A Broadly Conserved Deoxycytidine Deaminase Protects Bacteria from Phage Infection
| العنوان: | A Broadly Conserved Deoxycytidine Deaminase Protects Bacteria from Phage Infection |
|---|---|
| المؤلفون: | Ben J. Ridenhour, Brian Hsueh, Christopher M. Waters, Rhoades Cr, Wessel Aj, John A. Dover, Elg Ca, Kristin N. Parent, Eva M. Top, Janani Ravi, Geoffrey B. Severin |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Genetics, biology, Vibrio cholerae, Deoxycytidylate Deaminase, medicine, Bacterial genome size, biology.organism_classification, medicine.disease_cause, Gene, El Tor, Vibrio, Bacteria, Deoxycytidine deaminase |
| الوصف: | SUMMARYThe El Tor biotype of Vibrio cholerae is responsible for perpetuating the longest cholera pandemic in recorded history (1961-current). The genomic islands VSP-1 and -2 are two understudied genetic features that distinguish El Tor from previous pandemics. To understand their utility, we calculated the co-occurrence of VSP genes across bacterial genomes. This analysis predicted the previously uncharacterized vc0175, herein renamed deoxycytidylate deaminase Vibrio (dcdV), is in a gene network with dncV, a cyclic GMP-AMP synthase involved in phage defense. DcdV consists of two domains, a P-loop kinase and a deoxycytidylate deaminase, that are required for the deamination of dCTP and dCMP, inhibiting phage predation by corrupting cellular nucleotide concentrations. Additionally, DcdV is post-translationally inhibited by a unique noncoding RNA encoded 5’ of the dcdV locus. DcdV homologs are conserved in bacteria and eukaryotes and our results identify V. cholerae DcdV as the founding member of a previously undescribed bacterial phage defense system. |
| DOI: | 10.1101/2021.03.31.437871 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::3f44459b0de16a85a75adf0f4733a1c5 https://doi.org/10.1101/2021.03.31.437871 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........3f44459b0de16a85a75adf0f4733a1c5 |
| قاعدة البيانات: | OpenAIRE |
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