The discovery of new inhibitory natural compounds targeting bacterial virulence is an important strategy to overcome resistance to traditional antibiotics, in the present study the antibacterial activity of micro and nano curcumin suspensions was investigated against four predominant bacterial pathogens, including E. coli, P. aeruginosa, S. aureus, and B. subtilus. In vitro antibacterial assay indicated that nanocurcumin suspension displayed significant results. Molecular docking analysis was conducted to confirm the impact of curcumin on the quorum-sensing pathway in all bacterial groups, also the pharmacokinetic properties revealed that curcumin can be classified as a drug like compound. Wound model was employed for wound healing activity in four equal groups of male albino rats, their dorsal thoracic central regions were wounded with surgical blades. The individual groups were treated daily with 5% W/W of micro and nano curcumin lotions and soframycin ointment. Topical application of nanocurcumin lotion showed marked reduction on wound area compared to the control group as wound healing reached 98.8% on the 15th of injury. ANOVA test was performed to assess the significance of mean differences. The findings from this research indicate that nanocurcumin is effective in inhibiting bacterial infections and faster the process of wound healing.