Academic Journal
Under hypoxic conditions, MSCs affect the expression and methylation level of survival‐related genes in ALL independent of apoptosis pathways in vitro.
| العنوان: | Under hypoxic conditions, MSCs affect the expression and methylation level of survival‐related genes in ALL independent of apoptosis pathways in vitro. |
|---|---|
| المؤلفون: | Marofi, Faroogh, Shomali, Navid, Younus, Laith A., Hassanzadeh, Ali, Vahedi, Ghasem, Kuznetsova, Mariya Yurievna, Solali, Saeed, Gharibi, Tohid, Hosseini, Arezoo, Mohammed, Rebar N., Mohammadi, Hamed, Tamjidifar, Rozita, Firouzi‐Amandi, Akram, Farshdousti Hagh, Majid |
| المصدر: | Biotechnology & Applied Biochemistry; Apr2022, Vol. 69 Issue 2, p822-839, 18p |
| مصطلحات موضوعية: | BONE marrow cells, MESENCHYMAL stem cells, LYMPHOBLASTIC leukemia, METHYLATION, GENE expression |
| مستخلص: | Mesenchymal stem cells (MSCs) are one of the most prominent cells in the bone marrow. MSCs can affect acute lymphocytic leukemia (ALL) cells under hypoxic conditions. With this aim, we used MOLT‐4 cells as simulators of ALL cells cocultured with bone marrow mesenchymal stem cells (BMMSCs) under hypoxic conditions in vitro. Then, mRNA and protein expression of the MAT2A, PDK1, and HK2 genes were evaluated by real‐time PCR and Western blot which was also followed by apoptosis measurement by a flow‐cytometric method. Next, the methylation status of the target genes was investigated by MS‐qPCR. Additionally, candidate gene expressions were examined after treatment with rapamycin using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. We found that the mRNA expression of the candidate genes was augmented under the hypoxic condition in which MAT2A was upregulated in cocultured cells compared to MOLT‐4, while HK2 and PDK1 were downregulated. Moreover, we found an association between gene expression and promoter methylation levels of target genes. Besides, expressions of the candidate genes were decreased, while their methylation levels were promoted following treatment with rapamycin. Our results suggest an important role for the BMMSC in regulating the methylation of genes involved in cell survival in hypoxia conditions; however, we found no evidence to prove the MSCs' effect on directing malignant lymphoblastic cells to apoptosis. [ABSTRACT FROM AUTHOR] |
| Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| قاعدة البيانات: | Complementary Index |
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