RT-PCR identification and typing of astroviruses and Norwalk-like viruses in hospitalized patients with gastroenteritis: evidence of nosocomial infections
| العنوان: | RT-PCR identification and typing of astroviruses and Norwalk-like viruses in hospitalized patients with gastroenteritis: evidence of nosocomial infections |
|---|---|
| المؤلفون: | Stephan S. Monroe, C Chamoux, H Laveran, C Mollat, H Piloquet, O. Traoré, S Billaudel, Gaël Belliot |
| المصدر: | Journal of Clinical Virology. 17:151-158 |
| بيانات النشر: | Elsevier BV, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Serotype, Molecular Sequence Data, medicine.disease_cause, Virus, Microbiology, Feces, Astroviridae Infections, Virology, Rotavirus, medicine, Humans, Typing, Child, Phylogeny, Caliciviridae Infections, Cross Infection, biology, Reverse Transcriptase Polymerase Chain Reaction, Virion, Infant, Sequence Analysis, DNA, Middle Aged, biology.organism_classification, Caliciviridae, Gastroenteritis, Latex fixation test, Microscopy, Electron, Norwalk virus, Infectious Diseases, Child, Preschool, Viral disease, Mamastrovirus |
| الوصف: | Background: Astroviruses (HAstVs) and ‘Norwalk-like viruses' (NLV) are frequent causes of gastroenteritis worldwide, though no data on the strains in circulation or their prevalence is available for France. Objectives: We applied molecular methods to detect HAstVs and NLVs by reverse transcription-polymerase chain reaction (RT-PCR) in fecal samples collected during a 2-year period from children and adults hospitalized with gastroenteritis. Study design: All samples negative for rotavirus and adenovirus by latex agglutination which contained small (25–40 nm) viral particles observed by electron microscopy (EM) were examined by RT-PCR. RT-PCR products were sequenced to characterize the HAstV and NLV strains present. Results: A total of 75 samples were analyzed by RT-PCR, of which 15 were positive for HAstV and 24 for NLV. Several distinct strains of serotype 1 HAstV, the predominant serotype, circulated during the period. Nineteen of the 24 NLVs were of the G2 genogroup including Mexico-like ( n =10), Bristol-like ( n =8), and Hawaii-like viruses ( n =1); two were genogroup 1. Overall, seven (47%) of the 15 HAstV infections and nine (37.5%) of the 24 NLV infections appeared to be nosocomially acquired based on the date of admission in hospital and the date of illness. Conclusion: This study provides additional evidence of the importance of nosocomial infections caused by NLV and HAstV. |
| تدمد: | 1386-6532 |
| DOI: | 10.1016/s1386-6532(00)00088-3 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::091c4ffc7386a2d69b02cf4277362ea3 https://doi.org/10.1016/s1386-6532(00)00088-3 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....091c4ffc7386a2d69b02cf4277362ea3 |
| قاعدة البيانات: | OpenAIRE |
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Objectives: We applied molecular methods to detect HAstVs and NLVs by reverse transcription-polymerase chain reaction (RT-PCR) in fecal samples collected during a 2-year period from children and adults hospitalized with gastroenteritis. Study design: All samples negative for rotavirus and adenovirus by latex agglutination which contained small (25–40 nm) viral particles observed by electron microscopy (EM) were examined by RT-PCR. RT-PCR products were sequenced to characterize the HAstV and NLV strains present. Results: A total of 75 samples were analyzed by RT-PCR, of which 15 were positive for HAstV and 24 for NLV. Several distinct strains of serotype 1 HAstV, the predominant serotype, circulated during the period. Nineteen of the 24 NLVs were of the G2 genogroup including Mexico-like ( n =10), Bristol-like ( n =8), and Hawaii-like viruses ( n =1); two were genogroup 1. 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