Periodical
Construction of peptide/plasmid DNA complexes for plant gene transfection via the basic leucine zipper domain
| العنوان: | Construction of peptide/plasmid DNA complexes for plant gene transfection via the basic leucine zipper domain |
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| المؤلفون: | Nomura, Kota, Fujita, Seiya, Shimatani, Yuki, Kurita, Taichi, Thagun, Chonprakun, Abe, Naoya, Oikawa, Kazusato, Tsuchiya, Kousuke, Uji, Hirotaka, Numata, Keiji |
| المصدر: | Polymer Journal; 20240101, Issue: Preprints p1-9, 9p |
| مستخلص: | An important method for plant genetic modification is using peptide/pDNA complexes to transfer genes into plant cells. With conventional carrier peptides, the peptide sequence must contain a high amount of cationic amino acids to condense and introduce pDNA. As a result, the dissociation of pDNA from the complex is inefficient, often causing problems. Herein, we designed a new peptide carrier that mimics the basic leucine zipper (bZIP) domain of DNA-binding proteins, in which (LU)4is the leucine zipper motif and (KUA)3is the basic DNA-binding and cell-penetrating motif (U = α-aminoisobutyric acid). After (KUA)3-(LU)4peptide was mixed with pDNA, DNA molecules were condensed to form nanoparticles of approximately 130 nm. Furthermore, when complexes of (KUA)3-(LU)4peptide and pDNA were introduced into the leaves of Arabidopsis thaliana(A. thaliana), expression of the reporter protein was detected in the plant cells. Thus, (KUA)3-(LU)4peptide that mimics the bZIP domain is a novel and efficient carrier for pDNA with high dissociation efficiency. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00323896 13490540 |
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| DOI: | 10.1038/s41428-024-00901-0 |