Comparison of multiple red cell volume methods performed concurrently in premature infants following allogeneic transfusion
| العنوان: | Comparison of multiple red cell volume methods performed concurrently in premature infants following allogeneic transfusion |
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| المؤلفون: | Gretchen A. Cress, Donald M. Mock, Anna Bogusiewicz, John A. Widness, Robert L. Schmidt, Nell I. Matthews, Demet Nalbant, Prasad Bhandary, Ronald G. Strauss, M. Bridget Zimmerman |
| المصدر: | Pediatric research |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | medicine.medical_specialty, Blood transfusion, Anemia, medicine.medical_treatment, 030204 cardiovascular system & hematology, Red cell volume, Article, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, Erythrocyte volume, 030225 pediatrics, hemic and lymphatic diseases, Fetal hemoglobin, medicine, Humans, Infant, Very Low Birth Weight, Blood Transfusion, Kidd Blood-Group System, Chromatography, High Pressure Liquid, Fetal Hemoglobin, Erythrocyte Volume, medicine.diagnostic_test, business.industry, Infant, Newborn, medicine.disease, Flow Cytometry, Infant newborn, 3. Good health, Surgery, Anesthesia, Pediatrics, Perinatology and Child Health, Regression Analysis, business, Infant, Premature, Allogeneic transfusion |
| الوصف: | Study of the pathophysiology and treatment of anemia of prematurity is facilitated by direct measurement of red cell volume (RCV) utilizing microliter quantities of blood samples. Our objective was to compare concurrent measurements of multiple direct RCV methods in infants.Eighteen preterm infants receiving clinically indicated transfusions had concurrent flow cytometric determinations of RCV and 24-h red blood cell (RBC) recovery based on donor-recipient differences of biotin-labeled RBCs (BioRBCs), Kidd antigen mismatched RBCs, and fetal hemoglobin-positive (HbF(+)) RBCs. High-performance liquid chromatography (HPLC) was also used for measuring HbF and adult hemoglobin protein concentrations for the determination of RCV.Concurrent RCV measurements using BioRBCs (18 and 54 µg/ml), Kidd antigen, and HbF flow cytometry were not statistically different compared with RCVs measured using the reference BioRBC density (6 µg/ml). By contrast, the HbF-HPLC method overestimated RCV by 45% compared with the reference method. All the methods demonstrated 100% 24-h posttransfusion RBC recovery (PTR24).Because BioRBC, Kidd antigen, and fetal hemoglobin (HbF) flow cytometry are safe and accurate methods requiring10 µl of patient blood for determining RCV and PTR24 in preterm infants, they can be useful in clinical and research studies of anemia and other conditions. |
| اللغة: | English |
| تدمد: | 1530-0447 0031-3998 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::028abffadb60308f0252f5e5009bf569 http://europepmc.org/articles/PMC3907947 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....028abffadb60308f0252f5e5009bf569 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15300447 00313998 |
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