A pH-regulated promoter for the expression of recombinant proteins inEscherichia coli
| العنوان: | A pH-regulated promoter for the expression of recombinant proteins inEscherichia coli |
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| المؤلفون: | Gerard J. Tolentino, George N. Bennett, Shi-Yuan Meng, Ka-Yiu San |
| المصدر: | Biotechnology Letters. 14:157-162 |
| بيانات النشر: | Springer Science and Business Media LLC, 1992. |
| سنة النشر: | 1992 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Growth medium, Bioengineering, General Medicine, Biology, medicine.disease_cause, Applied Microbiology and Biotechnology, Molecular biology, law.invention, Cell biology, chemistry.chemical_compound, Enzyme, Plasmid, chemistry, Regulatory sequence, law, Gene expression, Recombinant DNA, medicine, Escherichia coli, Gene, Biotechnology |
| الوصف: | A series of plasmid vectors have been developed which allows control of recombinant gene expression by altering the pH of the growth medium. Expression is controlled by the regulatory region of thecadA gene ofE. coli. Experiments using β-galactosidase as the expressed gene have resulted in an induced expression of up to 60-fold when the pH of the growth medium is lowered from pH 7 to 5.5. Expression can also be induced by switching from aerobic to anaerobic growth environment. The pH and anaerobic effects are additive boosting the expression level of β-galactosidase to a dramatic value of 200 fold. Finally, the pH-induction effect is fully reversible, a unique property which allows continuous control of gene expression using exiting pH monitoring and control equipment. |
| تدمد: | 1573-6776 0141-5492 |
| DOI: | 10.1007/bf01023351 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::75ad2bb0a0b0c67adf99a9cc10fa8c8e https://doi.org/10.1007/bf01023351 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........75ad2bb0a0b0c67adf99a9cc10fa8c8e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15736776 01415492 |
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| DOI: | 10.1007/bf01023351 |