Periodical
Monoclonal Antibodies against Human Neurotrophin-3
| العنوان: | Monoclonal Antibodies against Human Neurotrophin-3 |
|---|---|
| المؤلفون: | Shintani, A., Watanabe, T., Kuroshima, K., Ichimori, Y., Kurokawa, T., Igarashi, K., Sasada, R. |
| المصدر: | Biochemical and Biophysical Research Communications; August 16, 1993, Vol. 194 Issue: 3 p1500-1507, 8p |
| مستخلص: | Hybridomas producing monoclonal antibodies (MoAbs) against human neurotrophin-3 (hNT-3) were established using recombinant hNT-3 produced in CHO cells and E. coli as immunogens. Of the five MoAbs obtained, MoAb 3w3 showed the highest antibody titer and also best neutralized NT-3 activity as measured by the survival of chick embryonic day-8 dorsal root ganglia neurons. A sandwich enzyme immunoassay (EIA) for NT-3 was established with solid phase MoAb 3W3 and the Fab' fragment of MoAb 3W3 conjugated to horseradish peroxidase. The detection limit was 2.7pg/well of NT-3 and no cross-reactivity with nerve growth factor up to 100ng/well was observed. Using this EIA system we have screened a variety of cell lines for NT-3 production. Among these tested, only human Burkitt's lymphoma Namalwa cells were found to be producing NT-3.Copyright 1993, 1999 Academic Press |
| قاعدة البيانات: | Supplemental Index |
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