Multiple forms of porcine pancreatic phospholipase A2: Isolation and specificity
| العنوان: | Multiple forms of porcine pancreatic phospholipase A2: Isolation and specificity |
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| المؤلفون: | William R. Snyder, Francis H. C. Tsao, Ferenc J. Kezdy, John H. Law, Herschel Cohen |
| المصدر: | Journal of Supramolecular Structure. 1:490-497 |
| بيانات النشر: | Wiley, 1973. |
| سنة النشر: | 1973 |
| مصطلحات موضوعية: | food.ingredient, Swine, Phospholipase, Chromatography, DEAE-Cellulose, chemistry.chemical_compound, food, Yolk, Animals, Amino Acids, Trichloroacetic acid, Pancreas, chemistry.chemical_classification, Phospholipase A, Chromatography, Elution, Substrate (chemistry), General Medicine, Chromatography, Ion Exchange, Egg Yolk, Isoenzymes, Molecular Weight, Enzyme, Biochemistry, chemistry, Phospholipases, Pancreatin, Emulsion, Chromatography, Gel, Phosphatidylcholines, Female, Spectrophotometry, Ultraviolet, Chromatography, Thin Layer, Ultracentrifugation |
| الوصف: | Porcine pancreatic phospholipase A2 was purified from commercial pancreatin by a method involving heat denaturation, trichloroacetic acid precipitation, and DEAE-cellulose chromatography. Assaying the eluate of the chromatography step by a new titrimetric method using vegetable lecithin-albumin emulsion as the substrate, several species of phospholipase A were found. Some of these went undetected when the conventional egg yolk emulsion assay was used. Two phospholipases A2 were isolated in a homogeneous form and shown to have similar chemical and physical properties. Catalytic specificity of the two enzymes differs remarkably toward lecithins in different emulsified states. |
| تدمد: | 1547-9366 0091-7419 |
| DOI: | 10.1002/jss.400010605 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19314f9b63409324b019bc6fbaff695b https://doi.org/10.1002/jss.400010605 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....19314f9b63409324b019bc6fbaff695b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15479366 00917419 |
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| DOI: | 10.1002/jss.400010605 |