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1Conference
المؤلفون: Palma Simoniello, Alexander Helm, Walter Tinganelli, Fuki Kurosawa, Claudia Fournier, Takashi Shimokawa and Marco Durante.
المساهمون: Palma Simoniello, Alexander Helm , Walter Tinganelli , Fuki Kurosawa , Claudia Fournier , Takashi Shimokawa and Marco Durante ., Simoniello, Palma, Helm, Alexander, Tinganelli, Walter, Kurosawa, Fuki, Fournier, Claudia, Durante., Takashi Shimokawa and Marco
مصطلحات موضوعية: Radiotherapy is one of the main treatments for cancers, which has experienced important improvements in the last year, metastatic disease still remains a problem. It is known that immune system plays a key role in the elimination of primary tumors and preventing tumor recurrence. Ionizing radiation is reported to be immunogenic, i.e. it may induce an immunogenic cell death in tumor cells leading to immunoactivation, in turn resulting in the shrinkage of out-of-field metastases. This phenomenon is known as abscopal effect. The right combination of radiotherapy with immunotherapy could be a strategy to induce durable responses and improve survival. Particle therapy with protons and especially carbon-ions is assumed to further increase the immunogenic effect. The aim of this study was to investigate the efficacy of X-ray and high-energy carbon ion therapy in a combination with immunotherapy. In this work we investigated the combination of radiotherapy, with either carbon ions or X-rays, and immune checkpoint inhibitors (CPI) (anti- CTLA-4 and anti-PD-1), in a syngeneic osteosarcoma mouse model (C3H/He mice, LM8 cells). Tumor cells were injected in both hind limbs of the mice. Following an initial growth period, one of the tumors was irradiated while the other was kept out of the irradiation field, mimicking the abscopal tumor. Macroscopic analysis on out-of-field metastases were carried out to investigate spontaneously formed lung metastases. Immune responses were investigated by histological analyses on the abscopal tumors screening. The results show that combination of carbon ion therapy and CPI lead to a reduced growth of abscopal tumors and reduced formation of lung metastases. In addition, in the abscopal tumors treated with combining therapy an immunogenic response resulting in increasing of infiltration of CD8+ cells was observed. Combination of CPI with high-energy carbon ion radiotherapy could be a promising strategy for the treatment of tumor and metastasis.
Relation: ispartofbook:XIX Congresso nazionale SIRR Società Italiana per le Ricerche sulle Radiazioni; XIX Congresso nazionale SIRR Società Italiana per le Ricerche sulle Radiazioni; lastpage:12; numberofpages:1; http://hdl.handle.net/11367/89111
الاتاحة: http://hdl.handle.net/11367/89111
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2
المؤلفون: Simoniello, Palma, Alexander, Helm, Walter, Tinganelli, Fuki, Kurosawa, Claudia, Fournier, Takashi Shimokawa and Marco, Durante.
مصطلحات موضوعية: Radiotherapy is one of the main treatments for cancers, in turn resulting in the shrinkage of out-of-field metastases. This phenomenon is known as abscopal effect. The right combination of radiotherapy with immunotherapy could be a strategy to induce durable responses and improve survival. Particle therapy with protons and especially carbon-ions is assumed to further increase the immunogenic effect. The aim of this study was to investigate the efficacy of X-ray and high-energy carbon ion therapy in a combination with immunotherapy. In this work we investigated the combination of radiotherapy, i.e. it may induce an immunogenic cell death in tumor cells leading to immunoactivation, LM8 cells). Tumor cells were injected in both hind limbs of the mice. Following an initial growth period, metastatic disease still remains a problem. It is known that immune system plays a key role in the elimination of primary tumors and preventing tumor recurrence. Ionizing radiation is reported to be immunogenic, Radiotherapy is one of the main treatments for cancers, which has experienced important improvements in the last years, metastatic disease still remains a problem. It is known that immune system plays a key role in the elimination of primary tumors and preventing tumor recurrence. Ionizing radiation is reported to be immunogenic, i.e. it may induce an immunogenic cell death in tumor cells leading to immunoactivation, in turn resulting in the shrinkage of out-of-field metastases. This phenomenon is known as abscopal effect. The right combination of radiotherapy with immunotherapy could be a strategy to induce durable responses and improve survival. Particle therapy with protons and especially carbon-ions is assumed to further increase the immunogenic effect. The aim of this study was to investigate the efficacy of X-ray and high-energy carbon ion therapy in a combination with immunotherapy. In this work we investigated the combination of radiotherapy, with either carbon ions or X-rays, and immune checkpoint inhibitors (CPI) (anti- CTLA-4 and anti-PD-1), in a syngeneic osteosarcoma mouse model (C3H/He mice, LM8 cells). Tumor cells were injected in both hind limbs of the mice. Following an initial growth period, one of the tumors was irradiated while the other was kept out of the irradiation field, mimicking the abscopal tumor. Macroscopic analysis on out-of-field metastases were carried out to investigate spontaneously formed lung metastases. Immune responses were investigated by histological analyses on the abscopal tumors screening. The results show that combination of carbon ion therapy and CPI lead to a reduced growth of abscopal tumors and reduced formation of lung metastases. In addition, in the abscopal tumors treated with combining therapy an immunogenic response resulting in increasing of infiltration of CD8+ cells was observed. Combination of CPI with high-energy carbon ion radiotherapy could be a promising strategy for the treatment of tumor and metastasis, in a syngeneic osteosarcoma mouse model (C3H/He mice, one of the tumors was irradiated while the other was kept out of the irradiation field, and immune checkpoint inhibitors (CPI) (anti- CTLA-4 and anti-PD-1), with either carbon ions or X-rays, which has experienced important improvements in the last years, mimicking the abscopal tumor. Macroscopic analysis on out-of-field metastases were carried out to investigate spontaneously formed lung metastases. Immune responses were investigated by histological analyses on the abscopal tumors screening. The results show that combination of carbon ion therapy and CPI lead to a reduced growth of abscopal tumors and reduced formation of lung metastases. In addition, in the abscopal tumors treated with combining therapy an immunogenic response resulting in increasing of infiltration of CD8+ cells was observed. Combination of CPI with high-energy carbon ion radiotherapy could be a promising strategy for the treatment of tumor and metastasis
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3
المؤلفون: Ni Liu, Dan Pi, Chun-Mei Liu
المصدر: Journal of Hainan Medical University, Vol 24, Iss 01, Pp 5-8 (2018)
مصطلحات موضوعية: GRP78, endocrine system diseases, 409000. Fund Project: Science and Technology Project of Qianjiang District No: 2016056. 1. Introduction Ovarian cancer is one of the most common malignant tumors in the female reproductive system. Surgical resection and chemotherapy are the main treatments. Because ovarian cancer cells are more sensitive to chemotherapy drugs, Proliferation, DMEM, RIPA lysate was purchased in Shanghai Beyotime Company, lcsh:Medicine, Invasion, Chongqing, Ovarian cancer, especially platinum drugs, and enzyme-linked immunosorbent assay kits were purchased from Shanghai Westang Biotechnology Company. Objective: To study the effect of metformin combined with cisplatin on the expression of genes related to ovarian cancer cell growth and invasion in vitro. Methods: SKOV3 ovarian cancer cell lines were cultured and randomly divided into the control group treated with serumfree DMEM, the platinum-based intravenous chemotherapy is widely used in the treatment of advanced ovarian cancer[1, CyclinD1, the MET group treated with serum-free DMEM containing 10 mmol/L metformin and the DDP+MET group treated with serum-free DMEM containing 10 μmol/L cisplatin and 10 mmol/L metformin. Results: 24 h after treatment, some patients with ovarian cancer can develop drug resistance during chemotherapy, lcsh:R, TBX2, it has been confirmed in recent years that it has anti-tumor effect and chemotherapy drug sensitization effect, the growth and invasion of cancer cells involve the changes in the expression of multiple proliferationrelated genes and invasion-related genes. In the following studies, Metformin, the DDP group treated with serum-free DMEM containing 10 μmol/L cisplatin, Department of Obstetrics and Gynecology, which will affect the efficacy of chemotherapy[3]. Metformin is a kind of insulin sensitizer for diabetes treatment, Ki-67, Chongqing Qianjiang National Hospital, and its combination with a variety of chemotherapy drugs can enhance the killing effect of chemotherapy drugs on tumor cells[4]. In the progression of the course of ovarian cancer, Cisplatin, we specifically analyzed the effects of metformin combined with cisplatin on the expression of genes associated with ovarian cancer cell growth and invasion in vitro. 2. Experimental materials and methods 2.1 Experimental materials Ovarian cancer SKOV3 cell lines were bought in the cell bank of Chinese Academy of Sciences, fetal bovine serum and 0.125% trypsin for cell culture were purchased in Hyclone Company, cisplatin and metformin were bought in the Sigma Company
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4
المؤلفون: Hai-Jing Wang, Qian Yan
المصدر: TMR Non-Drug Therapy, Vol 1, Iss 4, Pp 151-158 (2018)
مصطلحات موضوعية: physical training and drug therapy are mainly used to treat CP in children at home and abroad. However, Surgical treatment, Rehabilitation treatment, Research progress, causes significant mental stress, this article analyzes the main treatments used, stem cell therapy, seriously affects the quality of life, Cell therapy, drug therapy, surgery, and therapies used over the previous decades is limited. The use of more appropriate comprehensive treatment that involves improving the quality of life to social participation is helpful and meaningful for children with CP and their carers. Based on the literature and guidelines from home and abroad, including rehabilitation treatment, plentiful evidence has shown that the efficacy of drugs, lcsh:Therapeutics. Psychotherapy, lcsh:RC475-489, Cerebral palsy, and cannot be cured. It has a detrimental effect on families of children with CP, and compares the advantages and disadvantages of various treatments. It provides a reference for patients with CP to help choose treatment methods and a new idea for research on the treatment of CP. Keywords: Cerebral palsy, and the treatment of complications, and places a great economic burden on society. At present
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5Academic Journal
المؤلفون: Wei Huang (36889), Xiaoyue Wu (2926011), Yifan Zhao (659636), Yanhua Liu (164798), Bo Zhang (6559), Mingxin Qiao (14335037), Zhou Zhu (46647), Zhihe Zhao (4034060)
مصطلحات موضوعية: Biochemistry, Medicine, Cell Biology, Pharmacology, Biotechnology, Cancer, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, biological experiment part, automatically programmed delivery, significant inhibitory effect, drug release experiment, degradation experiment reflected, residual tumor cells, postoperative bone tumor, inhibit tumor recurrence, programmed differential release, programmed release, bone tumor, positive effect, gradient degradation, differential release, bone repair, bone defects, vivo <, vitro <, material characterization, main treatments, large area
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6Academic Journal
المؤلفون: Sheng Xu (400895), Huanhuan Tan (6701453), Qianqian Yang (203850), Riyuan Wang (4344958), Chi Tian (6921479), Yanting Ji (6921482), Ping Zhao (47228), Qingyou Xia (47221), Feng Wang (44414)
مصطلحات موضوعية: Biochemistry, Genetics, Pharmacology, Biotechnology, Immunology, Hematology, Space Science, Chemical Sciences not elsewhere classified, transgenic silkworm strain, sheet internal structure, multiple biofunctional protein, intestinal flora family, carrying silk cocoons, caused side effects, induced immunosuppression chemotherapy, sufficient hlf resource, side effects, caused immunosuppression, induced imbalance, two problems, thus prolonging, sustainable release, splenic follicles, solution form, small intestine, simultaneously solve, results suggest, oral administration, main treatments, immunoregulatory factors