Involvement of autophagy in realgar quantum dots (RQDs) inhibition of human endometrial cancer JEC cells
| العنوان: | Involvement of autophagy in realgar quantum dots (RQDs) inhibition of human endometrial cancer JEC cells |
|---|---|
| المؤلفون: | Guo Luo, Jin-Zhu Wu, Huan Wang, Ke Xu, Jie Liu, Yan Xu, Wanling Zhang, Nian Jiang, Sheng-yu Wang, Zhengyun Liu |
| المصدر: | PeerJ, Vol 8, p e9754 (2020) PeerJ |
| بيانات النشر: | PeerJ Inc., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Autophagosome, Programmed cell death, Women’s Health, lcsh:Medicine, General Biochemistry, Genetics and Molecular Biology, Flow cytometry, ATG12, 03 medical and health sciences, 0302 clinical medicine, EGFP-LC3 plasmid transfection, medicine, Autophagy, 030304 developmental biology, 0303 health sciences, medicine.diagnostic_test, Endometrial cancer cells, Chemistry, Cell growth, General Neuroscience, lcsh:R, Cell Biology, General Medicine, Transfection, Cell cycle, Cell biology, Oncology, 030220 oncology & carcinogenesis, Arsenic and RQDS, General Agricultural and Biological Sciences, Transmission electron microscopy |
| الوصف: | Realgar (As4S4) has been used in traditional Chinese medicines for treatment of malignancies. The poor solubility of As4S4 hampered its clinical applications. Realgar quantum dots (RQDs) were developed to overcome these problems. Previous studies revealed that the RQDs were effective against endometrial cancer JEC cells and hepatocarcinoma HepG2 cells via inducing apoptosis.Apoptosis and autophagy are important programmed cell death pathways leading to anticancer effects. This study further examined effects of RQDs on autophagy, focusing on the formation of the autophagosome in JEC cells. CCK8 assay was used to examine cell proliferation. Flow cytometry was used to analyze cell cycle. Transmission electron microscopy (TEM) was used to examine the autophagy, cells were transfected with pEGFP-C3-MAP1LC3B plasmid to examine effects of RQDs on autophagosome via confocal microscope. Autophagy-related proteins were examined by Western blot. RQDs exhibited cytotoxicity in JEC cells in a concentration- and time- dependent manner. RQDs induced G2 and S phase arrest in JEC cells. RQDs significantly induced autophagy, with the double-membrane and autophagosome-like structures by TEM. The diffused distribution of pEGFP-C3-MAP1LC3B green fluorescence were become the punctuate pattern fluorescence after treatment with RQDs in cells transfected with pEGFP-C3-MAP1LC3B plasmid RQDs increased the expression of autophagyregulatory proteins LC3 I/II, Beclin-1, p62 and Atg12 in a concentration-dependent manner, similar to autophagy induced by serum starvation, except for p62, as induction of p62 is a characteristic of arsenic compounds. Taken together, the present study clearly demonstrated that RQDs can induce autophagy in JEC cells as one of mechanisms of anticancer effects, and indicated that RQDs may be developed as an autophagy inducer. |
| اللغة: | English |
| تدمد: | 2167-8359 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b713bfcf3bd898556368cb562b8ddd3 https://peerj.com/articles/9754.pdf |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1b713bfcf3bd898556368cb562b8ddd3 |
| قاعدة البيانات: | OpenAIRE |
| ResultId |
1 |
|---|---|
| Header |
edsair OpenAIRE edsair.doi.dedup.....1b713bfcf3bd898556368cb562b8ddd3 845 3 unknown 844.736022949219 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsair&AN=edsair.doi.dedup.....1b713bfcf3bd898556368cb562b8ddd3&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b713bfcf3bd898556368cb562b8ddd3# [Name] => EDS - OpenAIRE [Category] => fullText [Text] => View record in OpenAIRE [MouseOverText] => View record in OpenAIRE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Involvement of autophagy in realgar quantum dots (RQDs) inhibition of human endometrial cancer JEC cells
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Guo+Luo%22">Guo Luo</searchLink><br /><searchLink fieldCode="AR" term="%22Jin-Zhu+Wu%22">Jin-Zhu Wu</searchLink><br /><searchLink fieldCode="AR" term="%22Huan+Wang%22">Huan Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Ke+Xu%22">Ke Xu</searchLink><br /><searchLink fieldCode="AR" term="%22Jie+Liu%22">Jie Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Yan+Xu%22">Yan Xu</searchLink><br /><searchLink fieldCode="AR" term="%22Wanling+Zhang%22">Wanling Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Nian+Jiang%22">Nian Jiang</searchLink><br /><searchLink fieldCode="AR" term="%22Sheng-yu+Wang%22">Sheng-yu Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Zhengyun+Liu%22">Zhengyun Liu</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => PeerJ, Vol 8, p e9754 (2020)<br />PeerJ ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => PeerJ Inc., 2020. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2020 ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Autophagosome%22">Autophagosome</searchLink><br /><searchLink fieldCode="DE" term="%22Programmed+cell+death%22">Programmed cell death</searchLink><br /><searchLink fieldCode="DE" term="%22Women%27s+Health%22">Women’s Health</searchLink><br /><searchLink fieldCode="DE" term="%22lcsh%3AMedicine%22">lcsh:Medicine</searchLink><br /><searchLink fieldCode="DE" term="%22General+Biochemistry%2C+Genetics+and+Molecular+Biology%22">General Biochemistry, Genetics and Molecular Biology</searchLink><br /><searchLink fieldCode="DE" term="%22Flow+cytometry%22">Flow cytometry</searchLink><br /><searchLink fieldCode="DE" term="%22ATG12%22">ATG12</searchLink><br /><searchLink fieldCode="DE" term="%2203+medical+and+health+sciences%22">03 medical and health sciences</searchLink><br /><searchLink fieldCode="DE" term="%220302+clinical+medicine%22">0302 clinical medicine</searchLink><br /><searchLink fieldCode="DE" term="%22EGFP-LC3+plasmid+transfection%22">EGFP-LC3 plasmid transfection</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%22">medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Autophagy%22">Autophagy</searchLink><br /><searchLink fieldCode="DE" term="%22030304+developmental+biology%22">030304 developmental biology</searchLink><br /><searchLink fieldCode="DE" term="%220303+health+sciences%22">0303 health sciences</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%2Ediagnostic%5Ftest%22">medicine.diagnostic_test</searchLink><br /><searchLink fieldCode="DE" term="%22Endometrial+cancer+cells%22">Endometrial cancer cells</searchLink><br /><searchLink fieldCode="DE" term="%22Chemistry%22">Chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+growth%22">Cell growth</searchLink><br /><searchLink fieldCode="DE" term="%22General+Neuroscience%22">General Neuroscience</searchLink><br /><searchLink fieldCode="DE" term="%22lcsh%3AR%22">lcsh:R</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+Biology%22">Cell Biology</searchLink><br /><searchLink fieldCode="DE" term="%22General+Medicine%22">General Medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Transfection%22">Transfection</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+cycle%22">Cell cycle</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+biology%22">Cell biology</searchLink><br /><searchLink fieldCode="DE" term="%22Oncology%22">Oncology</searchLink><br /><searchLink fieldCode="DE" term="%22030220+oncology+%26+carcinogenesis%22">030220 oncology & carcinogenesis</searchLink><br /><searchLink fieldCode="DE" term="%22Arsenic+and+RQDS%22">Arsenic and RQDS</searchLink><br /><searchLink fieldCode="DE" term="%22General+Agricultural+and+Biological+Sciences%22">General Agricultural and Biological Sciences</searchLink><br /><searchLink fieldCode="DE" term="%22Transmission+electron+microscopy%22">Transmission electron microscopy</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Realgar (As4S4) has been used in traditional Chinese medicines for treatment of malignancies. The poor solubility of As4S4 hampered its clinical applications. Realgar quantum dots (RQDs) were developed to overcome these problems. Previous studies revealed that the RQDs were effective against endometrial cancer JEC cells and hepatocarcinoma HepG2 cells via inducing apoptosis.Apoptosis and autophagy are important programmed cell death pathways leading to anticancer effects. This study further examined effects of RQDs on autophagy, focusing on the formation of the autophagosome in JEC cells. CCK8 assay was used to examine cell proliferation. Flow cytometry was used to analyze cell cycle. Transmission electron microscopy (TEM) was used to examine the autophagy, cells were transfected with pEGFP-C3-MAP1LC3B plasmid to examine effects of RQDs on autophagosome via confocal microscope. Autophagy-related proteins were examined by Western blot. RQDs exhibited cytotoxicity in JEC cells in a concentration- and time- dependent manner. RQDs induced G2 and S phase arrest in JEC cells. RQDs significantly induced autophagy, with the double-membrane and autophagosome-like structures by TEM. The diffused distribution of pEGFP-C3-MAP1LC3B green fluorescence were become the punctuate pattern fluorescence after treatment with RQDs in cells transfected with pEGFP-C3-MAP1LC3B plasmid RQDs increased the expression of autophagyregulatory proteins LC3 I/II, Beclin-1, p62 and Atg12 in a concentration-dependent manner, similar to autophagy induced by serum starvation, except for p62, as induction of p62 is a characteristic of arsenic compounds. Taken together, the present study clearly demonstrated that RQDs can induce autophagy in JEC cells as one of mechanisms of anticancer effects, and indicated that RQDs may be developed as an autophagy inducer. ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 2167-8359 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b713bfcf3bd898556368cb562b8ddd3" linkWindow="_blank">https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b713bfcf3bd898556368cb562b8ddd3</link><br /><link linkTarget="URL" linkTerm="https://peerj.com/articles/9754.pdf" linkWindow="_blank">https://peerj.com/articles/9754.pdf</link> ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => OPEN ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsair.doi.dedup.....1b713bfcf3bd898556368cb562b8ddd3 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => Autophagosome
[Type] => general
)
[1] => Array
(
[SubjectFull] => Programmed cell death
[Type] => general
)
[2] => Array
(
[SubjectFull] => Women’s Health
[Type] => general
)
[3] => Array
(
[SubjectFull] => lcsh:Medicine
[Type] => general
)
[4] => Array
(
[SubjectFull] => General Biochemistry, Genetics and Molecular Biology
[Type] => general
)
[5] => Array
(
[SubjectFull] => Flow cytometry
[Type] => general
)
[6] => Array
(
[SubjectFull] => ATG12
[Type] => general
)
[7] => Array
(
[SubjectFull] => 03 medical and health sciences
[Type] => general
)
[8] => Array
(
[SubjectFull] => 0302 clinical medicine
[Type] => general
)
[9] => Array
(
[SubjectFull] => EGFP-LC3 plasmid transfection
[Type] => general
)
[10] => Array
(
[SubjectFull] => medicine
[Type] => general
)
[11] => Array
(
[SubjectFull] => Autophagy
[Type] => general
)
[12] => Array
(
[SubjectFull] => 030304 developmental biology
[Type] => general
)
[13] => Array
(
[SubjectFull] => 0303 health sciences
[Type] => general
)
[14] => Array
(
[SubjectFull] => medicine.diagnostic_test
[Type] => general
)
[15] => Array
(
[SubjectFull] => Endometrial cancer cells
[Type] => general
)
[16] => Array
(
[SubjectFull] => Chemistry
[Type] => general
)
[17] => Array
(
[SubjectFull] => Cell growth
[Type] => general
)
[18] => Array
(
[SubjectFull] => General Neuroscience
[Type] => general
)
[19] => Array
(
[SubjectFull] => lcsh:R
[Type] => general
)
[20] => Array
(
[SubjectFull] => Cell Biology
[Type] => general
)
[21] => Array
(
[SubjectFull] => General Medicine
[Type] => general
)
[22] => Array
(
[SubjectFull] => Transfection
[Type] => general
)
[23] => Array
(
[SubjectFull] => Cell cycle
[Type] => general
)
[24] => Array
(
[SubjectFull] => Cell biology
[Type] => general
)
[25] => Array
(
[SubjectFull] => Oncology
[Type] => general
)
[26] => Array
(
[SubjectFull] => 030220 oncology & carcinogenesis
[Type] => general
)
[27] => Array
(
[SubjectFull] => Arsenic and RQDS
[Type] => general
)
[28] => Array
(
[SubjectFull] => General Agricultural and Biological Sciences
[Type] => general
)
[29] => Array
(
[SubjectFull] => Transmission electron microscopy
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Involvement of autophagy in realgar quantum dots (RQDs) inhibition of human endometrial cancer JEC cells
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Guo Luo
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Jin-Zhu Wu
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Huan Wang
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ke Xu
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Jie Liu
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Yan Xu
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Wanling Zhang
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Nian Jiang
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Sheng-yu Wang
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Zhengyun Liu
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 10
[Type] => published
[Y] => 2020
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 21678359
)
[1] => Array
(
[Type] => issn-locals
[Value] => edsair
)
[2] => Array
(
[Type] => issn-locals
[Value] => edsairFT
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 8
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => PeerJ
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |