Dose-Related Influence of Sodium Selenite on Apoptosis in Human Thyroid Follicles In Vitro Induced by Iodine, EGF, TGF-β, and H2O2
| العنوان: | Dose-Related Influence of Sodium Selenite on Apoptosis in Human Thyroid Follicles In Vitro Induced by Iodine, EGF, TGF-β, and H2O2 |
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| المؤلفون: | Klaus L. J. Hallfeldt, Petra Lehmann, Petra Rank, Roland Gärtner, Bjarne Krebs |
| المصدر: | Biological Trace Element Research. 112:119-130 |
| بيانات النشر: | Springer Science and Business Media LLC, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | chemistry.chemical_classification, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Glutathione peroxidase, Biochemistry (medical), Clinical Biochemistry, Thyroid, chemistry.chemical_element, General Medicine, Iodine, Biochemistry, Inorganic Chemistry, Endocrinology, medicine.anatomical_structure, chemistry, Apoptosis, Epidermal growth factor, Internal medicine, Follicular phase, medicine, Selenium, Hormone |
| الوصف: | Apoptosis of thyroid follicular cells is induced by high doses of iodide, epidermal growth factor (EGF), transforming growth factor-β (TGF-β), as well as H2O2 and might be attenuated by antioxidants. Therefore, we examined the apoptotic index induced by these substances in selenium-treated vs untreated human thyroid follicular cells. Reconstituted human thyroid follicles were incubated with sodium selenite (10 or 100 nM) for 72 h; controls received none. The follicles were then distributed to 24-well plates and incubated with potassium iodide (5, 10, or 20 nM), EGF (5 ng/mL), TGF-β (5 ng/mL), or H2O2 (100 μM). Apoptosis was determined by a mitochondrial potential assay and the number of apoptotic cells counted by two independent, experienced technicians and the glutathione peroxidase (GPx) activity was determined. A significant increase of apoptic cells was obtained in control thyroid follicles treated with iodine (5, 10, or 20 μM), thyroid-stimulating hormone (TSH) 1, or 10 mU/mL in combination with 5 and 10 μM iodine, EGF (5 ng/mL) and TGF-β (5 ng/mL), or H2O2 (100 μM) (p |
| تدمد: | 0163-4984 |
| DOI: | 10.1385/bter:112:2:119 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::8779ac9a246ce7dd6b20e172221d6844 https://doi.org/10.1385/bter:112:2:119 |
| رقم الانضمام: | edsair.doi...........8779ac9a246ce7dd6b20e172221d6844 |
| قاعدة البيانات: | OpenAIRE |
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