Adaptive laboratory evolution of tolerance to dicarboxylic acids in Saccharomyces cerevisiae
| العنوان: | Adaptive laboratory evolution of tolerance to dicarboxylic acids in Saccharomyces cerevisiae |
|---|---|
| المؤلفون: | Jens Nielsen, Elsayed Tharwat Tolba Mohamed, Carl Malina, Yun Chen, Mohammad S. Radi, Adam M. Feist, Markus J. Herrgård, Yongjun Wei, Rui Pedro Gomes Pereira |
| المصدر: | Metabolic Engineering. 56:130-141 |
| بيانات النشر: | Elsevier BV, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Muconic acid, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae, Bioengineering, Context (language use), Glutaric acid, Applied Microbiology and Biotechnology, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Fungal, Dicarboxylic Acids, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Adipic acid, biology, 030306 microbiology, Glutaconic acid, biology.organism_classification, Dicarboxylic acid, Pimelic acid, chemistry, Biochemistry, Directed Molecular Evolution, Biotechnology |
| الوصف: | Improving the growth phenotypes of microbes in high product concentrations is an essential design objective in the development of robust cell factories. However, the limited knowledge regarding tolerance mechanisms makes rational design of such traits complicated. Here, adaptive laboratory evolution was used to explore the tolerance mechanisms that Saccharomyces cerevisiae can evolve in the presence of inhibiting concentrations of three dicarboxylic acids: glutaric acid, adipic acid and pimelic acid. Whole-genome sequencing of tolerant mutants enabled the discovery of the genetic changes behind tolerance and most mutations could be linked to the up-regulation of multidrug resistance transporters. The amplification of QDR3, in particular, was shown to confer tolerance not only to the three dicarboxylic acids investigated, but also towards muconic acid and glutaconic acid. In addition to increased acid tolerance, QDR3 overexpression also improved the production of muconic acid in the context of a strain engineered for producing this compound. |
| تدمد: | 1096-7176 |
| DOI: | 10.1016/j.ymben.2019.09.008 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3568edca7cb71beba4b3c1fdee66a6e8 https://doi.org/10.1016/j.ymben.2019.09.008 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....3568edca7cb71beba4b3c1fdee66a6e8 |
| قاعدة البيانات: | OpenAIRE |
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