Targeting of HIV-1 Nef to the Centrosome: Implications for Antigen Processing
| العنوان: | Targeting of HIV-1 Nef to the Centrosome: Implications for Antigen Processing |
|---|---|
| المؤلفون: | Vashti G. Lacaille, Matthew J. Androlewicz |
| المصدر: | Traffic. 1:884-891 |
| بيانات النشر: | Wiley, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | COS cells, Antigen processing, MHC class I antigen, viruses, Antigen presentation, virus diseases, Cell Biology, Biology, Biochemistry, Fusion protein, Epitope, Cell biology, Nocodazole, chemistry.chemical_compound, chemistry, Structural Biology, Centrosome, Genetics, Molecular Biology |
| الوصف: | To gain a better understanding of the intracellular sites of antigen processing we have looked at the localization of human immunodeficiency virus (HIV)-1 Nef protein by confocal microscopic and biochemical means. We found that ubiquitin (Ub)-Nef fusion proteins were localized to the centrosome in transfected COS-7 cells, and that the colocalization was inhibited by the microtubule-disrupting agent, nocodazole. Interestingly, we found that Ub-Nef trafficking to the centrosome was not dependent upon the metabolic stability of Ub-Nef nor on the inhibition of proteasome activity. We also analyzed the MHC class I antigen processing of a reporter epitope linked to the Ub-Nef fusion proteins and found that Ub-Nef was processed in COS-7 cells. In addition, we show that this processing was inhibited by nocodazole. We suggest that the centrosome may serve as a site of antigen processing in vivo. |
| تدمد: | 1398-9219 |
| DOI: | 10.1034/j.1600-0854.2000.011107.x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::feb0e9a9a65e2ca6f9720e0c7b513e31 https://doi.org/10.1034/j.1600-0854.2000.011107.x |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........feb0e9a9a65e2ca6f9720e0c7b513e31 |
| قاعدة البيانات: | OpenAIRE |
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