المؤلفون: Humberto De Vitto, Kafi K. J. Belfon, Nandini Sharma, Sarah Toay, Jan Abendroth, David. M. Dranow, Christine M. Lukacs, Ryan Choi, Hannah S. Udell, Sydney Willis, George Barrera, Olive Beyer, Teng Da Li, Katherine A. Hicks, Andrew T. Torelli, Jarrod B. French

مصطلحات موضوعية: Biophysics, Biochemistry, Cell Biology, Genetics, Molecular Biology, Developmental Biology, Cancer, Infectious Diseases, Plant Biology, Computational Biology, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, ubiquitous molecules involved, resolution crystal structures, many cellular processes, kinetics analysis indicates, display phosphatase activity, de novo <, biosynthesize thiamin employ, 50 , pyridoxal kinase enzymes, 5 -( 2, pyridoxal phosphate thiamin, generate thiamin phosphate, thiamin employs, pyridoxal 5, phosphate derivatives, weak inhibitor, parallel synthesis, noncovalently bound

Relation: https://figshare.com/articles/journal_contribution/Characterization_of_an_Acinetobacter_baumannii_Monofunctional_Phosphomethylpyrimidine_Kinase_That_Is_Inhibited_by_Pyridoxal_Phosphate/25135055

الاتاحة: https://doi.org/10.1021/acs.biochem.3c00640.s001
https://figshare.com/articles/journal_contribution/Characterization_of_an_Acinetobacter_baumannii_Monofunctional_Phosphomethylpyrimidine_Kinase_That_Is_Inhibited_by_Pyridoxal_Phosphate/25135055

المؤلفون: Humberto De Vitto, Kafi K. J. Belfon, Nandini Sharma, Sarah Toay, Jan Abendroth, David. M. Dranow, Christine M. Lukacs, Ryan Choi, Hannah S. Udell, Sydney Willis, George Barrera, Olive Beyer, Teng Da Li, Katherine A. Hicks, Andrew T. Torelli, Jarrod B. French

مصطلحات موضوعية: Biophysics, Biochemistry, Cell Biology, Genetics, Molecular Biology, Developmental Biology, Cancer, Infectious Diseases, Plant Biology, Computational Biology, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, ubiquitous molecules involved, resolution crystal structures, many cellular processes, kinetics analysis indicates, display phosphatase activity, de novo <, biosynthesize thiamin employ, 50 , pyridoxal kinase enzymes, 5 -( 2, pyridoxal phosphate thiamin, generate thiamin phosphate, thiamin employs, pyridoxal 5, phosphate derivatives, weak inhibitor, parallel synthesis, noncovalently bound

Relation: https://figshare.com/articles/journal_contribution/Characterization_of_an_Acinetobacter_baumannii_Monofunctional_Phosphomethylpyrimidine_Kinase_That_Is_Inhibited_by_Pyridoxal_Phosphate/25135055

الاتاحة: https://doi.org/10.1021/acs.biochem.3c00640.s001

المؤلفون: Lin Shi, Haiying Cai, Han Wang, Qiwei Wang, Lili Shi, Tao Li

مصطلحات موضوعية: Biophysics, Cell Biology, Molecular Biology, Biotechnology, Cancer, Science Policy, Biological Sciences not elsewhere classified, target mirna induces, requiring rna extraction, many cellular processes, cell apoptosis analysis, attracted increasing interest, cancer cell growth, release internal mirnas, enhanced ecl readout, close proximity state, another fna structure, 21 negatively participates, 2 , cancer cells, shaped junction, fna structures, facile way, effective capturing, effective capture, covalently hemin, conventional counterparts, cd63 protein, apoptotic process, 21 level

Relation: https://figshare.com/articles/journal_contribution/Proximity-Enhanced_Electrochemiluminescence_Sensing_Platform_for_Effective_Capturing_of_Exosomes_and_Probing_Internal_MicroRNAs_Involved_in_Cancer_Cell_Apoptosis/24625691

الاتاحة: https://doi.org/10.1021/acs.analchem.3c03412.s001
https://figshare.com/articles/journal_contribution/Proximity-Enhanced_Electrochemiluminescence_Sensing_Platform_for_Effective_Capturing_of_Exosomes_and_Probing_Internal_MicroRNAs_Involved_in_Cancer_Cell_Apoptosis/24625691

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/journal_contribution/Sequences_of_PCR_products_amplified_from_the_region_surrounding_the_3_end_of_the_inserted_donor_DNA_in_LF5-HA_strains_related_to_Figs_6_and_7_of_main_text_/21705073

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.s002

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/journal_contribution/Sequences_of_PCR_products_for_1_initial_screening_of_LF5-HA_strains_related_to_Fig_5_of_main_text_and_2_to_check_integrity_of_3_UTR_of_LF5-HA_strains_related_to_Fig_7_of_main_text_/21705070

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.s001

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/journal_contribution/Schematic_representation_of_vector_construction_/21705088

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.s007

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/journal_contribution/Sequences_of_the_PCR_products_for_initial_screening_of_NAP1L1-tagged_strains_related_to_Fig_8_of_main_text_/21705076

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.s003

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/journal_contribution/Plasmid_sequences_/21705082

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.s005

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/journal_contribution/List_of_gRNAs_and_primers_/21705085

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.s006

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/journal_contribution/Sequences_of_the_PCR_products_amplified_from_the_region_surrounding_the_5_end_of_the_inserted_donor_DNA_in_NAP1L1-tagged_strains_related_to_Fig_9_of_main_text_/21705079

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.s004

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/journal_contribution/PCR_analysis_of_transformants_that_yielded_no_band_during_initial_PCR_screen_for_LF5-tagged_strains_confirmed_that_the_i_LF5_i_locus_was_disrupted_/21705091

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.s008

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/journal_contribution/Both_NAP1L1_RNP1_and_RNP2_cut_i_NAP1L1_i_DNA_targets_i_in_vitro_i_/21705094

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.s009

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/journal_contribution/Original_western_blot_and_agarose_gel_images_/21705895

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.s010

المؤلفون: William H. Skinner (11524018), Michael Chung (4725162), Stephen Mitchell (596102), Asli Akidil (11524021), Kristin Fabre (11524024), Richard Goodwin (7374011), Adam A. Stokes (5229716), Norbert Radacsi (1904611), Colin J. Campbell (1606291)

مصطلحات موضوعية: Biochemistry, Microbiology, Cell Biology, Molecular Biology, Physiology, Biotechnology, Cancer, Inorganic Chemistry, Hematology, Virology, Space Science, Environmental Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, responsive reporter 4, many cellular processes, important chemical factor, enhanced raman scattering, controlled cell death, coated polymer mesh, reproducible sers spectra, study extracellular ph, phe via surface, extracellular microenvironment, concept study, vitro <, unique platform, time measurement, subtle changes

Relation: https://figshare.com/articles/journal_contribution/A_SERS-Active_Electrospun_Polymer_Mesh_for_Spatially_Localized_pH_Measurements_of_the_Cellular_Microenvironment/16744093

الاتاحة: https://doi.org/10.1021/acs.analchem.1c02530.s001

المؤلفون: Anish R. Roy (11496192), Wei Zhang (405), Zeinab Jahed (805854), Ching-Ting Tsai (11230439), Bianxiao Cui (542576), W. E. Moerner (185394)

مصطلحات موضوعية: Biophysics, Biochemistry, Cell Biology, Physiology, Biotechnology, Infectious Diseases, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, relative spatial position, optical diffraction limit, minimize spherical aberrations, many cellular processes, limited axial detail, electron microscopy measurements, defined membrane curvatures, actin preferentially accumulate, oil immersion objective, cytoplasmic protein ap, dimensional fluorescence microscopy, 3d sr method, localization microscopy, membrane curvature, general method, actin rearrangements, used three, strongly influence, specific threshold, r <, pillar caps, molecule super

Relation: https://figshare.com/articles/journal_contribution/Exploring_Cell_Surface_Nanopillar_Interactions_with_3D_Super-Resolution_Microscopy/16695504

الاتاحة: https://doi.org/10.1021/acsnano.1c05313.s001

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/figure/LF5-HA_protein_is_expressed_and_localized_normally_in_8_out_of_10_PCR-positive_clones_/21705913

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.g006

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/figure/Schematic_overview_of_TIM-based_N-terminal_tagging_strategy_using_two_gRNAs_/21705907

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.g004

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/figure/Initial_PCR_screening_of_transformants_from_the_LF5-HA_C-terminal_tagging_experiment_/21705910

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.g005

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/figure/PCR_screening_of_transformants_for_insertion_of_the_5_end_of_the_NAP1L1_mNeonGreen-3xFLAG_donor_DNA_at_the_RNP1_cut_site_/21705922

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.g009

المؤلفون: Yuqing Hou (725544), Xi Cheng (296756), George B. Witman (1327854)

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, targeted insertional mutagenesis, mutant cell line, many cellular processes, involve transforming either, important model organism, exogenous dna construct, western blotting confirmed, normal length flagella, normal cell size, increasingly indispensable tool, immunofluorescence microscopy showed, tagged cell line, including essential genes, tagging strategy promises, strategy utilizes tim, chlamydomonas reinhardtii <, appear wild type, type tagged protein, protein tagging

Relation: https://figshare.com/articles/figure/PCR_screening_of_transformants_for_insertion_of_the_3_end_of_the_NAP1L1_mNeonGreen-3xFLAG_donor_DNA_at_the_RNP2_cut_site_/21705919

الاتاحة: https://doi.org/10.1371/journal.pone.0278972.g008