Spectrum-effect relationship between UHPLC-Q-TOF/MS fingerprint and promoting gastrointestinal motility activity of Fructus aurantii based on multivariate statistical analysis
| العنوان: | Spectrum-effect relationship between UHPLC-Q-TOF/MS fingerprint and promoting gastrointestinal motility activity of Fructus aurantii based on multivariate statistical analysis |
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| المؤلفون: | Haifang Chen, Wuliang Yang, Minyong Zhong, Yuanfeng Yang, Li-fen Zhou, Jinbin Yuan, Rifa Qiao, Lin Yang, Min Zhang |
| المصدر: | Journal of Ethnopharmacology. 279:114366 |
| بيانات النشر: | Elsevier BV, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Uhplc q tof ms, Active components, Motility, Biology, Mass Spectrometry, Rats, Sprague-Dawley, Mice, 03 medical and health sciences, 0302 clinical medicine, Drug Discovery, Animals, Outbred Strains, Animals, Chromatography, High Pressure Liquid, 030304 developmental biology, Pharmacology, 0303 health sciences, Gastric emptying, OPLS, Traditional medicine, Reproducibility of Results, Rats, Gastric Emptying, 030220 oncology & carcinogenesis, Multivariate Analysis, Analysis of variance, Multivariate statistical, Gastrointestinal Motility, Drugs, Chinese Herbal, Western medicine |
| الوصف: | Ethnopharmacological relevance Nowadays, gastrointestinal motility disorders (GMD) have reduced the quality of people's daily life worldwide, but there is still a lack of effective western medicine treatment. Fructus aurantii (FA), a representative regulating-qi herbal medicine, has been widely used to treat GMD in China for thousands of years, but it is not clear that which specific components contribute to the efficacy. Aim of the study The efficacy differences of various fractions of FA on normal mice and GMD rats were compared, so as to find out the main effective fraction of FA, and to screen the main regulating-qi components based on spectrum-effect relationship and multivariate statistical analysis. Materials and methods The fingerprints of different fractions of FA were established and main compounds were identified with UHPLC-Q-TOF/MS technique. The promoting gastrointestinal motility activities of FA were evaluated by defecation test, gastric emptying and intestinal propulsion test in mice, and further investigated according to the biochemical analysis of 5-HT, SP, MLT, GAS and VIP in GMD rats’ plasma. One-way ANOVA was used to find out the difference of efficacy. The active components were screened through spectrum-effect relationship with PCA-X, Pearson bivariate correlation analysis and OPLS analysis. Conclusions Ethyl acetate fraction is the main active fraction, and nine compounds are the major regulating-qi components. The developed spectrum-effect analysis can be used for the screening of bioactive components in natural products with high accuracy and reliability. |
| تدمد: | 0378-8741 |
| DOI: | 10.1016/j.jep.2021.114366 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0886f4922c098c452c45d43b6d9e1e33 https://doi.org/10.1016/j.jep.2021.114366 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....0886f4922c098c452c45d43b6d9e1e33 |
| قاعدة البيانات: | OpenAIRE |
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