Silica Nanoparticle Internalization Improves Chemotactic Behaviour of Human Mesenchymal Stem Cells Acting on the SDF1α/CXCR4 Axis
| العنوان: | Silica Nanoparticle Internalization Improves Chemotactic Behaviour of Human Mesenchymal Stem Cells Acting on the SDF1α/CXCR4 Axis |
|---|---|
| المؤلفون: | Emanuela Vitale, Daniela Rossin, Sadia Perveen, Ivana Miletto, Marco Lo Iacono, Raffaella Rastaldo, Claudia Giachino |
| المصدر: | Biomedicines; Volume 10; Issue 2; Pages: 336 |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | human mesenchymal stem cells, regenerative medicine, silica nanoparticles, hMSC homing, SDF1α/CXCR4 axis, tissue injury, autophagy, cell migration, TNFα/TNFRs axis, Medicine (miscellaneous), equipment and supplies, Tissue injury, Silica nanoparticles, General Biochemistry, Genetics and Molecular Biology, Human mesenchymal stem cells, HMSC homing, Regenerative medicine, Autophagy, Cell migration |
| الوصف: | Human mesenchymal stem cell (hMSC)-based therapy is an emerging resource in regenerative medicine. Despite the innate ability of hMSCs to migrate to sites of injury, homing of infused hMSCs to the target tissue is inefficient. It was shown that silica nanoparticles (SiO2-NPs), previously developed to track the stem cells after transplantation, accumulated in lysosomes leading to a transient blockage of the autophagic flux. Since CXCR4 turnover is mainly regulated by autophagy, we tested the effect of SiO2-NPs on chemotactic migration of hMSCs along the SDF1α/CXCR4 axis that plays a pivotal role in directing MSC homing to sites of injury. Our results showed that SiO2-NP internalization augmented CXCR4 surface levels. We demonstrated that SiO2-NP-dependent CXCR4 increase was transient, and it reversed at the same time as lysosomal compartment normalization. Furthermore, the autophagy inhibitor Bafilomycin-A1 reproduced CXCR4 overexpression in control hMSCs confirming the direct effect of the autophagic degradation blockage on CXCR4 expression. Chemotaxis assays showed that SiO2-NPs increased hMSC migration toward SDF1α. In contrast, migration improvement was not observed in TNFα/TNFR axis, due to the proteasome-dependent TNFR regulation. Overall, our findings demonstrated that SiO2-NP internalization increases the chemotactic behaviour of hMSCs acting on the SDF1α/CXCR4 axis, unmasking a high potential to improve hMSC migration to sites of injury and therapeutic efficacy upon cell injection in vivo. |
| وصف الملف: | application/pdf |
| تدمد: | 2227-9059 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cc7605b8e9f2fdf371c510b0c0e2360 https://pubmed.ncbi.nlm.nih.gov/35203545 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3cc7605b8e9f2fdf371c510b0c0e2360 |
| قاعدة البيانات: | OpenAIRE |
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