Academic Journal
子宫内膜异位症中子宫内膜间质细胞侵袭、转移能力的研究.
| العنوان: | 子宫内膜异位症中子宫内膜间质细胞侵袭、转移能力的研究. |
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| Alternate Title: | Invasion and Metastasis Ability of Endometrial Stromal Cells in Endometriosis. |
| المؤلفون: | 苏晓华1, 宋殿荣2 songdr58@126.com, 张崴2, 郭洁2, 王雅楠2, 赵琳2 |
| المصدر: | Tianjin Medical Journal. 2014, Vol. 42 Issue 12, following p1163-1167. 6p. |
| Abstract (English): | Objective To analyze the invasion and metastasis of endometrial stromal cells in endometriosis through assessing the transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA. Methods Ectopic and eutopic endometrial tissues from patients with EMs (endometriosis) or uterine fibroids were collected and cultured. The fourth generation of purified endometrial stromal cells were used for research, and the transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA were analyzed by RT-PCR. Results There were no statistical differences in transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA in eutopic endometrial stromal cells from patients with EMs or uterine fibroids between secration stage and proliferation stage（P＞0.05）. The transcription levels of COX-2, PGE2 and Snail mRNA were significantly in⁃ creased while transcription level of E-cadherin mRNA was decreased in eutopic and ectopic endometrial stromal cells in patients with EM compared with those in patients with uterine fibroid. And the difference was statistically significant（P＜0.05 or P＜0.01）. The transcription levels of COX-2, PGE2 and Snail mRNA were obviously higher while transcription level of E-cadherin mRNA was lower in ectopic endometrial stromal cells than those in eutopic endometrial stromal cells from patients with EMs. And the difference was statistically significant（P＜0.05 or P＜0.01）. Conclusion The invasion and metastasis of endometrial stromal cells of patients with EMs were remarkable than those cell of patients without EMs. What's more, the invasion and metastasis in ectopic endometrial stromal cells were more obvious than those cells in the pathogenesis of EMs. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): | 目的检测子宫内膜异位症（EMs）患者子宫内膜间质细胞中环氧化酶-2（COX-2）、前列腺素E2 （PGE2）、锌指转录因子（Snail）和E-钙黏蛋白（E-cadherin）mRNA 表达情况，分析EMs 中子宫内膜间质细胞的侵袭、 转移能力。方法收集EMs 患者的在位及异位病灶子宫内膜组织和子宫肌瘤患者的在位内膜组织，原代消化培养 子宫内膜细胞，取纯化的第4 代子宫内膜间质细胞进行研究，RT-PCR 法检测细胞COX-2、PGE2、Snail 和E-cadherin mRNA 的表达。结果EMs 和子宫肌瘤患者分泌期在位子宫内膜间质细胞COX-2、PGE2、Snail 和E-cadherin mRNA 表达与增殖期比较差异均无统计学意义；EMs 患者在位及异位的子宫内膜间质细胞COX-2、PGE2、Snail mRNA 表达比肌瘤患者在位细胞均明显升高，而E-cadherin mRNA 表达降低（P＜0.05 或P＜0.01）；EMs 患者异位的 子宫内膜间质细胞COX-2、PGE2、Snail mRNA 表达比在位间质细胞升高，而E-cadherin mRNA 表达降低（P＜0.05 或 P＜0.01）。结论EMs 患者的子宫内膜间质细胞侵袭、转移能力强于非EMs 患者，且在EMs 发病中异位病灶子宫内 膜间质细胞的侵袭、转移能力更强。 [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: | Academic Search Index |
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Array ( [Name] => TitleAlt [Label] => Alternate Title [Group] => TiAlt [Data] => Invasion and Metastasis Ability of Endometrial Stromal Cells in Endometriosis. ) Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22苏晓华%22">苏晓华</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22宋殿荣%22">宋殿荣</searchLink><relatesTo>2</relatesTo><i> songdr58@126.com</i><br /><searchLink fieldCode="AR" term="%22张崴%22">张崴</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22郭洁%22">郭洁</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22王雅楠%22">王雅楠</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22赵琳%22">赵琳</searchLink><relatesTo>2</relatesTo> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => <searchLink fieldCode="JN" term="%22Tianjin+Medical+Journal%22">Tianjin Medical Journal</searchLink>. 2014, Vol. 42 Issue 12, following p1163-1167. 6p. ) Array ( [Name] => Abstract [Label] => Abstract (English) [Group] => Ab [Data] => Objective To analyze the invasion and metastasis of endometrial stromal cells in endometriosis through assessing the transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA. Methods Ectopic and eutopic endometrial tissues from patients with EMs (endometriosis) or uterine fibroids were collected and cultured. The fourth generation of purified endometrial stromal cells were used for research, and the transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA were analyzed by RT-PCR. Results There were no statistical differences in transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA in eutopic endometrial stromal cells from patients with EMs or uterine fibroids between secration stage and proliferation stage（P＞0.05）. The transcription levels of COX-2, PGE2 and Snail mRNA were significantly in⁃ creased while transcription level of E-cadherin mRNA was decreased in eutopic and ectopic endometrial stromal cells in patients with EM compared with those in patients with uterine fibroid. And the difference was statistically significant（P＜0.05 or P＜0.01）. The transcription levels of COX-2, PGE2 and Snail mRNA were obviously higher while transcription level of E-cadherin mRNA was lower in ectopic endometrial stromal cells than those in eutopic endometrial stromal cells from patients with EMs. And the difference was statistically significant（P＜0.05 or P＜0.01）. Conclusion The invasion and metastasis of endometrial stromal cells of patients with EMs were remarkable than those cell of patients without EMs. What's more, the invasion and metastasis in ectopic endometrial stromal cells were more obvious than those cells in the pathogenesis of EMs. [ABSTRACT FROM AUTHOR] ) Array ( [Name] => Abstract [Label] => Abstract (Chinese) [Group] => Ab [Data] => 目的检测子宫内膜异位症（EMs）患者子宫内膜间质细胞中环氧化酶-2（COX-2）、前列腺素E2 （PGE2）、锌指转录因子（Snail）和E-钙黏蛋白（E-cadherin）mRNA 表达情况，分析EMs 中子宫内膜间质细胞的侵袭、 转移能力。方法收集EMs 患者的在位及异位病灶子宫内膜组织和子宫肌瘤患者的在位内膜组织，原代消化培养 子宫内膜细胞，取纯化的第4 代子宫内膜间质细胞进行研究，RT-PCR 法检测细胞COX-2、PGE2、Snail 和E-cadherin mRNA 的表达。结果EMs 和子宫肌瘤患者分泌期在位子宫内膜间质细胞COX-2、PGE2、Snail 和E-cadherin mRNA 表达与增殖期比较差异均无统计学意义；EMs 患者在位及异位的子宫内膜间质细胞COX-2、PGE2、Snail mRNA 表达比肌瘤患者在位细胞均明显升高，而E-cadherin mRNA 表达降低（P＜0.05 或P＜0.01）；EMs 患者异位的 子宫内膜间质细胞COX-2、PGE2、Snail mRNA 表达比在位间质细胞升高，而E-cadherin mRNA 表达降低（P＜0.05 或 P＜0.01）。结论EMs 患者的子宫内膜间质细胞侵袭、转移能力强于非EMs 患者，且在EMs 发病中异位病灶子宫内 膜间质细胞的侵袭、转移能力更强。 [ABSTRACT FROM AUTHOR] ) |
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