نتائج البحث - "oxygen concentration measurements"

المساهمون: MARY ANN LIEBERT, INC, Zuncheddu, Daniele, Schwab, Andrea, Della Bella, Elena, Generelli, Silvia, Kurth, Felix, Serra, Pier Andrea, Rocchitta, Gaia Giovanna Maria, Kasper, Hermann, Grad, Sibylle, Basoli, Valentina

مصطلحات موضوعية: The importance of oxygen concentrations in cell culture for tissue engineering and regenerative medicine is often underestimated1, although this parameter is crucial for cell fate during differentiation. The environmental oxygen concentration (20.9%) does not represent the physiological condition within human tissue2, such as bone and cartilage. For this reason, real time oxygen monitoring can improve the standardization of stem cell differentiation outcome, both for two‐dimensional (2D) and three‐dimensional (3D) methods. Therefore, we developed electrochemical oxygen sensors, already successfully applied in other fields3,4, using a copper wire inserted into a silica capillary tube, partly filled with a graphite‐loaded epoxy resin. The sensors were introduced into the tissue plates through modified tissue plate lids, and the detection of oxygen was obtained by means of an electrochemical reduction, i.e. applying a cathodic potential to the carbon‐disk surface. We tested the sensor stability in cell culture medium and its material cytotoxicity for bone marrow stem cells (BMSCs) cultures. The sensors are proved to be non‐cytotoxic for up to 7 days. and were not damaged over a period of 21 days in cell culture medium The sensors were subsequently used to analyze oxygen concentration in 3D cultures of BMSCs embedded in two different hydrogels, gelatin methacryloyl (GelMa) and tyramine functionalized hyaluronic acid (HAT). The embedded stem cells were differentiated in chondrogenic medium for 21 days, both under hypoxic and normal conditions. The proposed system has the potential to represent an easy way of in real time oxygen concentration measurements in 3D constructs during differentiation

Relation: info:eu-repo/semantics/altIdentifier/wos/WOS:000821187301164; ispartofbook:TISSUE ENGINEERING PART A; 6th World Congress of the Tissue-Engineering-and-Regenerative-Medicine-International-Society (TERMIS); volume:28; firstpage:S268; lastpage:S269; journal:TISSUE ENGINEERING, PART A; alleditors:MARY ANN LIEBERT, INC; https://hdl.handle.net/11388/303927

الاتاحة: https://hdl.handle.net/11388/303927
https://doi.org/10.1089/ten.tea.2022.29025.abstracts

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Video_3_Hypoxia Onset in Mesenchymal Stem Cell Spheroids: Monitoring With Hypoxia Reporter Cells.MP4

المؤلفون: Carola Schmitz, Ekaterina Potekhina, Teresa Irianto, Vsevolod V. Belousov, Antonina Lavrentieva

مصطلحات موضوعية: Biotechnology, Biological Engineering, Genetic Engineering, Biomarkers, Biomaterials, Biomechanical Engineering, Biomedical Engineering not elsewhere classified, Synthetic Biology, Agricultural Marine Biotechnology, Bioremediation, Bioprocessing, Bioproduction and Bioproducts, Industrial Biotechnology Diagnostics (incl. Biosensors), Industrial Microbiology (incl. Biofeedstocks), Industrial Molecular Engineering of Nucleic Acids and Proteins, Industrial Biotechnology not elsewhere classified, Medical Biotechnology Diagnostics (incl. Biosensors), Medical Molecular Engineering of Nucleic Acids and Proteins, Regenerative Medicine (incl. Stem Cells and Tissue Engineering), Medical Biotechnology not elsewhere classified, hypoxia sensor, cell spheroids, adipose tissue-derived mesenchymal stem cells, hypoxia reporter cells, oxygen concentration measurements

Relation: https://figshare.com/articles/media/Video_3_Hypoxia_Onset_in_Mesenchymal_Stem_Cell_Spheroids_Monitoring_With_Hypoxia_Reporter_Cells_MP4/20223957

الاتاحة: https://doi.org/10.3389/fbioe.2021.611837.s004
https://figshare.com/articles/media/Video_3_Hypoxia_Onset_in_Mesenchymal_Stem_Cell_Spheroids_Monitoring_With_Hypoxia_Reporter_Cells_MP4/20223957

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Image

Image_1_Hypoxia Onset in Mesenchymal Stem Cell Spheroids: Monitoring With Hypoxia Reporter Cells.JPEG

المؤلفون: Carola Schmitz, Ekaterina Potekhina, Teresa Irianto, Vsevolod V. Belousov, Antonina Lavrentieva

مصطلحات موضوعية: Biotechnology, Biological Engineering, Genetic Engineering, Biomarkers, Biomaterials, Biomechanical Engineering, Biomedical Engineering not elsewhere classified, Synthetic Biology, Agricultural Marine Biotechnology, Bioremediation, Bioprocessing, Bioproduction and Bioproducts, Industrial Biotechnology Diagnostics (incl. Biosensors), Industrial Microbiology (incl. Biofeedstocks), Industrial Molecular Engineering of Nucleic Acids and Proteins, Industrial Biotechnology not elsewhere classified, Medical Biotechnology Diagnostics (incl. Biosensors), Medical Molecular Engineering of Nucleic Acids and Proteins, Regenerative Medicine (incl. Stem Cells and Tissue Engineering), Medical Biotechnology not elsewhere classified, hypoxia sensor, cell spheroids, adipose tissue-derived mesenchymal stem cells, hypoxia reporter cells, oxygen concentration measurements

Relation: https://figshare.com/articles/figure/Image_1_Hypoxia_Onset_in_Mesenchymal_Stem_Cell_Spheroids_Monitoring_With_Hypoxia_Reporter_Cells_JPEG/20223948

الاتاحة: https://doi.org/10.3389/fbioe.2021.611837.s001
https://figshare.com/articles/figure/Image_1_Hypoxia_Onset_in_Mesenchymal_Stem_Cell_Spheroids_Monitoring_With_Hypoxia_Reporter_Cells_JPEG/20223948

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Hypoxia Onset in Mesenchymal Stem Cell Spheroids: Monitoring With Hypoxia Reporter Cells

المؤلفون: Carola Schmitz, Ekaterina Potekhina, Vsevolod V. Belousov, Antonina Lavrentieva

المصدر: Frontiers in Bioengineering and Biotechnology
Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
Frontiers in Bioengineering and Biotechnology 9 (2021)

مصطلحات موضوعية: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie, ddc:570, lcsh:Biotechnology, lcsh:TP248.13-248.65, hypoxia sensor, hypoxia reporter cells, Bioengineering and Biotechnology, adipose tissue-derived mesenchymal stem cells, cell spheroids, oxygen concentration measurements, Original Research