Development of a new fluorescence decay measurement system using two-dimensional single-photon counting
| العنوان: | Development of a new fluorescence decay measurement system using two-dimensional single-photon counting |
|---|---|
| المؤلفون: | M. Watanabe, W. Cieslik, M. Koishi, T. Takeshita, M. Fujiwara |
| المصدر: | Journal of Photochemistry and Photobiology A: Chemistry. 80:429-432 |
| بيانات النشر: | Elsevier BV, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Physics, Photomultiplier, business.industry, Dynamic range, General Chemical Engineering, Physics::Optics, General Physics and Astronomy, General Chemistry, Fluorescence, Photon counting, Wavelength, Optics, Picosecond, Deconvolution, business, Spectrograph |
| الوصف: | A new fluorescence decay measurement system has been developed. The system consists of a spectrograph and a new two-dimensional photon counter. The combination enables measurements to be made of the fluorescence decay as a function of time and wavelength simultaneously. The time resolution is better than 5 ps with deconvolution processing, and the wavelength resolution is approximately 0.15 nm with 1200 grooves mm−1 gratings. The dynamic range is 105. The instrument response function (IRF) of the system is nearly gaussian, and has no tail or “after pulses” which are commonly observed using a photomultiplier in a time-correlated photon counting (TCPC) system. Therefore fast fluorescence decay of several tens of picoseconds can be measured accurately. In addition, the two-dimensional single-photon counting can be performed without wavelength scanning, so that the wavelength-dependent fluorescence decay can be easily and direcly observed with a fast throughput and a high signal-to-noise ratio. The principle of two-dimensional photon counting is discussed together with characteristics including linearity and statistical behavior. |
| تدمد: | 1010-6030 |
| DOI: | 10.1016/1010-6030(93)01009-q |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::cb7a6035a0beaa8d9ff25a2700c1fa9f https://doi.org/10.1016/1010-6030(93)01009-q |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........cb7a6035a0beaa8d9ff25a2700c1fa9f |
| قاعدة البيانات: | OpenAIRE |
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