Cell Density Effects of Frog Skin Bacteria on Their Capacity to Inhibit Growth of the Chytrid Fungus, Batrachochytrium dendrobatidis
| العنوان: | Cell Density Effects of Frog Skin Bacteria on Their Capacity to Inhibit Growth of the Chytrid Fungus, Batrachochytrium dendrobatidis |
|---|---|
| المؤلفون: | Kiyomi Yasumiba, Sara C. Bell, Ross A. Alford |
| المصدر: | Microbial Ecology. 71:124-130 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Amphibian, Antifungal Agents, Ranidae, Serial dilution, Soil Science, Fungus, 010603 evolutionary biology, 01 natural sciences, Microbiology, 03 medical and health sciences, biology.animal, Animals, Chytridiomycosis, Ecology, Evolution, Behavior and Systematics, Skin, Chytridiomycota, Bacteria, Ecology, biology, Australia, biology.organism_classification, Quorum sensing, 030104 developmental biology, Frog Skin |
| الوصف: | Bacterial symbionts on frog skin can reduce the growth of the chytrid fungus Batrachochytrium dendrobatidis (Bd) through production of inhibitory metabolites. Bacteria can be effective at increasing the resistance of amphibians to chytridiomycosis when added to amphibian skin, and isolates can be screened for production of metabolites that inhibit Bd growth in vitro. However, some bacteria use density-dependent mechanism such as quorum sensing to regulate metabolite production. It is therefore important to consider cell density effects when evaluating bacteria as possible candidates for bioaugmentation. The aim of our study was to evaluate how the density of cutaneous bacteria affects their inhibition of Bd growth in vitro. We sampled cutaneous bacteria isolated from three frog species in the tropical rainforests of northern Queensland, Australia, and selected ten isolates that were inhibitory to Bd in standardised pilot trials. We grew each isolate in liquid culture at a range of initial dilutions, sub-sampled each dilution at a series of times during the first 48 h of growth and measured spectrophotometric absorbance values, cell counts and Bd-inhibitory activity of cell-free supernatants at each time point. The challenge assay results clearly demonstrated that the inhibitory effects of most isolates were density dependent, with relatively low variation among isolates in the minimum cell density needed to inhibit Bd growth. We suggest the use of minimum cell densities and fast-growing candidate isolates to maximise bioaugmentation efforts. |
| تدمد: | 1432-184X 0095-3628 |
| DOI: | 10.1007/s00248-015-0701-9 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c200204ea9bcd1281a846ee5b1ba895 https://doi.org/10.1007/s00248-015-0701-9 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....3c200204ea9bcd1281a846ee5b1ba895 |
| قاعدة البيانات: | OpenAIRE |
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