparency permits scalable cellular and molecular investigation of complex tissues in 3D. Adult human organs are particularly challenging to render transparent because of the accumulation of dense and sturdy molecules in decades-aged tissues. To overcome these challenges, we developed SHANEL, a method based on a new tissue permeabilization approach to clear and label stiff human organs. We used SHANEL to render the intact adult human brain and kidney transparent and perform 3D histology with antibodies and dyes in centimeters-depth. Thereby, we revealed structural details of the intact human eye, human thyroid, human kidney, and transgenic pig pancreas at the cellular resolution. Furthermore, we developed a deep learning pipeline to analyze millions of cells in cleared human brain tissues within hours with standard lab computers. Overall, SHANEL is a robust and unbiased technology to chart the cellular and molecular architecture of large intact mammalian organs. |
| وصف الملف: | application/pdf |
| تدمد: | 1097-4172 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fd249752c90c8f4df7806c0f654b487 https://pubmed.ncbi.nlm.nih.gov/32059778 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3fd249752c90c8f4df7806c0f654b487 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974172 |
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