Organ culture of adult human skin; effect of culture temperature
العنوان: | Organ culture of adult human skin; effect of culture temperature |
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المؤلفون: | Motoaki Maeda, Motoo Matsubara, Akifumi Nishimura, Shigetaro Sotomatsu, Hirokazu Yasuno, Michiko Sato |
المصدر: | The Journal of dermatology. 8(4) |
سنة النشر: | 1981 |
مصطلحات موضوعية: | integumentary system, Epidermis (botany), Tissue Preservation, Mitosis, Human skin, Dermatology, General Medicine, Skin Transplantation, Cell cycle, Biology, Organ culture, In vitro, Cell biology, Cell Movement, Labelling, Culture Techniques, Freezing, Humans |
الوصف: | In the organ culture of adult human skin, long-term preservation of cultured epidermis was obtained when the culture temperature was shifted from 37°C to 32°C. The epidermal cell kinetics were compared for both culture temperatures using the findings of mitotic rate, mitotic distribution and autoradiographic labelling index with tritiated-thymidine (3H-TdR). As a result, it appeared that a lowered culture temperature controlled the cell cycle in vitro to maintain long-term, active epidermal proliferation. |
تدمد: | 0385-2407 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22cdbd6705e1e577494bbbaf4077aa10 https://pubmed.ncbi.nlm.nih.gov/7028827 |
Rights: | CLOSED |
رقم الانضمام: | edsair.doi.dedup.....22cdbd6705e1e577494bbbaf4077aa10 |
قاعدة البيانات: | OpenAIRE |
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