Investigation of adipose tissues in Zucker rats using in vivo and ex vivo magnetic resonance spectroscopy
| العنوان: | Investigation of adipose tissues in Zucker rats using in vivo and ex vivo magnetic resonance spectroscopy |
|---|---|
| المؤلفون: | Silvia Fiorini, M. Fontanella, Pasquina Marzola, Andrea Sbarbati, Elisa Mosconi, Diana M. Sima, Sabine Van Huffel |
| المصدر: | Journal of Lipid Research, Vol 52, Iss 2, Pp 330-336 (2011) |
| بيانات النشر: | Elsevier, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Adipose Tissue, White, Adipose tissue, White adipose tissue, QD415-436, oil, Biochemistry, fat, obese Zucker rat, unsaturation, magnetic resonance spectroscopy, Endocrinology, Adipose Tissue, Brown, In vivo, Internal medicine, Brown adipose tissue, medicine, Animals, Research Articles, Triglycerides, Degree of unsaturation, medicine.diagnostic_test, Chemistry, Fatty Acids, Magnetic resonance imaging, Cell Biology, Nuclear magnetic resonance spectroscopy, Lipids, Magnetic Resonance Imaging, Rats, Rats, Zucker, medicine.anatomical_structure, Adipose Tissue, Fatty Acids, Unsaturated, obese Zucker, Ex vivo |
| الوصف: | In vivo single-voxel magnetic resonance spectroscopy (MRS) at 4.7T and ex vivo high-resolution proton magnetic resonance spectroscopy (HR-NMR) at 500 MHz were used to study the composition of adipose tissues in Zucker obese and Zucker lean rats. Lipid composition was characterized by unsaturation and polyunsaturation indexes and mean chain lengths. In vitro experiments were conducted in known mixtures of triglycerides and oils in order to validate the method. To avoid inaccuracies due to partial peak overlapping in MRS, peak quantification was performed after fitting of spectral peaks by using the QUEST algorithm. The intensity of different spectral lines was also corrected for T2 relaxation. Albeit with different sensitivity and accuracy, both techniques revealed that white adipose tissue is characterized by lower unsaturation and polyunsaturation indexes in obese rats compared with controls. HR-NMR revealed similar differences in brown adipose tissue. The present findings confirm the hypothesis that obese and lean Zucker rats have different adipose tissue composition. |
| اللغة: | English |
| تدمد: | 0022-2275 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::205dd22a8d6ea6bb34d0fea02b1da9ce http://www.sciencedirect.com/science/article/pii/S0022227520405280 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....205dd22a8d6ea6bb34d0fea02b1da9ce |
| قاعدة البيانات: | OpenAIRE |
| ResultId |
1 |
|---|---|
| Header |
edsair OpenAIRE edsair.doi.dedup.....205dd22a8d6ea6bb34d0fea02b1da9ce 795 3 unknown 794.667602539063 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsair&AN=edsair.doi.dedup.....205dd22a8d6ea6bb34d0fea02b1da9ce&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://explore.openaire.eu/search/publication?articleId=doi_dedup___::205dd22a8d6ea6bb34d0fea02b1da9ce# [Name] => EDS - OpenAIRE [Category] => fullText [Text] => View record in OpenAIRE [MouseOverText] => View record in OpenAIRE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Investigation of adipose tissues in Zucker rats using in vivo and ex vivo magnetic resonance spectroscopy
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Silvia+Fiorini%22">Silvia Fiorini</searchLink><br /><searchLink fieldCode="AR" term="%22M%2E+Fontanella%22">M. Fontanella</searchLink><br /><searchLink fieldCode="AR" term="%22Pasquina+Marzola%22">Pasquina Marzola</searchLink><br /><searchLink fieldCode="AR" term="%22Andrea+Sbarbati%22">Andrea Sbarbati</searchLink><br /><searchLink fieldCode="AR" term="%22Elisa+Mosconi%22">Elisa Mosconi</searchLink><br /><searchLink fieldCode="AR" term="%22Diana+M%2E+Sima%22">Diana M. Sima</searchLink><br /><searchLink fieldCode="AR" term="%22Sabine+Van+Huffel%22">Sabine Van Huffel</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Journal of Lipid Research, Vol 52, Iss 2, Pp 330-336 (2011) ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Elsevier, 2011. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2011 ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Male%22">Male</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%2Emedical%5Fspecialty%22">medicine.medical_specialty</searchLink><br /><searchLink fieldCode="DE" term="%22Adipose+Tissue%2C+White%22">Adipose Tissue, White</searchLink><br /><searchLink fieldCode="DE" term="%22Adipose+tissue%22">Adipose tissue</searchLink><br /><searchLink fieldCode="DE" term="%22White+adipose+tissue%22">White adipose tissue</searchLink><br /><searchLink fieldCode="DE" term="%22QD415-436%22">QD415-436</searchLink><br /><searchLink fieldCode="DE" term="%22oil%22">oil</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry%22">Biochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22fat%22">fat</searchLink><br /><searchLink fieldCode="DE" term="%22obese+Zucker+rat%22">obese Zucker rat</searchLink><br /><searchLink fieldCode="DE" term="%22unsaturation%22">unsaturation</searchLink><br /><searchLink fieldCode="DE" term="%22magnetic+resonance+spectroscopy%22">magnetic resonance spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Endocrinology%22">Endocrinology</searchLink><br /><searchLink fieldCode="DE" term="%22Adipose+Tissue%2C+Brown%22">Adipose Tissue, Brown</searchLink><br /><searchLink fieldCode="DE" term="%22In+vivo%22">In vivo</searchLink><br /><searchLink fieldCode="DE" term="%22Internal+medicine%22">Internal medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Brown+adipose+tissue%22">Brown adipose tissue</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%22">medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Animals%22">Animals</searchLink><br /><searchLink fieldCode="DE" term="%22Research+Articles%22">Research Articles</searchLink><br /><searchLink fieldCode="DE" term="%22Triglycerides%22">Triglycerides</searchLink><br /><searchLink fieldCode="DE" term="%22Degree+of+unsaturation%22">Degree of unsaturation</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%2Ediagnostic%5Ftest%22">medicine.diagnostic_test</searchLink><br /><searchLink fieldCode="DE" term="%22Chemistry%22">Chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Fatty+Acids%22">Fatty Acids</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+resonance+imaging%22">Magnetic resonance imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+Biology%22">Cell Biology</searchLink><br /><searchLink fieldCode="DE" term="%22Nuclear+magnetic+resonance+spectroscopy%22">Nuclear magnetic resonance spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Lipids%22">Lipids</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+Resonance+Imaging%22">Magnetic Resonance Imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Rats%22">Rats</searchLink><br /><searchLink fieldCode="DE" term="%22Rats%2C+Zucker%22">Rats, Zucker</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%2Eanatomical%5Fstructure%22">medicine.anatomical_structure</searchLink><br /><searchLink fieldCode="DE" term="%22Adipose+Tissue%22">Adipose Tissue</searchLink><br /><searchLink fieldCode="DE" term="%22Fatty+Acids%2C+Unsaturated%22">Fatty Acids, Unsaturated</searchLink><br /><searchLink fieldCode="DE" term="%22obese+Zucker%22">obese Zucker</searchLink><br /><searchLink fieldCode="DE" term="%22Ex+vivo%22">Ex vivo</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => In vivo single-voxel magnetic resonance spectroscopy (MRS) at 4.7T and ex vivo high-resolution proton magnetic resonance spectroscopy (HR-NMR) at 500 MHz were used to study the composition of adipose tissues in Zucker obese and Zucker lean rats. Lipid composition was characterized by unsaturation and polyunsaturation indexes and mean chain lengths. In vitro experiments were conducted in known mixtures of triglycerides and oils in order to validate the method. To avoid inaccuracies due to partial peak overlapping in MRS, peak quantification was performed after fitting of spectral peaks by using the QUEST algorithm. The intensity of different spectral lines was also corrected for T2 relaxation. Albeit with different sensitivity and accuracy, both techniques revealed that white adipose tissue is characterized by lower unsaturation and polyunsaturation indexes in obese rats compared with controls. HR-NMR revealed similar differences in brown adipose tissue. The present findings confirm the hypothesis that obese and lean Zucker rats have different adipose tissue composition. ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 0022-2275 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://explore.openaire.eu/search/publication?articleId=doi_dedup___::205dd22a8d6ea6bb34d0fea02b1da9ce" linkWindow="_blank">https://explore.openaire.eu/search/publication?articleId=doi_dedup___::205dd22a8d6ea6bb34d0fea02b1da9ce</link><br /><link linkTarget="URL" linkTerm="http://www.sciencedirect.com/science/article/pii/S0022227520405280" linkWindow="_blank">http://www.sciencedirect.com/science/article/pii/S0022227520405280</link> ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => OPEN ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsair.doi.dedup.....205dd22a8d6ea6bb34d0fea02b1da9ce ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 7
[StartPage] => 330
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => Male
[Type] => general
)
[1] => Array
(
[SubjectFull] => medicine.medical_specialty
[Type] => general
)
[2] => Array
(
[SubjectFull] => Adipose Tissue, White
[Type] => general
)
[3] => Array
(
[SubjectFull] => Adipose tissue
[Type] => general
)
[4] => Array
(
[SubjectFull] => White adipose tissue
[Type] => general
)
[5] => Array
(
[SubjectFull] => QD415-436
[Type] => general
)
[6] => Array
(
[SubjectFull] => oil
[Type] => general
)
[7] => Array
(
[SubjectFull] => Biochemistry
[Type] => general
)
[8] => Array
(
[SubjectFull] => fat
[Type] => general
)
[9] => Array
(
[SubjectFull] => obese Zucker rat
[Type] => general
)
[10] => Array
(
[SubjectFull] => unsaturation
[Type] => general
)
[11] => Array
(
[SubjectFull] => magnetic resonance spectroscopy
[Type] => general
)
[12] => Array
(
[SubjectFull] => Endocrinology
[Type] => general
)
[13] => Array
(
[SubjectFull] => Adipose Tissue, Brown
[Type] => general
)
[14] => Array
(
[SubjectFull] => In vivo
[Type] => general
)
[15] => Array
(
[SubjectFull] => Internal medicine
[Type] => general
)
[16] => Array
(
[SubjectFull] => Brown adipose tissue
[Type] => general
)
[17] => Array
(
[SubjectFull] => medicine
[Type] => general
)
[18] => Array
(
[SubjectFull] => Animals
[Type] => general
)
[19] => Array
(
[SubjectFull] => Research Articles
[Type] => general
)
[20] => Array
(
[SubjectFull] => Triglycerides
[Type] => general
)
[21] => Array
(
[SubjectFull] => Degree of unsaturation
[Type] => general
)
[22] => Array
(
[SubjectFull] => medicine.diagnostic_test
[Type] => general
)
[23] => Array
(
[SubjectFull] => Chemistry
[Type] => general
)
[24] => Array
(
[SubjectFull] => Fatty Acids
[Type] => general
)
[25] => Array
(
[SubjectFull] => Magnetic resonance imaging
[Type] => general
)
[26] => Array
(
[SubjectFull] => Cell Biology
[Type] => general
)
[27] => Array
(
[SubjectFull] => Nuclear magnetic resonance spectroscopy
[Type] => general
)
[28] => Array
(
[SubjectFull] => Lipids
[Type] => general
)
[29] => Array
(
[SubjectFull] => Magnetic Resonance Imaging
[Type] => general
)
[30] => Array
(
[SubjectFull] => Rats
[Type] => general
)
[31] => Array
(
[SubjectFull] => Rats, Zucker
[Type] => general
)
[32] => Array
(
[SubjectFull] => medicine.anatomical_structure
[Type] => general
)
[33] => Array
(
[SubjectFull] => Adipose Tissue
[Type] => general
)
[34] => Array
(
[SubjectFull] => Fatty Acids, Unsaturated
[Type] => general
)
[35] => Array
(
[SubjectFull] => obese Zucker
[Type] => general
)
[36] => Array
(
[SubjectFull] => Ex vivo
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Investigation of adipose tissues in Zucker rats using in vivo and ex vivo magnetic resonance spectroscopy
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Silvia Fiorini
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => M. Fontanella
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Pasquina Marzola
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Andrea Sbarbati
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Elisa Mosconi
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Diana M. Sima
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Sabine Van Huffel
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 02
[Type] => published
[Y] => 2011
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 00222275
)
[1] => Array
(
[Type] => issn-locals
[Value] => edsair
)
[2] => Array
(
[Type] => issn-locals
[Value] => edsairFT
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 52
)
[1] => Array
(
[Type] => issue
[Value] => 2
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Journal of Lipid Research
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |