أعرض في EDS

Myosin Heads Are Displaced from Actin Filaments in the In Situ Beating Rat Heart in Early Diabetes

التفاصيل البيبلوغرافية
العنوان: Myosin Heads Are Displaced from Actin Filaments in the In Situ Beating Rat Heart in Early Diabetes
المؤلفون: Naoto Yagi, Yutaka Fujii, Daryl O. Schwenke, Darren J. Kelly, Mathew J. Jenkins, James T. Pearson, Amanda J. Edgley, Hatsue Ishibashi-Ueda, Takashi Sonobe, Mikiyasu Shirai
المصدر: Biophysical Journal. 104:1065-1072
بيانات النشر: Elsevier BV, 2013.
سنة النشر: 2013
مصطلحات موضوعية: Male, medicine.medical_specialty, Diabetic Cardiomyopathies, Biophysics, Cardiomyopathy, Myosins, 030204 cardiovascular system & hematology, Diabetes Mellitus, Experimental, Rats, Sprague-Dawley, 03 medical and health sciences, Myosin head, 0302 clinical medicine, X-Ray Diffraction, Diabetic cardiomyopathy, Internal medicine, Scattering, Small Angle, Myosin, Ventricular Pressure, medicine, Animals, Molecular Machines, Motors, and Nanoscale Biophysics, 030304 developmental biology, 0303 health sciences, business.industry, Cardiac muscle, medicine.disease, Actin cytoskeleton, Myocardial Contraction, Rats, Actin Cytoskeleton, medicine.anatomical_structure, Endocrinology, Ventricular pressure, Dobutamine, business, medicine.drug
الوصف: Diabetes is independently associated with a specific cardiomyopathy, characterized by impaired cardiac muscle relaxation and force development. Using synchrotron radiation small-angle x-ray scattering, this study investigated in the in situ heart and in real-time whether changes in cross-bridge disposition and myosin interfilament spacing underlie the early development of diabetic cardiomyopathy. Experiments were conducted using anesthetized Sprague-Dawley rats 3 weeks after treatment with either vehicle (control) or streptozotocin (diabetic). Diffraction patterns were recorded during baseline and dobutamine infusions simultaneous with ventricular pressure-volumetry. From these diffraction patterns myosin mass transfer to actin filaments was assessed as the change in intensity ratio (I1,0/I1,1). In diabetic hearts cross-bridge disposition was most notably abnormal in the diastolic phase (p < 0.05) and to a lesser extent the systolic phase (p < 0.05). In diabetic rats only, there was a transmural gradient of contractile depression. Elevated diabetic end-diastolic intensity ratios were correlated with the suppression of diastolic function (p < 0.05). Furthermore, the expected increase in myosin head transfer by dobutamine was significantly blunted in diabetic animals (p < 0.05). Interfilament spacing did not differ between groups. We reveal that impaired cross-bridge disposition and radial transfer may thus underlie the early decline in ventricular function observed in diabetic cardiomyopathy.
تدمد: 0006-3495
DOI: 10.1016/j.bpj.2013.01.037
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11554d25c2e8480c230466e9a504a1ee
https://doi.org/10.1016/j.bpj.2013.01.037
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....11554d25c2e8480c230466e9a504a1ee
قاعدة البيانات: OpenAIRE
ResultId 1
Header edsair
OpenAIRE
edsair.doi.dedup.....11554d25c2e8480c230466e9a504a1ee
799
3

unknown
798.915649414063
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsair&AN=edsair.doi.dedup.....11554d25c2e8480c230466e9a504a1ee&custid=s6537998&authtype=sso
FullText Array ( [Availability] => 0 )
Array ( [0] => Array ( [Url] => https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11554d25c2e8480c230466e9a504a1ee# [Name] => EDS - OpenAIRE [Category] => fullText [Text] => View record in OpenAIRE [MouseOverText] => View record in OpenAIRE ) )
Items Array ( [Name] => Title [Label] => Title [Group] => Ti [Data] => Myosin Heads Are Displaced from Actin Filaments in the In Situ Beating Rat Heart in Early Diabetes )
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Naoto+Yagi%22&quot;&gt;Naoto Yagi&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Yutaka+Fujii%22&quot;&gt;Yutaka Fujii&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Daryl+O%2E+Schwenke%22&quot;&gt;Daryl O. Schwenke&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Darren+J%2E+Kelly%22&quot;&gt;Darren J. Kelly&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Mathew+J%2E+Jenkins%22&quot;&gt;Mathew J. Jenkins&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22James+T%2E+Pearson%22&quot;&gt;James T. Pearson&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Amanda+J%2E+Edgley%22&quot;&gt;Amanda J. Edgley&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Hatsue+Ishibashi-Ueda%22&quot;&gt;Hatsue Ishibashi-Ueda&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Takashi+Sonobe%22&quot;&gt;Takashi Sonobe&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Mikiyasu+Shirai%22&quot;&gt;Mikiyasu Shirai&lt;/searchLink&gt; )
Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => &lt;i&gt;Biophysical Journal&lt;/i&gt;. 104:1065-1072 )
Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Elsevier BV, 2013. )
Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2013 )
Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Male%22&quot;&gt;Male&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22medicine%2Emedical%5Fspecialty%22&quot;&gt;medicine.medical_specialty&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Diabetic+Cardiomyopathies%22&quot;&gt;Diabetic Cardiomyopathies&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Biophysics%22&quot;&gt;Biophysics&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Cardiomyopathy%22&quot;&gt;Cardiomyopathy&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Myosins%22&quot;&gt;Myosins&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22030204+cardiovascular+system+%26+hematology%22&quot;&gt;030204 cardiovascular system &amp; hematology&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Diabetes+Mellitus%2C+Experimental%22&quot;&gt;Diabetes Mellitus, Experimental&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Rats%2C+Sprague-Dawley%22&quot;&gt;Rats, Sprague-Dawley&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%2203+medical+and+health+sciences%22&quot;&gt;03 medical and health sciences&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Myosin+head%22&quot;&gt;Myosin head&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%220302+clinical+medicine%22&quot;&gt;0302 clinical medicine&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22X-Ray+Diffraction%22&quot;&gt;X-Ray Diffraction&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Diabetic+cardiomyopathy%22&quot;&gt;Diabetic cardiomyopathy&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Internal+medicine%22&quot;&gt;Internal medicine&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Scattering%2C+Small+Angle%22&quot;&gt;Scattering, Small Angle&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Myosin%22&quot;&gt;Myosin&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Ventricular+Pressure%22&quot;&gt;Ventricular Pressure&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22medicine%22&quot;&gt;medicine&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Animals%22&quot;&gt;Animals&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Molecular+Machines%2C+Motors%2C+and+Nanoscale+Biophysics%22&quot;&gt;Molecular Machines, Motors, and Nanoscale Biophysics&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22030304+developmental+biology%22&quot;&gt;030304 developmental biology&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%220303+health+sciences%22&quot;&gt;0303 health sciences&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22business%2Eindustry%22&quot;&gt;business.industry&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Cardiac+muscle%22&quot;&gt;Cardiac muscle&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22medicine%2Edisease%22&quot;&gt;medicine.disease&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Actin+cytoskeleton%22&quot;&gt;Actin cytoskeleton&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Myocardial+Contraction%22&quot;&gt;Myocardial Contraction&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Rats%22&quot;&gt;Rats&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Actin+Cytoskeleton%22&quot;&gt;Actin Cytoskeleton&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22medicine%2Eanatomical%5Fstructure%22&quot;&gt;medicine.anatomical_structure&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Endocrinology%22&quot;&gt;Endocrinology&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Ventricular+pressure%22&quot;&gt;Ventricular pressure&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Dobutamine%22&quot;&gt;Dobutamine&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22business%22&quot;&gt;business&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22medicine%2Edrug%22&quot;&gt;medicine.drug&lt;/searchLink&gt; )
Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Diabetes is independently associated with a specific cardiomyopathy, characterized by impaired cardiac muscle relaxation and force development. Using synchrotron radiation small-angle x-ray scattering, this study investigated in the in situ heart and in real-time whether changes in cross-bridge disposition and myosin interfilament spacing underlie the early development of diabetic cardiomyopathy. Experiments were conducted using anesthetized Sprague-Dawley rats 3 weeks after treatment with either vehicle (control) or streptozotocin (diabetic). Diffraction patterns were recorded during baseline and dobutamine infusions simultaneous with ventricular pressure-volumetry. From these diffraction patterns myosin mass transfer to actin filaments was assessed as the change in intensity ratio (I1,0/I1,1). In diabetic hearts cross-bridge disposition was most notably abnormal in the diastolic phase (p &lt; 0.05) and to a lesser extent the systolic phase (p &lt; 0.05). In diabetic rats only, there was a transmural gradient of contractile depression. Elevated diabetic end-diastolic intensity ratios were correlated with the suppression of diastolic function (p &lt; 0.05). Furthermore, the expected increase in myosin head transfer by dobutamine was significantly blunted in diabetic animals (p &lt; 0.05). Interfilament spacing did not differ between groups. We reveal that impaired cross-bridge disposition and radial transfer may thus underlie the early decline in ventricular function observed in diabetic cardiomyopathy. )
Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 0006-3495 )
Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1016/j.bpj.2013.01.037 )
Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => &lt;link linkTarget=&quot;URL&quot; linkTerm=&quot;https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11554d25c2e8480c230466e9a504a1ee&quot; linkWindow=&quot;_blank&quot;&gt;https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11554d25c2e8480c230466e9a504a1ee&lt;/link&gt;&lt;br /&gt;&lt;link linkTarget=&quot;URL&quot; linkTerm=&quot;https://doi.org/10.1016/j.bpj.2013.01.037&quot; linkWindow=&quot;_blank&quot;&gt;https://doi.org/10.1016/j.bpj.2013.01.037&lt;/link&gt; )
Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => OPEN )
Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsair.doi.dedup.....11554d25c2e8480c230466e9a504a1ee )
RecordInfo Array ( [BibEntity] => Array ( [Identifiers] => Array ( [0] => Array ( [Type] => doi [Value] => 10.1016/j.bpj.2013.01.037 ) ) [Languages] => Array ( [0] => Array ( [Text] => Undetermined ) ) [PhysicalDescription] => Array ( [Pagination] => Array ( [PageCount] => 8 [StartPage] => 1065 ) ) [Subjects] => Array ( [0] => Array ( [SubjectFull] => Male [Type] => general ) [1] => Array ( [SubjectFull] => medicine.medical_specialty [Type] => general ) [2] => Array ( [SubjectFull] => Diabetic Cardiomyopathies [Type] => general ) [3] => Array ( [SubjectFull] => Biophysics [Type] => general ) [4] => Array ( [SubjectFull] => Cardiomyopathy [Type] => general ) [5] => Array ( [SubjectFull] => Myosins [Type] => general ) [6] => Array ( [SubjectFull] => 030204 cardiovascular system & hematology [Type] => general ) [7] => Array ( [SubjectFull] => Diabetes Mellitus, Experimental [Type] => general ) [8] => Array ( [SubjectFull] => Rats, Sprague-Dawley [Type] => general ) [9] => Array ( [SubjectFull] => 03 medical and health sciences [Type] => general ) [10] => Array ( [SubjectFull] => Myosin head [Type] => general ) [11] => Array ( [SubjectFull] => 0302 clinical medicine [Type] => general ) [12] => Array ( [SubjectFull] => X-Ray Diffraction [Type] => general ) [13] => Array ( [SubjectFull] => Diabetic cardiomyopathy [Type] => general ) [14] => Array ( [SubjectFull] => Internal medicine [Type] => general ) [15] => Array ( [SubjectFull] => Scattering, Small Angle [Type] => general ) [16] => Array ( [SubjectFull] => Myosin [Type] => general ) [17] => Array ( [SubjectFull] => Ventricular Pressure [Type] => general ) [18] => Array ( [SubjectFull] => medicine [Type] => general ) [19] => Array ( [SubjectFull] => Animals [Type] => general ) [20] => Array ( [SubjectFull] => Molecular Machines, Motors, and Nanoscale Biophysics [Type] => general ) [21] => Array ( [SubjectFull] => 030304 developmental biology [Type] => general ) [22] => Array ( [SubjectFull] => 0303 health sciences [Type] => general ) [23] => Array ( [SubjectFull] => business.industry [Type] => general ) [24] => Array ( [SubjectFull] => Cardiac muscle [Type] => general ) [25] => Array ( [SubjectFull] => medicine.disease [Type] => general ) [26] => Array ( [SubjectFull] => Actin cytoskeleton [Type] => general ) [27] => Array ( [SubjectFull] => Myocardial Contraction [Type] => general ) [28] => Array ( [SubjectFull] => Rats [Type] => general ) [29] => Array ( [SubjectFull] => Actin Cytoskeleton [Type] => general ) [30] => Array ( [SubjectFull] => medicine.anatomical_structure [Type] => general ) [31] => Array ( [SubjectFull] => Endocrinology [Type] => general ) [32] => Array ( [SubjectFull] => Ventricular pressure [Type] => general ) [33] => Array ( [SubjectFull] => Dobutamine [Type] => general ) [34] => Array ( [SubjectFull] => business [Type] => general ) [35] => Array ( [SubjectFull] => medicine.drug [Type] => general ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Myosin Heads Are Displaced from Actin Filaments in the In Situ Beating Rat Heart in Early Diabetes [Type] => main ) ) ) [BibRelationships] => Array ( [HasContributorRelationships] => Array ( [0] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Naoto Yagi ) ) ) [1] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Yutaka Fujii ) ) ) [2] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Daryl O. Schwenke ) ) ) [3] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Darren J. Kelly ) ) ) [4] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Mathew J. Jenkins ) ) ) [5] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => James T. Pearson ) ) ) [6] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Amanda J. Edgley ) ) ) [7] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Hatsue Ishibashi-Ueda ) ) ) [8] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Takashi Sonobe ) ) ) [9] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Mikiyasu Shirai ) ) ) ) [IsPartOfRelationships] => Array ( [0] => Array ( [BibEntity] => Array ( [Dates] => Array ( [0] => Array ( [D] => 01 [M] => 03 [Type] => published [Y] => 2013 ) ) [Identifiers] => Array ( [0] => Array ( [Type] => issn-print [Value] => 00063495 ) [1] => Array ( [Type] => issn-locals [Value] => edsair ) [2] => Array ( [Type] => issn-locals [Value] => edsairFT ) ) [Numbering] => Array ( [0] => Array ( [Type] => volume [Value] => 104 ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Biophysical Journal [Type] => main ) ) ) ) ) ) )
IllustrationInfo