التفاصيل البيبلوغرافية
| العنوان: |
Induction of the Yersinia pestis PhoP-PhoQ Regulatory System in the Flea and Its Role in Producing a Transmissible Infection. |
| المؤلفون: |
Rebeil, Roberto1,2, Jarrett, Clayton O.1, Driver, James D.1,3, Ernst, Robert K.4,5, Oyston, Petra C. F.6, Hinnebusch, B. Joseph1 jhinnebusch@niaid.nih.gov |
| المصدر: |
Journal of Bacteriology. May2013, Vol. 195 Issue 9, p1920-1930. 11p. |
| مصطلحات موضوعية: |
*YERSINIA pestis, *FLEAS as carriers of disease, *INFECTIOUS disease transmission, *BIOFILMS, *IMMUNE response |
| مستخلص: |
Transmission of Yersinia pestis is gready enhanced after it forms a bacterial biofilm in the foregut of the flea vector that interferes with normal blood feeding. Here we report that the ability to produce a normal foregut-blocking infection depends on induction of the Y. pestis PhoP-PhoQ two-component regulatory system in the flea. Y. pestis phoP-negative mutants achieved normal infection rates and bacterial loads in the flea midgut but produced a less cohesive biofllm both in vitro and in the flea and had a greatly reduced ability to localize to and block the flea foregut. Thus, not only is the PhoP-PhoQ system induced in the flea gut environment, but also this induction is required to produce a normal transmissible infection. The altered biofilm phenotype in the flea was not due to lack of PhoPQ-dependent or PmrAB-dependent addition of aminoarabinose to the Y. pestis lipid A, because an aminoarabinose-deficient mutant that is highly sensitive to cationic antimicrobial peptides had a normal phenotype in the flea digestive tract. In addition to enhancing transmissibility, induction of the PhoP-PhoQ system in the arthropod vector prior to transmission may preadapt Y. pestis to resist the initial encounter with the mammalian innate immune response. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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