التفاصيل البيبلوغرافية
| العنوان: |
EPR monitoring of wound oxygenation as a biomarker of response to gene therapy encoding hCAP-18/LL37 peptide |
| المؤلفون: |
Philippe Levêque, Bernard Gallez, Martin C. Lam, Véronique Préat, Céline M. Desmet, Gaëlle Vandermeulen, Caroline Bouzin |
| المصدر: |
Magnetic Resonance in Medicine. 79:3267-3273 |
| بيانات النشر: |
Wiley, 2017. |
| سنة النشر: |
2017 |
| مصطلحات موضوعية: |
0301 basic medicine, Pathology, medicine.medical_specialty, medicine.diagnostic_test, business.industry, Electroporation, Microangiopathy, Magnetic resonance imaging, Oxygenation, Hypoxia (medical), medicine.disease, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Biomarker (medicine), Medicine, Immunohistochemistry, Radiology, Nuclear Medicine and imaging, medicine.symptom, business, Wound healing, 030217 neurology & neurosurgery |
| الوصف: |
Purpose To investigate the value of electron paramagnetic resonance oximetry to follow oxygenation in wounds treated by a plasmid-encoding host defense peptide hCAP-18/LL37. Methods Flaps were created on diabetic mice (7- or 12-week-old db/db mice) presenting different levels of microangiopathy. The hCAP-18/LL37-encoding plasmids were administered in wounds by electroporation. Low-frequency electron paramagnetic resonance oximetry using lithium phthalocyanine as the oxygen sensor was used to monitor wound oxygenation in flaps during the healing process. Flaps were analyzed by immunohistochemistry to assess hypoxia and cell proliferation. Kinetics of closure was also assessed in excisional skin wounds. Results A reoxygenation of the flap was observed during the healing process in the 7-week-old db/db treated mice, but not in the untreated mice and the 12-week-old mice. Histological studies demonstrated less hypoxic regions and higher proportion of proliferating cells in hCAP-18/LL37-treated flaps in the 7-week-old db/db treated mice compared with untreated mice. Consistently, the kinetics of excisional wound closure was improved by hCAP-18/LL37 treatment in the 7-week-old db/db but not in the 12-week-old mice. Conclusions Oxygenation measured by electron paramagnetic resonance oximetry is a promising biomarker of response to treatments designed to modulate wound oxygenation. Magn Reson Med, 2017. © 2017 International Society for Magnetic Resonance in Medicine. |
| تدمد: |
0740-3194 |
| DOI: |
10.1002/mrm.26956 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::ce388a6745b444b9d13440dfbeffde79
https://doi.org/10.1002/mrm.26956 |
| Rights: |
CLOSED |
| رقم الانضمام: |
edsair.doi...........ce388a6745b444b9d13440dfbeffde79 |
| قاعدة البيانات: |
OpenAIRE |