التفاصيل البيبلوغرافية
| العنوان: |
New Iflavirus Species Characterized from Mosquitoes Captured in the Sao Paulo Zoological Facilities. |
| المؤلفون: |
Guimarães, Lilian de Oliveira, Bahia, Santana Lobato, Ribeiro, Geovani de Oliveira, Ramos, Endrya do Socorro Foro, Villanova, Fabiola, dos Santos Morais, Vanessa, Telles-de-Deus, Juliana, Helfstein, Vanessa Christe, Santos, Jesus Maia dos, Pandey, Ramendra Pati, Deng, Xutao, Delwart, Eric, Camargo-Neves, Vera Lucia Fonseca de, da Costa, Antonio Charlys, Kirchgatter, Karin, Leal, Élcio |
| المصدر: |
Microorganisms; Sep2024, Vol. 12 Issue 9, p1749, 14p |
| مصطلحات موضوعية: |
ANOPHELES, VIRAL genomes, CULEX, AEDES, SPECIES, METAGENOMICS |
| مستخلص: |
Metagenomic studies of mosquito viromes demonstrated a more diverse composition than just an exclusive composition of pathogenic arboviruses transmitted to humans. In our study, the virome of 866 female mosquitoes collected throughout 2020 at the São Paulo Zoo, located in the city of São Paulo/SP—Brazil, was obtained. Specifically, in this paper, we describe a new virus found by viral RNA extraction and next-generation MiSeq sequencing of a group of 23 specimens of Anopheles (Nys.) strodei. The complete genome with a length of 9709 nucleotides was characterized by a positive orientation and a single strand, with a single large ORF, which encodes a polyprotein of 2987 amino acids. The phylogenetic analysis showed an association with the viral family Iflaviridae and the Riboviria realm. We carried out comparisons with translated sequences of the capsid regions of other iflavirus, and the identities in relation to our sequence were below the minimum limit of 90%, indicating that possibly it is a new species of iflavirus. Our findings contribute to expanding knowledge of virome composition among mosquito species in Brazil and globally. Moreover, we provide a viral genome reference specific to this geographic region and Culicidae family of mosquitoes. This resource facilitates future in silico recognition and assembly of viral genomes within metagenomic datasets. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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