Ion conductance pathways in potato tuber (Solanum tuberosum) inner mitochondrial membrane
| العنوان: | Ion conductance pathways in potato tuber (Solanum tuberosum) inner mitochondrial membrane |
|---|---|
| المؤلفون: | Wieslawa Jarmuszkiewicz, Adam Szewczyk, Karolina Matkovic, Izabela Koszela-Piotrowska |
| المصدر: | Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1807:275-285 |
| بيانات النشر: | Elsevier BV, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Immunoblotting, Lipid Bilayers, Biophysics, 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, Bioenergetics, Mitochondrion, Biochemistry, Membrane Potentials, Potassium channels, chemistry.chemical_compound, Oxygen Consumption, Planar lipid membrane technique, Inner mitochondrial membrane, Lipid bilayer, Ion channel, Solanum tuberosum, Membrane potential, Chemistry, fungi, food and beverages, Cell Biology, Potassium channel, Mitochondria, Electrophysiology, Potassium channel activity, DIDS, Ion channels, Mitochondrial Membranes, Ion Channel Gating |
| الوصف: | Single-ion channel activities were measured after reconstitution of potato tuber mitochondrial inner membranes into planar lipid bilayers. In addition to the recently described large-conductance Ca(2+)-activated potassium channel activity (Koszela-Piotrowska et al., 2009), the following mitochondrial ion conductance pathways were recorded: (i) an ATP-regulated potassium channel (mitoK(ATP) channel) activity with a conductance of 164+/-8pS, (ii) a large-conductance Ca(2+)-insensitive iberiotoxin-sensitive potassium channel activity with a conductance of 312 pS+/-23, and (iii) a chloride 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS)-inhibited channel activity with a conductance of 117 pS+/-4. In isolated non-phosphorylating potato tuber mitochondria, individual and combined potassium channel activities caused significant (up to 14mV) but not collapsing K(+)-influx-induced membrane potential depolarisation. Under phosphorylating conditions, the coupling parameters were unchanged in the presence of high K(+) level, indicating that plant K(+) channels function as energy-dissipating systems that are not able to divert energy from oxidative phosphorylation. A potato tuber K(+) channel that is ATP-, 5-hydroxydecanonic acid-, glybenclamide-inhibited and diazoxide-stimulated caused low cation flux, modestly decreasing membrane potential (up to a few mV) and increasing respiration in non-phosphorylating mitochondria. Immunological analysis with antibodies raised against the mammalian plasma membrane ATP-regulated K(+) channel identified a pore-forming subunit of the Kir-like family in potato tuber mitochondrial inner membrane. These results suggest that a mitoK(ATP) channel similar to that of mammalian mitochondria is present in potato tuber mitochondria. |
| تدمد: | 0005-2728 |
| DOI: | 10.1016/j.bbabio.2010.12.001 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06bb5b6d612702d7726a7f0dca064ae6 https://doi.org/10.1016/j.bbabio.2010.12.001 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....06bb5b6d612702d7726a7f0dca064ae6 |
| قاعدة البيانات: | OpenAIRE |
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In addition to the recently described large-conductance Ca(2+)-activated potassium channel activity (Koszela-Piotrowska et al., 2009), the following mitochondrial ion conductance pathways were recorded: (i) an ATP-regulated potassium channel (mitoK(ATP) channel) activity with a conductance of 164+/-8pS, (ii) a large-conductance Ca(2+)-insensitive iberiotoxin-sensitive potassium channel activity with a conductance of 312 pS+/-23, and (iii) a chloride 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS)-inhibited channel activity with a conductance of 117 pS+/-4. In isolated non-phosphorylating potato tuber mitochondria, individual and combined potassium channel activities caused significant (up to 14mV) but not collapsing K(+)-influx-induced membrane potential depolarisation. 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