Photobleaching setup for the biological end-station of the darmstadt heavy-ion microprobe
| العنوان: | Photobleaching setup for the biological end-station of the darmstadt heavy-ion microprobe |
|---|---|
| المؤلفون: | Bernd E. Fischer, K.-O. Voss, Christina Trautmann, B. Merk, I. Müller, Burkhard Jakob, Marco Durante, Gisela Taucher-Scholz |
| المساهمون: | Merk, B., Voss, K. -O., Muller, I., Fischer, B. E., Jakob, B., Taucher-Scholz, G., Trautmann, C., Durante, M. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Nuclear and High Energy Physics, Microprobe, Microscope, business.industry, Chemistry, Fluorescence recovery after photobleaching, Laser, Photobleaching, law.invention, Numerical aperture, Optics, law, Fluorescence microscope, FRAP, Focal Spot Size, business, Live cell imaging, Instrumentation, Ku80 |
| الوصف: | We report the upgrade of the epifluorescence microscope of the GSI heavy-ion microprobe with a galvo-scanned, 488 nm laser diode. The laser is focussed into the object plane by the water-immersion objective resulting in a focal spot size of about 1 μm. To increase temporal and spatial resolution a water-immersion objective with a high numerical aperture is integrated into the custom-build microscope. The upgraded system can now be used to bleach GFP-tagged proteins recruited to DNA damage induced by targeted single-ion irradiation. The system is demonstrated on NIH 3T3 cells with Ku80-GFP ion-targeted in heterochromatic and euchromatic DNA. Fluorescence recovery after photobleaching (FRAP) is shown to be significantly slower in heterochromatin. © 2013 Elsevier B.V. All rights reserved. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e3dab256d7c953dba7249ba284ee5f9 http://hdl.handle.net/11588/855831 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....0e3dab256d7c953dba7249ba284ee5f9 |
| قاعدة البيانات: | OpenAIRE |
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